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Isolation And Purification Of Plant Growth Promoting Rhizobacteria (Pgpr) From The Rhizosphere Of Acorus Calamus Grown Soil

Journal Name:

  • Indian Streams Research Journal

Publication Year:

  • 2013

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Abstract (Original Language): 
Among the 10 bacterial isolates were obtained from Acorus calamus rhizospheric soil of Melaiyar and Nagapattinam districts in Tamil Nadu. All the isolates were identified as Azospirillum spp., Bacillus spp., Pseudomonas spp., and Azotobacter spp. These bacterial strains were tested on morphological, biochemical and screened for their direct growth promoting activities (IAA production, production of Ammonia and Phosphate solubilization) and indirect growth promoting activities (HCN production, Siderophore production). The results obtained showed that among the 10 isolates of Melaiyar and Nagapattinam districts (M-M15) of ranged from (4.10 - 5.66 and 4.40 - 7.80) of Azospirillum spp., (3.00 – 6.00 and 3.20 – 6,.30 ) of Bacillus spp., (5.00 – 8.40 and 5.10 - 8.20) of Pseudomonas spp., and (3.00 - 6.00 and 3.30 - 6.30) of Azotobacter spp. The IAA production of Pseudomonas spp. (94%), Azospirillum spp (80%), Azotobacter spp. (65%) and Bacillus spp. (40%). Ammonium production of the isolates, Bacillus spp. (96%), Pseudomonas spp. (92%), Azospirillum spp. (65%) and Azotobacter spp. (55%). The siderophore and HCN production produced by all the isolates make it suitable for further investigation of pot and field trials by Acorus calamus cultivation
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