Buradasınız

Anasayfa » Content

SIÇAN TİROİD BEZİNDE KADMİYUM HASARINA KARŞI C VİTAMİNİ, E VİTAMİNİ VE SELENYUMUN KOMBİNE KULLANIMININ ETKİLERİ

Journal Name:

  • Cerrahpaşa Tıp Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Histopathological alterations to various organs of the different species in acute and chronic cadmium exposure have been studied by light and electron microscopy. Metallothionein is a protein inducible by heavy metal ions. The aim of this study was to determine the effects of vitamin C, vitamin E and selenium combination on the cadmium-induced morphological and immunohistochemical changes in the thyroid gland of adult male rats. Results.- In the group given cadmium, the degenerative changes were observed in the thyroid gland. In addition, an increase in the number of metallothionein positive cells was detected in this group. These immunoreactive cells were identified as mast cells. While these degenerative changes and metallothionein immunoreactive cells in the thyroid gland were decreased in the group given cadmium and antioxidants according to the experimental group.
Bookmark/Search this post with
Abstract (Original Language): 
Çalışmanın amacı sıçan tiroid bezinde kad-miyumun sebep olduğu hasara karşı C vitami-ni, E vitamini ve selenyumun koruyucu bir et-kisinin olup olmadığını incelemek ve metallo-tionein ekspresyonunu tespit etmekti. Grup I: Dokunulmamış sıçanlardan; Grup II: 8 gün oral olarak sadece C vitamini (250 mg/kg/gün), E vitamini (250 mg/kg/gün) ve sodyum selenat (0.25 mg/kg/gün) verilen sıçanlardan; Grup III: İntraperitoneal olarak CdCl2 (2 mg/kg/gün) ve-rilen sıçanlardan; Grup IV: Kadmiyum veril-mesinden 1 saat önce, aynı zaman ve dozda C vitamini, E vitamini ve selenyum verilen sıçan-lardan oluşturuldu. Sıçanların tiroid bezinden alınan doku parçaları rutin takipleri yapıldıktan sonra, histolojik ve immunohistokimyasal ola-rak incelendi. Sonuç olarak, kadmiyumun tiro-id bezinde yapısal dejeneratif değişikliklere se-bep olduğunu, bu antioksidanların tiroid be-zinde kadmiyum ile oluşan oksidatif hasara karşı koruyucu bir etki gösterdiğini ve metallo-tionein ekspresyonunun düzenlenmesine katkı-da bulunabileceğini söyleyebiliriz. Ayrıca, çok sayıda metallotionein pozitif hücre içeren tiro-id bezinin kadmiyum birikimi ile ilgili olduğu ve metallotioneinin de bu birikimde rolu oldu-ğu düşünülmektedir.
FULL TEXT (PDF): 
PDF icon arastirmax-sican-tiroid-bezinde-kadmiyum-hasarina-karsi-c-vitamini-e-vitamini-selenyumun-kombine-kullaniminin-etkileri.pdf
  • 4
174-180
  • Turkish

REFERENCES

References: 

1. Ohta H, Cherian MG. Gastrointestinal absorption of cadmium and metallothionein. Toxicol Applied Pharmacol 1991; 107: 63-72.
2. Nigam D, Shukla GS, Agarwal AK. Glutathione depletion and oxidative damage in mitochondria
CERRAHPAŞA TIP DERGİSİ Cilt (Sayı) 35 (4)
180
following exposure to cadmium in rat liver and kidney. Toxicol Lett 1999; 106: 151-157.
3. Yiin SJ, Chern Cl, Sheu JY et al. Cadmium-induced renal lipid peroxidation in rats and protection by selenium. J Toxicol Environ Health 1999; 57: 403-413.
4. Watari N, Hotta Y, Mabuchi Y. Ultrastructural studies on a cadmium-storing cell in rat pancreatic tissues following cadmium chloride administration. J Electron Microsc 1989; 38: 235-241.
5. Griffin JL, Walker LA, Shore RF, Nicholson JK. Metabolic profiling of chronic cadmium exposure in the rat. Chem Res Toxicol 2001; 14: 1428-1434.
6. Demir N, Akkoyunlu G, Agar A, Yargicoglu P, Tanriover G, Demir R. Effect of cadmium on sciatic nerve in diabetic rats: An ultrastructural study. Int J Neurosci 2002; 112: 779-796.
7. Asar M, Kayisli UA, Izgut-Uysal VN, Akkoyunlu G. Immunohistochemical and ultrastructural changes in the renal cortex of cadmium-treated rats. Biol Trace Elem Res 2004; 97: 249-263.
8. Yiin SJ, Chern CL, Sheu JY, Lin TH. Cadmium induced lipid peroxidation in rat testes and protection by selenium. Biometals 1999; 12: 353-359.
9. Nigam D, Shukla GS, Agarwal AK. Glutathione depletion and oxidative damage in mitochondria following exposure to cadmium in rat liver and kidney. Toxicol Lett 1999; 106: 151-157.
10. Fasanya-Odewumi C, Latinwo LM, Ikediobi C et al. The genotoxicity and cytotoxicity of dermally-administered cadmium: Effects of dermal cadmium administration. Int J Mol Med 1998; 1: 1001-1006.
11. Shaikh ZA, Vu TT, Zaman K. Oxidative stress as a mechanism of chronic cadmium-induced hepatotoxicity and renal toxicity and protection by antioxidants. Toxicol Applied Pharmacol 1999; 154: 256-263.
12. Szczurek EI, Bjornsson CS, Taylor CG. Dietary zinc deficiency and repletion modulate metallothionein immunolocalization and concentration in small intestine and liver of rats. J Nutr 2001; 131: 2132-2138.
13. Elsenhans B, Strugala GJ, Schafer SG. Small intestinal absorption of cadmium and significance of mucosal metallothionein. Human Exp Toxicol 1997; 16: 429-434.
14. Danielson KG, Ohi S, Huang PC. Immunochemical detection of metallothionein in specific epithelial cells of rat organs. Proc Natl Acad Sci 1982; 79: 2301-2304.
15. Singh R, Rana SVS. Influence of antioxidants on metallothionein-mediated protection in cadmium–fed rats. Biol Trace Elem Res 2002; 88: 71-77.
16. Rana SV, Verma S. Protective effects of GSH, vitamin E, and selenium on lipid peroxidation in cadmium-fed rats. Biol Trace Elem Res 1996; 51: 161-168.
17. Wade MG, Parent S, Finnson KW, Foster W, Younglai E, McMahon A, Cyr DG, Hughes C. Thyroid toxicity due to subchronic exposure to a complex mixture of 16 organochlorines, lead, and cadmium. Toxicol Sci 2002; 67: 207-218.
18. Gupta P, Kar A. Role of ascorbic acid in cadmium-induced thyroid dysfunction and lipid peroxidation. J Appl Toxicol 1998; 18: 317-320.
19. Tsujikawa K, Imai T, Kakutani M et al. Localization of metallothioneinin nuclei of growing primary cultured adult rat hepatocytes. FEBS Lett 1991; 283: 239-242.
20. Hazen-Martin DJ, Sens DA, Blackburn JG, Annsens M. Cadmium nephrotoxicity in human proximal tubule cell cultures. In Vitro Cellular Developmental Biol 1989; 25: 784-790.
21. Goyer RA, Miller CR, Zhu S, Victery W. Non-metallothionein-bound cadmium in the pathogenesis of cadmium nephrotoxicity in the rat. Toxicol Applied Pharmacol 1989; 101: 232-244.
22. Lindh U, Danersund A, Lindvall A. Selenium protection against toxicity from cadmium and mercury studied at the cellular level. Cell Mol Biol 1996; 42: 39-48.
23. Shiraishi N, Uno H, Waalkes MP. Effect of L-ascorbic acid pretreatment on cadmium toxicity in the male Fischer (F344/NCr) rat. Toxicology 1993; 85: 85-100.
24. Metcalfe DD, Baram D, Mekori YA. Mast cells. Physiol Rev 1997; 77: 1033.
25. Wolfreys K, Oliveira DB. Alterations in intracellular reactive oxygen species generation and redox potential modulate mast cell function. Eur J Immunol 1997; 27: 297-306.
Teşekkür: Bu çalışma İstanbul Üniversitesi Rektörlüğü Bilimsel Araştıma Projeleri Yürütücü Sekreterliği tarafın-dan (proje no: 1601/30042001) desteklenmiştir.

Thank you for copying data from http://www.arastirmax.com