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Göbek kordonu kanındaki kök hücre, lenfosit ve lenfosit alt gruplarının immünfenotipik olarak belirlenmesi

Immunophenotypic detection of umbilical cord blood stem cell, lymphocyte and lymphocyte subpopulations

Journal Name:

  • Gülhane Tıp Dergisi

Publication Year:

  • 2008

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Keywords (Original Language):

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Abstract (2. Language): 
In this study it was aimed to differentiate stem cell, lymphocyte and lymphocyte subgroups in umbilical cord blood according to antigenic phenotypes and to determine the relative and absolute counts of these cells. Umbilical cord blood samples were collected from a total of 33 term newborns, 16 female (48.5%) and 17 male (51.5%), all of whom were born by spontaneous vaginal route without any complication during gestation or delivery. Lymphocyte and lymphocyte subgroups and CD34 stem cell ratios were detected by flow cytometry. Umbilical cord blood samples were compared with peripheral venous blood of 33 healthy adults and with bone marrow samples of 23 healthy adults regarding lymphocyte and lymphocyte subgroups, and CD34 stem cell, respectively. In umbilical cord blood, ratios of T cell, cytotoxic T cell and large granular lymphocytes were lower, and total B cell and T helper/suppressor ratios were higher in comparison to peripheral venous blood samples of adults (p<0.05). Numbers of all lymphocyte subgroups except for large granular lymphocytes were higher than those of the healthy adult samples because of the high number of leukocytes in umbilical cord blood (p<0.001). Ratio of CD34 stem cells in umbilical cord blood samples was higher than that of the samples of adult bone marrow (p<0.05). Significant absolute and relative differences were detected between the lymphocyte and lymphocyte subgroups of umbilical cord blood and peripheral adult venous blood, and umbilical cord stem cells and adult bone marrow
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Abstract (Original Language): 
Bu çalışmada yenidoğan bebeklerin kordon kanlarındaki kök hücre, lenfosit ve lenfosit alt gruplarının antijenik fenotiplerine göre ayrımını yapmak ve bu hücrelerin göreceli ve mutlak sayılarını belirlemek amaçlanmıştır. Gebelik, travay ve do- ğum sırasında komplikasyon gözlenmeyen, normal vajinal yol ile dünyaya gelen, 16’sı kız (%48.5), 17’si erkek (%51.5) toplam 33 zamanında doğan yenidoğan bebekten kordon kanı örnekleri alındı. Lenfosit, lenfosit alt grupları ve CD34+ kök hücre oranları akım sitometrisi ile saptandı. Kordon kanı örnekleri, lenfosit ve lenfosit alt grupları açısından 33 sağlıklı erişkin çevre venöz kan örneğiyle; CD34+ kök hücre yönünden ise 23 sağlıklı erişkin kemik iliği örneğiyle karşılaştırıldı. Kordon kanında T hücre, sitotoksik T hücre ve büyük granüllü lenfosit oranları erişkin çevre kanına göre daha düşük; total B hücre ve T hücre helper/suppressor oranları ise daha yüksek saptandı (p<0.05). Kordon kanı beyaz küre sayılarının yüksekliğinden dolayı, büyük granüllü lenfositler hariç bütün lenfosit alt grup sayıları, erişkin örneklerine oranla daha yüksek saptandı (p<0.001). Kordon kanı örneklerinde CD34+ kök hücre oranı, erişkin kemik iliği örneklerine oranla daha yüksek değerde saptandı (p<0.05). Göbek kordonu kanı lenfosit ve lenfosit alt grupları, erişkin çevre venöz kanıyla, kordon kanı kök hücreleri ise erişkin kemik iliğiyle sayı ve orantısal olarak farklılıklar içermektedir.
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  • 4
261-266
  • Turkish

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