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AgNOR Yöntemi ile İnsan Epidermisinden Yaş Tespiti

Age Estimation from Human Skins by Using AgNOR

Journal Name:

  • Fırat Tıp Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: The age estimation of postmortem diagnosis is a considerable problem in forensic issues. For age estimation, numerical methods that are evaluated according to proliferative activity of normal cells are tested. AgNOR was exceptionally applied for estimation of chronological ages of unidentified individuals in this study. Use of this method for forensic purposes is presented as an alternative method to determine the age of an individual. Some difficulties seen in the other methods used in routines tried to overcome. Method: In this study, mitotic activity was examined by AgNOR method that was based on silver-staining of nucleolus organizer regions (NORs), and avarage proliferative index was obtained. These index datas were related eachother according to age groups. Results: The study was conducted on 38 postmortem samples (23 males and 15 females) without any tumoral pathology in their past. Samples were divided into four age groups; which were newborns (0-12 months), infants (ages of 1-5), adults (ages of 25-35), and olds (ages of 50 and above). When a brief investigation was performed with AgNOR staining, age-related changes were certainly determined and were found a statistically significant correlation (p<0.01) in AgNOR. Conclusion: In conclusion, although satisfaction in results was obtained for AgNOR, there was a need for further studies involving more individuals. Especially when the other morphological methods are of limited usefulness, AgNOR might be certainly a useful marker in estimating the age of human skin. ©2004, Fırat Üniversitesi, Tıp Fakültesi
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Abstract (Original Language): 
Amaç: Özellikle kimlik tespiti yapılamamış postmortem örneklerden, yaş tespiti yapılması adli tıp için henüz netlik ve pratik uygulamada kolaylık gösterememiş, oldukça önemli bir problem olmaya devam etmektedir. Bu amaçla normal hücrelerin proliferatif aktivitesine göre değerlendirilen kantitatif metodlar denenmektedir. Biz de çalışmamızda AgNOR boyama yöntemi ile insan epidermisinden alınan küçük doku örnekleri ile özellikle kimliği belirlenememiş postmortem olgularda yaş tespitinin yapılabilirliğini göstermeyi amaçladık. Gereç ve Yöntem: Bu çalışmada mitotik aktivite, nükleolar organize edici bölgelerin gümüşle boyanması esasına dayalı AgNOR yöntemiyle incelendi ve ortalama proliferatif indeks elde edildi. Bu indekse bağlı AgNOR dağılımları skoru yaş gruplarına göre ilişkilendirildi. Bulgular: Çalışmaya geçmişinde herhangi bir tümoral hastalık hikayesi olmadığı öğrenilen postmortem örneklerin toplandığı, yeni doğan (0-12 ay), infant (1-5 yaş), erişkin (25-35 yaş), 50 yaş ve üzeri olmak üzere 23 Erkek (E), 15 Kadın (K) toplam 38 olgudan 4 yaş grubu oluşturuldu. Yaş gruplarına göre AgNOR dağılım skorları arasında fark saptandı ve istatistiksel olarak anlamlı korelasyon bulundu (p<0.01). Sonuç: Bu çalışma ile postmortem küçük dokulardan AgNOR boyama yöntemi ile yaş tespiti yapılabileceği, ancak sağlıklı bir değerlendirme için daha geniş vaka serilerine ihtiyaç olduğu sonucuna varıldı. ©2004, Fırat Üniversitesi, Tıp Fakültesi
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  • 3
75-78
  • Turkish

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