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Isolation, Production And Purification Of Streptokinase From Streptococcus Py ogenes

Journal Name:

  • Indian Streams Research Journal

Publication Year:

  • 2013

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Abstract (Original Language): 
Streptokinase a fibrinolytic enzyme is very effective in treating acute myocardial infarction and it is certainly more cost-effective. In view of the relatively recent availability of the competing recombinant tPA, skepticism is being expressed about the continued viability of streptokinase therapy. Despite this research on streptokinase continues, and it remains a vital and affordable therapy especially in the world's poorer healthcare systems. Our present study focuses on the production of streptokinase from Streptococcus pyogenes species and partial purification of streptokinase by ammonium sulfate precipitation, dialysis and column chromatography. The enzyme is quantified by Lowry's method and its electrophoretic mobility and molecular weight were determined by SDS-PAGE.
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REFERENCES

References: 

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Preparation and certain properties highly purified
Indian Streams Research Journal ISSN 2230-7850
Volume-3, Issue-6, July-2013
streptokinase. J Biol Chem. Endrogan E, Ozer AY, Volkan B,
Caner B, Bilgili H (2006). Thrombus
vi.Francis, C.W., Marder, V.J., 1991. Fibrinolytic therapy for
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enolase gene under in vivo
viii.Banerjee A, Chistic Y, Banerjee UC (2004).
Streptokinase, a clinically useful thrombolytic agent.
Biotechnol. Adv.
ix.Baewald G, G. Mayer, R. Heikel, KD. Volzke, R. Roehlig,
KL. Decker. (1975). Fermentative production of
Streptococcus metabolites, especially streptokinase.
German patent DD.
x.Chitte R.R, Dey S (2000). Potent fibrinolytic enzyme from
a thermophilic Streptomyces megasporus strain SD 5. Lett.
Appl. Microbiol.
xi.Collen, D., 1990. Coronary thrombolysis: streptokinase or
recombinant tissue-type plasminogen activator? Ann. Intern.
Med.
xii.De Renzo EC, Siiteri PK Hutchings BL, Bell PH. (1967)
Preparation and certain properties highly purified
streptokinase. J Biol Chem. Endrogan E, Ozer AY, Volkan B,
Caner B, Bilgili H (2006). Thrombus
xiii.Francis, C.W., Marder, V.J., 1991. Fibrinolytic therapy
for venous thrombosis. Prog. Cardiovasc. Dis.

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