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Zidovudine karşı HIV rezistansının moleküler temeli ve rezistansın polinteraz zincir reaksiyonu ile saptanması

Molecular basis of HIV resistance to zidovudine and its detection by polymerase chain reaction (PCR)

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 1994

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Zidovudine (AZT) therapy decreases- both mortality and the incidence of opportunistic injections in the patients infected with human immunodeficiency virus (I111'). The resistance to AZT. however, is an im/wrtant problem. This resistance is associated with the point mutations at codon 41 (Met to Leu). 6~ (Asp to Asn). ~() (Lys to Arg). 215 (Thr to Phe or Tyr) and 219 (Lys to (ilu) in the reverse transcriptase coding region of ' pot gene. PCR amplification technique is used most commonly to determine these mutations. /.Journal of Turgut Özal Medical Center I(4):321-323.1994/
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Abstract (Original Language): 
Zidovudin ile tedavi, human immunodeficiency virus (HIV') ile infekte hastalarda gözlenen fırsatçı infeksiyonların insidansını ve mor tali teyi azaltmaktadır. Ancak zidovudine karsı direnç gelişimi önemli bir problem oluşturmaktadır. Bu rezistans; H1V pol gen bölgesinde revers transkriptaz enzimini kodlayan alandaki 41 (Met—*Leu) inci, 67 (Asp—>Asn)'inci, "0 (I,ys—>Arg) inci. 215 (Thr—>Phe veya Thy)'inci ve 219 (Lys—>(ilu) 'uncu kolonlarda meydana gelen nokta mutasyonlarla ilgilidir. Bu mutasyonların saptanmasında PCR amplifikasyon tekniği çok yaygın olarak kullanılmaktadır. [Turgut Özal Tıp Merkezi Dergisi l(4):32l-323.1994]
FULL TEXT (PDF): 
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  • 4
321-323
  • Turkish

REFERENCES

References: 

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