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Wistar Ratlarınd a (Rattus rattus norvegicus) Kalitatif ve Kantitatif Protein Yetersizliklerinin İskelet Kası Malondialdehit Düzeylerine Etkileri

Journal Name:

  • Cumhuriyet Üniversitesi Fen Fakültesi Fen Bilimleri Dergisi

Publication Year:

  • 2002

Key Words:

Keywords (Original Language):

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Abstract (2. Language): 
In this study 41 male Wistar rats (Rattus rattus norvegicus) were used. Animals were divided into three groups and were fed eather with a rat chow diet (n=7, controlgroup) or with a semisynthetic diet containing %20 gelatine as protein source (n=17, gelatin group) and no protein (n=17, N-free group). At the end of experiment animals were euthanized, approximately 1 g skeletal muscle samples were taken and MDA levels per gram of muscle tissue were determined. Tissue MDA levels of the rats fed gelatin-containing diet were lower than the controls and animals fed with N-free diet. However, the differences in mean MDA levels among groups were not confirmed statisticcaly. It was concluded that this may be the result of metabolic adaptation processes of organismus to severely protein malnutrition conditions.
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Abstract (Original Language): 
Çalışmada 41 adet yaklaşık 2.5 aylık erkek Wistar ratı (Rattus rattus norvegicus) kullanıldı. Hayvanlar rat yetiştirme yemi (n=7, kontrol), jelatin içeren (n=17) ve protein içermeyen (n=17) yemlerle 35 gün beslendiler. Deney sonunda hayvanlar ötenazi edilerek yaklaşık 1 g kadar iskelet kası örneği alındı ve gram kas dokusu için malondialdehit (MDA) düzeyleri belirlendi. Jelatinle beslenen grupta iskelet kası MDA değerleri kontrol grubu ve N-free diyetle beslenen hayvanlarınkinden daha düşüktü. Ancak gruplar arasında fark istatistiksel olarak onaylanmadı. Bu durumun organizmanın ileri düzeydeki protein yetmezliklerine gösterdiği metabolik uyuma bağlı olduğu düşünülmektedir.
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REFERENCES

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