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Üst solunum yolu enfeksiyonu yakınmaları olan ilköğretim çağı çocuklarında polimeraz zincir reaksiyonu yöntemi kullanılarak adenovirus araştırılması

Investigation of adenovirus in primary school-age children with the symptoms of upper respiratory tract infection using polymerase chain reaction

Journal Name:

  • Gülhane Tıp Dergisi

Publication Year:

  • 2009

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Abstract (2. Language): 
Adenoviruses, being the common cause of common cold are the viruses which affect many systems and result in various disease presentations in the human and are common in the worldwide. The aim of this study was to detect nasopharyngeal adenoviruses and perform subtype identification of virus isolates in primary school-age children. Throat swab samples were collected from the primary school-age children with the symptoms of respiratory tract infection without an epidemic. Polymerase chain reaction with common adenovirus primers was used to detect adenoviruses. Subgroup identification using conventional polymerase chain reaction with group specific primers was made in samples detected positive for adenovirus by polymerase chain reaction. Afterwards whether the products of polymerase chain reaction were specific or not was confirmed by using MspI restriction enzyme. Of five hundred specimens, 11 (2.2%) were positive for adenovirus. All these 11 specimens were positive with subgroup E (serotype 4) specific primers and negative with subgroup B and C specific primers. Subgroup specific polymerase chain reaction products were positive for subgroup E. Infections caused by adenoviruses are usually seen in the early years of life, and limited number of studies in our country have been performed on pre-school children and students. In this study, all the adenovirus strains were identified as subgroup E (serotype 4). However, data on viral respiratory infections in primary school-age children in Turkey are still unknown, and further studies in larger populations are needed on this subject.
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Abstract (Original Language): 
Sıklıkla soğuk algınlığına neden olan adenoviruslar insanlarda pek çok sistemi etkileyen ve çeşitli hastalık tablolarına yol açabilen, dünya genelinde yaygın virüslerdir. Bu çalışmanın amacı, ilkokul çocuklarında nazofarengeal adenovirusları saptamak ve virüslerin alt tip tanımlamasını yapmaktı. Bir salgın olmaksızın solunum yolu enfeksiyon belirtileri olan ilköğretim çağı çocuklarından boğaz sürüntü örnekleri toplandı. Adenovirusların saptanması için genel adenovirus primerleriyle polimeraz zincir reaksiyonu kullanıldı. Polimeraz zincir reaksiyonu ile adenovirus pozitifliği saptanan örneklerin gruba özgü primerlerin kullanıldığı konvansiyonel polimeraz zincir reaksiyonu yöntemiyle alt grup tanımlaması yapıldı. Bundan sonra polimeraz zincir reaksiyonu ürünlerinin özgül olup olmadığı MspI restriksiyon enzimi kullanılarak doğrulandı. Beş yüz örneğin 11’inde (%2.2) adenovirus pozitif bulundu. Bu örneklerin tümü E alt grubuna (Serotip 4) özgü primerlerle pozitif, B ve C alt gruplarına özgü primerlerle negatif bulundu. Alt gruba özgü polimeraz zincir reaksiyonu ürünleri E alt grubu ile uyumlu bulundu. Adenoviruslara bağlı enfeksiyonlar sıklıkla hayatın erken dönemlerinde görüldüğü için, ülkemizdeki kısıtlı sayıdaki çalışmalar daha çok okul öncesi çocuklar ve öğrencilerle ilişkilidir. Bu çalış- mada tüm adenovirus suşları E alt grubu (serotip 4) olarak bulundu. Ancak Türkiye’de ilköğretim çağı çocuklarında viral solunum yolu enfeksiyonlarına ait veriler hala bilinmemektedir ve bu konuda daha geniş popülasyonlarla yapılacak çalışmalara gereksinim vardır
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PDF icon arastirmax-ust-solunum-yolu-enfeksiyonu-yakinmalari-olan-ilkogretim-cagi-cocuklarinda-polimeraz-zincir-reaksiyonu-yontemi-kullanilarak-adenovirus-arastirilmasi.pdf
  • 3
162-167
  • Turkish

REFERENCES

References: 

Kaynaklar
1. Günaydın M. Adenovirüsler. In: Başustaoğlu A, Kubar
A, Yıldıran ŞT, Tanyüksel M. (çeviri eds). Klinik
Mikrobiyoloji. 9ncu baskı. Ankara: Atlas Kitapçılık,
2009: 1589-1600.
2. Jong JCD, Wermenbol AG, Uijterwaal MWV, et al.
Adenoviruses from human immunodeficiency virus
infected individuals, including two strains that
represent new candidate serotypes Ad50 and Ad51 of
species B and D. J Clin Microbiol 1999; 37: 3940-3945.
3. Hashido M, Mukouyama A, Sakae K, et al. Molecular
and serological characterization of adenovirus genome
type 7h isolated in Japan. Epidemiol Infect 1999; 122:
281-286.
4. Aydin K, Caylan ÖR. Soğuk Algınlığı. In: Uzun Ö, Ünal
S (eds). Güncel Bilgiler Işığında İnfeksiyon Hastalıkları.
Ankara: Bilimsel Tıp Yayınevi, 2001: 161-166.
5. Swenson PD, Wadell G, Allard A, Hierholzer JC.
Adenoviruses. In: Murray PR, Baron EJ, Pfaller MA,
Tenover FC, Yolken RH (eds). Manual of Clinical
Microbiology. 8th ed. Washington DC: ASM Press,
2003: 1404-1417.
6. Inagawa WS, Tanaka K, Uchio E, Itoh N, Ohno S, Aoki
K. Genome typing of adenovirus type 34 isolated in
two cases of conjunctivitis in Sapporo, Japan. J Clin
Microbiol 2001; 39: 4187-4189.
7. Inagawa WS, Oshima A, Aoki K, et al. Rapid diagnosis
of adenoviral conjunctivitis by PCR and restriction
fragment length polymorphism analysis. J Clin
Microbiol 1996; 34: 2113-2116.
8. Ishiko H, Shimada Y, Konno T, et al. Novel human
adenovirus causing nosocomial infections of epidemic
keratoconjunctivitis. J Clin Microbiol 2008; 46:
2002-2008.
9. Sener K, Yapar M, Guney C, et al. Investigation
of the presence and subgroups of adenoviruses in
nasopharyngeal samples of military recruits with
respiratory tract infections. Mikrobiyol Bul 2009; 43:
91-101.
10. Yapar M, Aydogan H, Pahsa A, et al. Rapid and
quantitative detection of Crimean-Congo Hemorragic
Fever Virus by one step real-time reverse transcriptase
PCR. Jpn J Infect Dis 2005; 58: 358-362.
11. Erişim: http://www.ncbi.nlm.nih.gov/Genbank/index.
html. Son erişim tarihi: 16.03.2005.
12. Emel Bozkaya. Solunum sistemi infeksiyonları ve
influenza. In: Ustaçelebi Ş, Abacıoğlu H, Badur S (eds).
Moleküler, Klinik ve Tanısal Viroloji. Ankara: Güneş
Kitabevi, 2004: 101-111.
13. Kim RM, Lee RH, Lee MG. Epidemiology of acute viral
respiratory tract infections in Korean children. J Infect
2000; 41: 152-158.
14. Carballal G, Videla C, Misirlian A, Requeijo PV, Aguilar
CM. Adenovirus type 7 associated with severe and fatal
acute lower respiratory infections in Argentine children.
BMC Pediatr 2002; 2: 6.
15. Aslan SS, Yılmaz G. Akut solunum yolu enfeksiyonlu
çocuklarda adenovirus enfeksiyonu insidansının
saptanması. Türk Mikrobiyol Cem Derg 2001; 31:
242-244.
16. Tsutsumi H, Ouchi K, Ohsaki M, et al.
Immunochromatography test for rapid diagnosis of
adenovirus respiratory tract infections: comparison
with virus isolation in tissue culture. J Clin Microbiol
1999; 37: 2007-2009.
17. Fujimoto T, Okafuji T, Okafuji T, et al. Evaluation of a
bedside immunochromatografhic test for detection of
adenovirus in respiratory samples, by comparison to
virus isolation, PCR and real-time PCR. J Clin Microbiol
2004; 42: 5489-5492.
18. Chen LH, Chiou SS, Hsiao PH, et al. Respiratory
adenoviral infections in children: a study of hospitalized
cases in southern Taiwan in 2001-2002. J Trop Pediatr
2004; 50: 279-284.
19. Allard A, Girones R, Juto P, Wadell G. Polymerase chain
reaction for detection of adenoviruses in stool samples.
J Clin Microbiol 1990; 29: 2683.
20. Echavarria M, Kolavic SA, Cersovsky S, et al. Detection of
adenoviruses (AdV) in culture-negative environmental
samples by PCR during an AdV-associated respiratory
disease outbreak. J Clin Microbiol 2000; 38: 2982-2984.
21. Shike H, Shimizu C, Kanegaye J, Foley J, Burns JC.
Quantitation of adenovirus genome during acute
infection in normal children. Pediatr Infect Dis J 2005;
24: 29-33.
22. Xu WH, Erdman DD. Type-specific identification of
human Adenovirus 3, 7 and 21 by a multiplex PCR
assay. J Med Virol 2001; 64: 537-542.
23. Garnett CT, Erdman D, Xu W, Gooding LR. Prevalence
and quantitation of species C adenovirus DNA in human
mucosal lymphocytes. J Virol 2002; 76: 10608-10616.
24. Harrington KP, Khanna M, Waters CR, Henrickson
KJ. Rapid detection and identification of human
adenovirus species by adenoplex, a multiplex, PCR
enzyme hybridization assay. J Clin Microbiol 2004; 42:
4072-4076.
25. Kinchington PR, Romanowski EG, Jerold Gordon
Y. Prospects for adenovirus antivirals. J Antimicrob
Chemother 2005; 55: 424-429.
26. Echavarria M, Forman M, Ticehurst J, Dumler JS, Carache
P. PCR method for detection of adenovirus in urine of
healthy and human immunodeficiency virus-infected
individuals. J Clin Microbiol 1998; 36: 3223-3226.Cilt 51 • Sayı 3 Çocuklarda adenovirus araştırılması • 167
27. Cooper RJ, Yeo AC, Bailey AS, Tullo AB. Adenovirus
polymerase chain reaction assay for rapid diagnosis of
conjunctivitis. Invest Ophthalmol Vis Sci 1999; 40: 90-95.
28. Echavarria M. Adenoviruses. In: Zuckerman AJ,
Banatvala JE, Schoub BD, Griffiths PD, Mortimer P (eds).
Principles and Practice of Clinical Virology. 6th edition.
Oxford, UK: John Wiley & Sons Ltd., 2009: 463-488.
29. Yamazhan T. 1995-1996 kış mevsiminde, hücre kültürü
yöntemi ile bölgemizde saptanan adenovirus, solunum
sinsityal virus ve parainfluenza virus enfeksiyonları.
Uzmanlık Tezi, 1998, İzmir.
30. Baskın E. Çocukluk çağında adenovirus enfeksiyonları.
Uzmanlık Tezi, 1992, Sivas.
31. Kaygusuz S, Köksal İ, Aydın K, Çaylan R. Investigation
of atypical bacteria and virus antigens in respiratory
tract infections by use of immunofluorescence method.
Jpn J Infect Dis 2004; 57: 33-36.
32. Pumariega T, Savon C, Mune M, et al. Isolation and
identification of adenovirus in hospitalized children,
under five years, with acute respiratory disease, in
Havana, Cuba. Mem Inst Oswaldo Cruz 2000; 95:
859-862.
33. Murtagh P, Cerqueiro C, Halac A, Avila M, Kajon A.
Adenovirus type 7h respiratory infections: a report of
29 cases of acute lower respiratory disease. Acta Pediatr
1993; 82: 557-561.
34. Chuang YY, Chiu HC, Wong SK, et al. Severe adenovirus
infection in children. J Microbiol Immunol Infect 2003;
36: 37-40.
35. Wong S, Pabbaraju K, Pang XL, Lee BE, Fox JD. Detection
of a broad range of human adenoviruses in respiratory
tract samples using a sensitive multiplex real-time PCR
assay. J Med Virol 2008; 80: 856-865.

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