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Diyabet Hastalarının Lens Ön Kapsüllerinde Glutatyon Seviyesi ve Glutatyon Peroksidaz Aktivitesi

Lens Capsular Glutathione Level and Glutathione Peroxidase Activity Among Diabetic Patients

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Purpose: To evaluate the level of glutathione (GSH) and gluthathione peroxidase (GSH-Px) activity in the anterior lens capsule of patients with cataract in diabetic patients with meticulous control and in patients with senile cataract. Method: The anterior lens capsules in 18 eyes with diabetes under tight glycemic control with cataract (Group A) and in 26 eyes with senile cataract (Group B) were collected in phacoemulsification surgery. The specimens were thawed, centrifuged and the supernatants of the homogenate obtained from the samples were analyzed for the GSH level and GSH-Px enzyme activity. Results: Group A composed of 10 female and 8 male patients and mean age of patients was 60.3 ± 8.3 years. Group B composed of 11 female and 15 male patients and mean age of the patients was 63.9 ± 12.2 years. Mean of the measurements for GSH level and GSH-Px activity at anterior capsule were 82.4±16.5 vs. 97.5±12.5 nmol/mg and 27.0±9.7 vs. 36.9±9.3 μmol/mg protein, in group A and B, respectively. The differences between groups were without any statistical significance for both parameters(p>0.05). Conclusion: The detorious effect of diabetes on anti-oxidant mechanisms is a well known subject. Our results indicate that approximate level of GSH and GSH-Px activity between diabetics with tight glucose control group and age-matched control group may be the result of meticulous control of hyperglycemia.
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Abstract (Original Language): 
Amaç: Bu çalışmada yaşa bağlı kataraktı bulunan hastalar ile sıkı kontrol altındaki diabet hastalarının lens ön kapsüllerinde ölçülen glutatyon (GSH) seviyesi ve glutatyon peroksidaz (GSH-Px) aktiviteleri değerlendirilmiştir. Yöntem: Çalışmaya tip II diabeti ve kataraktı bulunan hastalar ile yaş uyumlu olacak şekilde yaşa bağlı kataraktı bulunan hastalar dahil edildiler. Çalışma kapsamında sıkı diabet kontrolünde olan 18 hasta ile (Grup A), yaşa bağlı kataraktı bulunan 26 hastanın (Grup B) fakoemulsifikasyon yöntemi ile yapılan katarakt operasyonlarında alınan lens ön kapsülleri dahil edilmiştir. Örnekler çözünüp, santrifüje edilmelerinden sonra numunelerden GSH seviyeleri ve GSH-Px enzim aktiviteleri ölçülmüştür. Sonuçlar: Grup A ortalama yaşları 60.3 ± 8.3 yıl olan, 10 bayan ve 8 erkek hastadan oluşmaktaydı. Grup B ise ortalama yaşları 63.9 ± 12.2 yıl olan, 11 bayan ve 15 erkek hastadan oluşmaktaydı. Grup A ve B için lens ön kapsülünde ölçülen ortalama GSH seviyeleri sırasıyla 82.4±16.5 ve 97.5±12.5 nmol/mg iken ortalama GSH-Px aktivite düzeyleri sırasıyla 27.0±9.7 ve 36.9±9.3 μmol/mg protein idi. Her iki parametre içinde gruplar arasındaki fark istatistiksel olarak anlamlı düzeyde tespit edilmedi (p>0.05). Yorum: Diabetin anti-oksidan mekanizmalar üzerindeki zararlı etkisi iyi bilinmektedir. Bizim sonuçlarımızda GSH seviyesinin ve GSH-Px aktivitelerinin sıkı kontrollü diabet grubunda ve yaşa bağlı kataraktı grubu arasında yakın değerlerde çıkmış olması, diabetin sıkı kontrolünün diabetin lens üzerindeki etkilerini hafifletmek bakımından önemli olduğunu düşündürtmektedir.
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  • 3
139-143
  • Turkish

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