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Unilateral testis torsiyonu yapılan ratlarda karşı testiste prednizolon tedavisinin etkileri ve oluşan histopatolojik değişiklikler

Histopathological changes and the effects of prednisolone treatment in contralateral testis in rats subjected to unilateral testicular torsion

Journal Name:

  • Gülhane Tıp Dergisi

Publication Year:

  • 2007

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Abstract (2. Language): 
We aimed to determine the effects of unilateral testicular torsion on the contralateral testis and to evaluate different treatment approaches. Forty pubertal Sprague Downey rats were randomized into 8 groups (Five in each group). In the control group (Group 1), the sham operation was performed. In Group 2, the left testis underwent torsion and was returned to the scrotum. In the other groups, the left testis underwent torsion at 720o counterclockwise and was fixed to the scrotum wall. Then the surgical procedures like detorsion (Group 3, 24 hours later) and orchiectomy (Group 4, 24 hours later and Group 5, 48 hours later); intraperitoneal prednisolone injection in addition to orchiectomy (Group 6, 24 hours later and group 8, 48 hours later), and intraperitoneal prednisolone injection in addition to detorsion (Group 7, 24 hours later) were performed. All the contralateral testes were removed 4 weeks after the torsion and evaluated histopathologically. We observed degeneration at varying intensities in contralateral testes of all groups except for the control group. We found a significant difference in the diameters of seminiferous tubules of the contralateral testes in Groups 2, 3, 4, 5 and 8 compared with the control group. In Groups 6 and 7, no significant changes were observed in the diameters of seminiferous tubules of the contralateral testes, and hyperemia, edema and degeneration were less prominent. We demonstrated that the histopathological damage can be prevented by prednisolone even in the detorsioned group by suppressing the immunologic response.
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Abstract (Original Language): 
Bu çalışmada unilateral testis torsiyonunun kontralateral testise olan etkilerinin araştırılması ve değişik tedavi yaklaşımlarının değerlendirilmesi amaçlanmıştır. Bu amaçla pubertal (yaklaşık 2.5- 3 aylık) 40 adet Sprague-Downey sıçan rastlantısal olarak 5'erli 8 gruba ayrıldı. Kontrol grubunda (Grup 1) testislere yalancı operasyon uygulandı. Grup 2'de sol testis torsiyone edilip bırakıldı. Diğer gruplarda sol testisler 720º saat yelkovanı tersine torsiyone edilip tespit edildi. Daha sonra bu testise detorsiyon (Grup 3'de 24 saat sonra), orşiyektomi (Grup 4'de 24 saat sonra ve Grup 5'de 48 saat sonra) gibi cerrahi müdahaleler ile bazı gruplarda orşiyektomi (Grup 6'da 24 saat sonra ve Grup 8'de 48 saat sonra) ve detorsiyona (Grup 7'de 24 saat sonra) ilaveten intraperitoneal kortizon uygulandı. Tüm kontralateral testisler torsiyondan 4 hafta sonra orşiyektomi ile alınarak histopatolojik değerlendirmeleri yapıldı. Histopatojik olarak kontrol grubu dışında tüm kontralateral testislerde değişen şiddetlerde dejenerasyon mevcuttu. Seminiferöz tübül çapları arasındaki fark, Grup 6 ve Grup 7 dışında önemli bulundu. Bu iki grupta kontralateral hiperemi, ödem ve dejenerasyon da belirgin olarak azdı. Bu bulguların eşliğinde, prednizolon ile histopatolojik hasarın detorsiyone grup da dahil olmak üzere immunolojik cevabın baskılanması yoluyla önlenebileceğini söyleyebiliriz.
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  • 2
67-71
  • English

REFERENCES

References: 

r
damage confirming that the duration of torsion is the most important factor that determines the
degree of contralateral damage.
On the other hand, in some
experimental animal studies, no
damage to the contralateral testis
was reported following unilateral
testicular torsion with no changes
in the semen parameters (1,15). In
some publications, the immunological damage in the contralateral
testis after the unilateral torsion
were not also supported (1,17,18).
Nagler et al. studied the effects of
testicular torsion in prepubertal
rats (19). They found no damage
in the contralateral testis and concluded that prepubertal testicular
torsion may not have the same
implications as postpubertal torsion since the antisperm antibodies which cause immunologic
damage to the contralateral testis
do not appear before puberty.
Cosentino et al. have also worked
with prepubertal rats, and found
that the decrease in spermatogenesis following torsion is more
prominent in the contralateral
testis of pubertal rats compared to
the younger ones (20).
We conclude that the extent of
histological damage to the contralateral testis is dependent on the
duration of the torsion and the
treatment approach. These changes are observed less intensively in
animals that undergo orchiectomy, showing that the presence of
torsioned testis might be responsible for the contralateral damage
mediated by immunologic mechanisms. However, we have demonstrated that the histopathological damage can be prevented
by prednisolone even in the
detorsioned group by suppressing
the immunologic response.
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