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Absisik Asit ve Prolin Uygulamasının Laurus nobilis L. Fidelerinde Antioksidant Enzim Aktivitelerinin ve Kuraklığa Tolerans Tepkilerinin Uyarılması Üzerindeki Rolü

Role o f Abscisic Acid and Proline Treatment on Induction of Antioxidant Enzyme Activities and Drought Tolerance Responses of Laurus nobilis L. Seedlings

Journal Name:

  • Cumhuriyet Üniversitesi Fen Fakültesi Fen Bilimleri Dergisi

Publication Year:

  • 2007

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The purpose of this study was to investigate exhibited physiological and biochemical drought tolerance responses of Laurus nobilis L. seedlings by abscisic acid (ABA) and proline treatment to dormant seeds. Abscisic acid and proline was applied to the dormant seeds by imbibitions in sterile water containing 100uM ABA, 1mM proline and ABA + proline combination and maintained at +4°C for 3 weeks. Seedlings were obtained from 4 week stratified seeds sown in pots and were watered regularly during six month. In the leaves of seedlings, the highest activities of superoxide dismutase and catalase were observed in ABA treated and the highest peroxidase activity was measured in the leaves of ABA + proline combination treated group. ABA caused induction of drought tolerance responses was determined by enhancement of paraquat tolerance, proline content, and decreasing of leaf area compared to control. The ABA treatment to dormant seeds can be use for providing drought tolerance ability to seedlings of laurel and its negative effect on growth can be reversed by proline treatment in combination with ABA.
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Abstract (Original Language): 
Bu çalışmanın amacı, Laurus nobilis L. tohumlarına absisik asit (ABA) ve prolin uygulanması ile fidelerde beliren fizyolojik ve biyokimyasal kuraklığa tolerans tepkilerinin araştırılmasıdır. Absisik asit ve prolin uygulaması, tohumların 100uM ABA, 1mM prolin ve ABA + prolin kombinasyonu içeren steril su içerisinde +4°C'da 3 hafta süreyle tutulmasıyla yapılmıştır. Dört hafta katlanmış olan tohumlardan elde edilen fideler saksılara alınarak 6 ay süresince düzenli olarak sulanmıştır. En yüksek süperoksid dismutaz ve katalaz aktivitesi ABA uygulanan grupta, en yüksek peroksidaz aktivitesi ise ABA + prolin kombinasyonunun uygulandığı grubun yapraklarında belirlenmiştir. Absisik asitin kuraklığa tolerans tepkilerini uyarma etkisi; kontrole göre, paraquat toleransı ve prolin içeriğinin artması ile yaprak alanın azalması olarak belirlenmiştir. Tohumlara ABA uygulanması, defne fidelerin kuraklığa toleransının artırılması için kullanılabilir ve uygulamanın büyüme üzerindeki negatif etkisi prolinin ABA ile kombine kullanımıyla giderilebilir.
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  • 1
14-27
  • Turkish

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