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Gebe Kadınlarda Herpes simplex Virus Tip-1 Ve 2, IgG ve IgM Antikorlarının, ELISA ile Araştırılması

Detection of Herpes simplex Virus type-1 and 2 (HSV-1 and HSV-2) IgG and IgM Antibodies with ELISA in Sera of Pregnant Women

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: Detection of Herpes Simplex Virus type-1 and 2 (HSV-1 and HSV-2) IgG and IgM Antibodies with ELISA in the sera of pregnant women. Material and Method: 8-10 ml venous blood were taken from 96 pregnant women in different periods of their pregnancies and centrifuged in Department of Microbiology, Ankara University Faculty of Medicine, Laboratory of serology. The tests were performed in accordance with test guides and the results were read in EL-9 Microplate reader. Results: HSV-1 IgG was positive in 71/96 (%74) women. HSV-1 IgM was positive in only one women. HSV-2 IgG was positive in %62.5 (60/96) and IgM in %11.5 (11/96) women. The HSV-2 IgM seropositivity was seen between 15-30 years of age. Conclusions: In a community the HSV infection before and after the pregnancy is an important health problem especially for infants. Therefore, it is useful to investigate the HSV-1 and 2 antibodies in the pregnanat womens’ sera.
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Abstract (Original Language): 
Amaç: Gebe kadınların serumlarında, kontrol amaçlı olarak, ELİSA ile, Herpes simplex Virus (HSV) tip 1 ve 2 IgG ve IgM antikorlarının araştırılması, Gereç ve Yöntem: Değişik gebelik haftalarında bulunan bir grup gebe kadından, steril koşullarda 8-10 ml venöz kan alınmış ve bekletilmeksizin Ankara Üniversitesi Tıp Fakültesi Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı Seroloji laboratuvarında serumları ayrılmıştır. EL-9 Microplate Reader’de Virotech, Trinity-Biotech, Meddens ve Novum-Diagnostica test kitlerinin önerilerine göre deneyler yapılmıştır. Bulgular: Gebelerde HSV-1 IgG pozitifliği %74 (71/96) bulundu. HSV-1 IgM ise bir olguda pozitif olarak saptandı. HSV-2 IgG %62.5 ve IgM %11.5 oranlarında pozitiflik gösterdi. HSV-2 IgM seropozitifliğinin 15-30 yaşları arasında toplandığı görüldü. Sonuçlar: Bir toplum için gebelik öncesi ve gebelik sonrası HSV infeksiyonları, özellikle bebekler için önemli bir sağlık sorunu oluşturmaktadır. Bu nedenle gebelerde HSV-1 ve 2 göstergelerinin araştırılmasının yararı açıktır.
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