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PROTEIN CARBONYL & MICROALBUMINURIA IN TYPE 2 DIABETES MELLITUS

PROTEIN CARBONYL & MICROALBUMINURIA IN TYPE 2 DIABETES MELLITUS

Journal Name:

  • Indian Journal of Basic & Applied Medical Research

Publication Year:

  • 2012

Key Words:

Author Name
Abstract (2. Language): 
INTRODUCTION: Diabetes is a devastating disease throughout the world. It is associated with several mechanisms, one of which is oxidative stress. Oxidative stress plays an important role in the pathogenesis and the complications of diabetes. A hyperglycemia results in overproduction of oxygen free radicals, which contributes to the progression of diabetes. The development of complications during diabetes is associated with oxidative stress. Measuring protein carbonyl associated with nephropathy as oxidative protein damage is the primary aim of our study . METHODS: We measured protein carbonyl, urine microalbumin & glycated hemoglobin in type 2 Diabetes mellitus & studied for correlation among these parameters towards development of nephropathy. Fifty diabetic and fifty age matched healthy subjects were included in the study. Both the groups were evaluated for protein carbonyl, urine microalbumin & glycated hemoglobin. OBSERVATIONS: Plasma protein carbonyls were significantly (p< 0.001) elevated in diabetes (0.091 ± 0.13 mmol/L) when compared with healthy subjects (0.037 ± 0.012 mmol/L). Glycated hemoglobin has shown proportional increase with blood glucose. Urine microalbumin is found to be increased in patients with diabetes & positively correlated with protein carbonyl. RESULTS: Increased formation of protein carbonyl, a marker of oxidative stress produced under hyperglycemia of diabetes mellitus may be one of the probable cause for evolution of nephropathy in diabetes mellitus.
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  • 5
399-404
  • Turkish

REFERENCES

References: 

1. Zimmet, P.Z., McCarty, D.J., and de Courten, M.P. The global epidemiology of non-insulin-dependent diabetes mellitus and the metabolic syndrome. Journal of Diabetes and its Complications. 1997; 11: 60-68.
2. Maritim AC, Sanders RA, Watkins JB. Diabetes, oxidative stress, and antioxidants: A Review. J Biochem Mol Toxicol. 2003; 17: 24¬38.
3. Brownlee, M. Biochemistry and molecular cell biology of diabetic complications.
Nature. 2001; 414: 813-20.
4. David S Small, Jeff S Coombes, Nigel Bennett, David W Jhonson, Glenda C Globe. Oxidative stress, anti-oxidant therapies and chronic kidney disease: Review Article. Nephrology; NEP-2011 -0573.R1
Accept Date: 17-Jan-2012
5. Requena, JR., Levine, RL. and Stadtman, ER.Recent advances in the analysis of
oxidized proteins. Amino Acids. 2003; 25: 221-6.
6. Dalle-Donnea, Ranieri Rossib, Daniela Giustarinib , Aldo Milzania, Roberto Colombo Isabella
Review - Protein carbonyl groups as biomarkers of oxidative stress.
Clinica Chimica Acta 2003; 329: 23-38.
Indian Journal of Basic & Applied Medical Research; December 2012: Issue-5, Vol.-2, P. 399-404
7. Ramakrishna V, Jailkhani R: Evaluation of oxidative stress in insulin dependent diabetes mellitus (IDDM) patients. Diagnostic Pathology 2007; 2(22): 1746-1596.
8. Sen S.Kar M, Roy A,Chakraborty A S. Effect of nonenzymatic glycation on functional & structural properties of hemoglobin. Biophysic chemisty 2005; 113: 289-98.
9. Jakus V. The role of free radicals, oxidative stress and antioxidant systems in diabetic vascular disease. BRATISL LEK LISTY 2000; 101 (10): 541- 551
10. Reznick AZ, Packer L.et al: Oxidative damage to proteins: Spectrophotometric method for carbonyl assay. Methods Enzymol 1994; 233: 357- 363.
11. Maiese K, Morhan SD, Chong Z Z: Oxidative stress biology and cell injury during type 1 and type 2 diabetes mellitus. Curr Neurovasc Res 2007; 4(1):63-71.
12. Hawkins CL, Morgan PE, Davies MJ. Quantification of protein modification by oxidants. Free Radic Biol Med.
2009; 46(8):965-88.
13. Levine R.L., Garland D., Oliver C.N., Amici A, Cliement I, Lenz A.G. et al. Determination of carbonyl content in oxidatively modified proteins. Method Enzymol ; 1990: 186: 464-478.
14. Origin Dubourg L, de Precigout V, Aparicio M, Clerk M: Evaluation and significance of "carbonyl stress" in long-term uremic complications. Kidney Int. 1999; 55:389-399.
15. Agnieszka Piwower , Maria Knapik-Kordecka, Maria Warwas: Connection Between Ischemia-Modified Albumin Levels and Markers of Diabetic Nephropathy and Oxidative
Protein Damage in Type 2 Diabetic Patients: Adv Clin Exp Med 2009; 18(4):353-360.
16. U Çakatay.Protein oxidation parameters in type 2 diabetic patients with good and poor glycaemic control. Diabetes & Metabolism. 2005; 31(6) - 551-557.
17. Ryuichi Kikkawa, MD, Daisuke Koya, MD, and Masakazu Haneda, MD: Progression of Diabetic Nephropathy. American Journal of Kidney Diseases, 2003; 41(3): S19-S21.
18. Vicentini, Juliana MS; Valentini, Juliana MS; Grotto, Denise MS; Paniz, Clovis MS; Roehrs, Miguel MS et al. Association among Microalbuminuria and Oxidative Stress Biomarkers in Patients with Type 2 Diabetes. Journal of Investigative Medicine. 2011, 59 ( 4) : 649-654.
19. Rohlfing CL, Wiedmeyer HM, Little RR, England JD, Tennill A, Goldstein DE. Defining the relationship between plasma glucose and HbA(1c): analysis of glucose profiles and HbA(1c) in the Diabetes Control and Complications Trial. Diabetes Care. 2002. 25(2), 275-8.
20. Vivian Fonseca, Silvio E. Inzucchi, and Ele Ferrannini. Redefining the Diagnosis of Diabetes Using Glycated Hemoglobin. Diabetes Care.2009; 32 (7), 1344-1345.
21. Padala Ravi Kumar, Anil Bhansali, Muthuswamy Ravikiran, Shobhit Bhansali,Pinaki Dutta, J. S. Thakur. et al. Utility of Glycated Hemoglobin in Diagnosing Type 2 Diabetes Mellitus: A Community-Based Study. ENDOCRINE CARE; 2010: 95 (6), 2832.
22. Sundaram RK, Bhaskar A, Vijayalingam S, Viswanathan M, Mohan R, Shanmugasundaram KR. Antioxidant status and lipid peroxidation in type II diabetes mellitus with and without complications. Clin Sci (Lond), 1996; 90, 255-60.
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www.ijbamr.com
Indian Journal of Basic & Applied Medical Research; December 2012: Issue-5, Vol.-2, P. 399-404
23. Kanti Bhooshan Pandey and Syed Ibrahim Rizvi : Age-dependent oxidative stress biomarkers in type 2 diabetic patients. Journal of human diseases, 2011; 1: 1-9.

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