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Prevalence of Metallo-betalactamases (MBL) producing Pseudomonas aeruginosa in a Tertiary care Hospital

Prevalence of Metallo-betalactamases (MBL) producing Pseudomonas aeruginosa in a Tertiary care Hospital

Journal Name:

  • Indian Journal of Basic & Applied Medical Research

Publication Year:

  • 2012

Key Words:

Author Name
Abstract (2. Language): 
Introduction: Pseudomonas aeruginosa isolates are responsible for outbreaks of nosocomial infection in different parts of the world. These isolates have also been responsible for serious infections such as septicemia and pneumonia. MBL production is significant problem in hospital isolates of Pseudomonas aeruginosa. P aeruginosa is a pathogen associated with numerous nosocomial infections in immunocompromised patients .The present study was conducted at our hospital with an aim to know the prevalence of Carbapenem resistance & production of metallo-betalactamases producing strain of Pseudomonas aeruginosa in our hospital. Material & Method: This study was conducted at our hospital during April 2010 to October 2011.Total 116 strain of Pseudomonas isolated from different samples like pus, swab, Urine, ET secretion etc were evaluated for Carbapenems resistance & MBL production. Various methods has been recommended for screening MBL producing strain like modified Hodge test, EDTA impregnated Imipenem disk & EDTA impregnated Meropenem disk, disk potentiation test with EDTA.We use disk potentiation test with EDTA impregnated Imipenem disk in this study. Conclusion: In our study out of 116 isolates 20(17.2%) isolates are resistant to imipenem & Meropenem. We have confirmed 14(12%) isolates are MBL producers by disk potentiation test. In India prevalence of MBLs range from 7 – 65% with a recent study reporting 34% occurrence. The early detection of MBL producing P aeruginosa may help in appropriate antimicrobial therapy and avoid the development & dissemination of these multidrug resistance strains. So all isolates of P aeruginosa resistant to imipenem should be screened for MBL production. Combined disk diffusion or disk potentiation test should be introduced in every clinical microbiology laboratory in order to aid infection control.
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  • 4
304-308
  • Turkish

REFERENCES

References: 

1) Livermore DM. Has the era of untreatable infection
arrived? J. Antimicrob Chemother 2009;64:29-36
2) Bush K.β lactamases of increasing clinical importance.
Curr Pharm Des 1999:5:839-45
3) Forbes BA, Sham DF, Weissfeld AS. Bailey and Scott’s
diagnostic Microbiology, 12th Ed. New York: 2007: 340-350.
4) Clinical and laboratory standards Institute, Performances
standards for antimicrobial disk test,M100-S16, 2006
5) Hemalatha V, Sekar U, Kamat V. Detection of metallo- β-
lactamase producing Pseudomonas aeruginosa in
hospitalized patients.Indian J Med Res2005: 122:148-52
6)Young D,Lee K, Yum J H, Shin H B, Ressolinism, Chong
Y.Imipenem –EDTA disk method for differentiating of
mettalo- β-lactamase producing clinical isolates of
Pseudomonas spp. And Acinetobacter spp. J Clin Microbiol
2002;40:3798-801.
7) Walsh TR, Toleman MA, Pirel L, Nordmann P. Mettalo- β-
lactamase; the quiet befor the storm? Clin Microbiol Rev 2005:18:306-
25
8)Migliavacca R, Docquire JD, Mugnaioli C, Amicosante G, Daturi R,
Lee K et al. Simple Microdilution test for the detection of mettalo- β-
lactamase production in Pseudomonas aeruginosa. J Clin Microbiol
2002;40:4388-90.
9) Pitout J D, Gregson DB, Poirel L, McClure J A,Le P,Church Dl.
Detection of Pseudomonas aeruginosa producing metallo-betalalactamases
in a large centralized laboratory. J Clin Microbiol
2005:43:3129-35
10) Castanheira M, Bell J M, Turnidge JD, Mathai D, Jones RN.
Carbapenem reisstance among Pseudomonas aeruginosa strains from
India: Evidence for Nationwide endemicity of Multiple Metallo- β-
lactamase Clones(VIM-2,-5,-6 and -11) and the Newly characterized
VIM-18).Antimicrob Agents Chemother 2009; 53:1225-7.
11) Attal RO, Basak S,et al; Mettalobetalactamase producing
Pseudomonas aeruginosa: An emerging threat to clinicians.Journal of
clinical and Diagnostic Resaech,2010 August(4)2691-2696
12) Navneeth B, Shridaran D, Sahay D, Belwadi MRS, A preliminary
study on metallobetalactamases producing of P aeruginosa in
hospitalized patients. Indian J Med Res 2002;116;264-7
13) Mendiratta DK, Deotale V, Narang P, Metallobetalactamases
producing of P aeruginosa in hospital from rural area. Indian J Med Res
2005;121: 701-3
14) Agrawal G, Lodhi RB,Kamalakar UP, Khadse RK, Jalgaokar SV.
Study of metallobetalactamases production in clinical isolates of P
aeuginosa . Indian J Med Microbiol 2008;26(4):349-51.
15)Pitout JD, Laupland KB. Extended spectrum beta-lactamases
producing Enterobacteriaceae: An emerging public health concern.
Lancet infection Dis2008:8:159-66
16) Bonfiglio G, Laksai Y, Franchino L. Amicosante G, Nicoletti G.
Mechanism of β lactam- resisatnce amongst Pseudomonas aeruginosa
isolated in an Italian survey. J Antimicrob Chemother 1998; 42:697-
702
Indian Journal of Basic & Applied Medical Research; September 2012: Vol.-1, Issue-4, P. 304-308
307
www.ijbamr.com
17) Behera B, Mathur P, Das A, Kapil A, Sharma V. An
evaluation of four different phenotyping techniques for
detection of metallo –β-lactamase producing Pseudomonas
aeruginosa. Indian J Med.Microbiol 2008:26:233-7
18) Gales Ac,Menezez LC, Silbert S, Sadar HS. Dissemination
in district Brazilian regions of an epidemic
Carbapenem resistant Pseudomonas aeruginosa producing
SPM metallo-beta-lactamases. J Antimicrob chemother
2003;52:699-702
19)Lee K, Lee VG, Uh Y, Ha GY,Cho J, ChongY, VIM- and
IMP- type metallo-beta-lactamases producing Pseudomonas spp
and Acinetobacter spp. In Korean hospital. Emerg infect Dis
2003;9:868-71
20) Sarma JB, Ahmed GU, infection control with limited
resources: Why & How to make it possible? Indian J Med
Microbiol 2010;28:11-6

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