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Beta Talasemi Minörlü Hastalarda Eser Element ve Oksidatif Hasar ilişkisi

Relationship Between Trace Element and Oxidative Damage in Patients with Beta-Thalassemia Minor

Journal Name:

  • Fırat Tıp Dergisi

Publication Year:

  • 2009

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: The aim of the present study was to determine levels of reducted glutathione, activities of catalase and superoxide dismutase, levels of malondialdehyde, concentrations of zinc, copper and iron in patients with beta-thalassemia minor (BTM) compared with healthy subjects, and to evaluate the relationships among these parameters. Materials and Methods: The patients consisted of 35 patients with BTM. The control group consisted of 40 healthy subjects. SOD, CAT, GSH and MDA were measured by biochemical methods. Zn, Cu and Fe levels were determined by flame atomic absorption spectrophotometer. Results: SOD and CAT activities, GSH levels, levels of Fe and Zn, hematocrit, hemoglobin and erythrocyte count in the beta-thalassemia minor subjects were found lower than those in control group (p<0.001). However, MDA and Cu levels were significantly higher in BTM subjects than those in the controls (p<0.001). There were significant positive correlations between MDA levels and Cu levels of BTM subjects. However, there were significant negative correlations between GSH and Fe levels. Moreover, there were significant negative correlations between plasma MDA and hematocrit and hemoglobin and erythrocyte count. There were also significant negative correlations between Cu and hematocrit and erythrocyte count. Conclusion: These findings emphasize the significant deficiencies of antioxidant system, Zn and Fe levels and the significant elevation of MDA and Cu levels in patients with BTM. Therefore, supplementation with trace elements involved in the antioxidative prosses may increase scavenger enzyme activities, and consequently, an improvement in clinical symptoms may be expected.
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Abstract (Original Language): 
Amaç: Çalışmanın amacı beta talasemi minörlü (BTM) hasta ve sağlıklı kontrol gruplarında eritrosit redükte glutatyon düzeylerinin, katalaz ve süperoksid dismutaz aktivitelerinin, plazma ve eritrosit malondialdehid düzeylerinin, serum çinko, bakır ve demir konsantrasyonlarının ölçümü, bu parametrelerin birbirleri ile olan ilişkileri her iki grupta karşılaştırmaktır. Gereç ve Yöntem: BTM olan 35 kişilik hasta grubu ile 40 kişilik normal sağlıklı bireyden oluşan kontrol grubunda eritrosit SOD, CAT aktiviteleri, GSH, MDA ve plazma MDA düzeyleri biyokimyasal yöntemlerle ölçüldü. Serum Fe, Cu ve Zn alev atomik absorpsiyon spektrofotometresi ile ölçüldü. Bulgular: Eritrosit SOD ve CAT aktiviteleri, GSH, serum Fe ve Zn düzeyleri, hematokrit, hemoglobin ve eritrosit sayısı hasta grubunda kontrol grubuna göre anlamlı olarak düşük bulundu (p<0.001). Ancak plazma ve eritrosit MDA, serum Cu düzeyleri hasta grubunda kontrollere göre anlamlı olarak yükseldiği görüldü (p<0.001). BTM hasta grubunda plazma MDA ile serum Cu düzeyleri arasında pozitif anlamlı bir ilişki saptandı. Ancak eritrosit GSH ile serum Fe değerleri arasında negatif anlamlı bir ilişki saptandı. Plazma MDA değerleri ile Hct, hemoglobin ve eritrosit sayısı arasında da negatif anlamlı bir ilişki görüldü. Serum Cu konsantrasyonları ile Hct ve eritrosit sayısı arasında negatif bir korelasyon olduğu saptandı. Sonuç: Bulgular BTM'lü hastalarda anlamlı olarak Zn ve Fe düzeyleri ile antioksidan sistem yetersizliğini, MDA ve Cu düzeylerinde artışı göstermektedir. Eser element takviyeli antioksidatif süreç, temizleyici enzim aktivitelerini arttırabilir ve klinik belirtilerinde de iyileşme beklenebilir.
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  • 1
28-32
  • Turkish

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