Buradasınız

Anasayfa » Content

Kutanöz mast hücrelerinden histamin salınımı üzerine ovabain'in etkisi

The Effect of Ouabain on Histamine Release from Human Skin Mast Cells

Journal Name:

  • İnönü Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 1996

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
There are controversial reports on the effect of sodium-potassium adenosine triphosphatase (Na+-K+ ATPase) inhibition on mast cell mediator release. Some of them have indicated that ouabain (strophanthin G), a specific Na+-K+ ATPase inhibitor, inhibited the release, whereas the others have shown that ouabain had no effect or even had a stimulatory effect on the mediator secretion. Most of these studies utilized animal-derived cells. The aim of this study was to determine the effect of Na+-K+ ATPase inhibition on human skin mast cells. Unpurified and purified mast cells were obtained from newborn foreskins and stimulated by calcium ionophore A23187 (1 fiM) for 30 min following a 1 hr incubation with various concentrations (10-4 to 10-8 M) of ouabain. Histamine release was assayed by enzyme immunoassay. The results indicated that ouabain, a Na+-K+ ATPase inhibitor, had no significant effect on the non-immunologic histamine release from human skin mast cells in vitro and suggested differences between human and animal mast cells. [Journal of Turgut Özal Medical Center 1996;3(4):277-283]
Bookmark/Search this post with
Abstract (Original Language): 
Sodyum-potasyum adenozin trifosfataz (Na+-K+ ATPaz) inhibisyonunun, mast hücrelerinden mediatör salınımı üzerine etkileri konusunda çelişkili bilgiler mevcuttur. Bazı çalışmalar, spesifik bir Na+-K+ ATPaz inhibitörü olan ovabain (strofantin G) 'in, mediatör deşarjını bloke ettiğini, bazıları ise herhangi bir etkisinin olmadığını, hatta mediatör sekresyonunu artırdığını ileri sürmektedir. Bu çalışmaların çoğu hayvanlardan elde edilmiş mast hücreleri kullanılarak yapılmıştır. Bu çalışmanın amacı, Na+-K+ ATPaz inhibisyonunun, insan kutanöz mast hücrelerinden mediatör salınımı üzerine etkilerini incelemektir. Sağlıklı yenidoğanların sünnet derilerinden elde edilen mast hücreleri, değişik ovabain konsantrasyonlarvyla inkübe edildikten sonra, kalsiyum iyonoforla stimüle edildi. Histamin salınımı ELISA ile değerlendirildi. Sonuçlar, spesifik bir Na+-K+ ATPaz inhibitörü olan ovabain'in, insan kutanöz mast hücrelerinden in vitro nonimmünolojik histamin salınımı üzerine belirgin bir etkisi olmadığını gösterdi. İnsan ve hayvan mast hücreleri arasında bu açıdan da farklılıklar olduğu düşünüldü. [Turgut Özal Tıp Merkezi Dergisi 1996;3(4):277-283]
FULL TEXT (PDF): 
PDF icon arastirmax-kutanoz-mast-hucrelerinden-histamin-salinimi-uzerine-ovabainin-etkisi.pdf
  • 4
277-283
  • Turkish

REFERENCES

References: 

1. Goldstein SM, Witroub BU. The cellular and molecular biology of the human mast cell. In: Fitzpatrick TB, Eisen AZ, Wolf K, et al, eds. Dermatology in General Medicine. New York: Mc Graw-Hill, 1993: 359-73.
2. Longley J, Duffy TP, Kohn S. The mast cell and mast cell disease. J Am Acad Dermatol 1995; 32: 545-61.
3. Weber S, Krasagakes SK, Grabbe J, et al. Mast cells. Int J Dermatol 1995; 34: 1-10.
4. Inagaki N, Kawasaki H, Ueno M, et al. Potentiation of antigen-induced histamine release from rat peritoneal mast cells through a direct interaction between mast cells and nonmast cells. Life Sciences 1994; 54: 1403-9.
5. Church MK, El-Lati S, Okayama Y. Biological properties of human skin mast cells. Clin Exp Allergy 1991; 21: 1-9.
6. Benyon RC. The human skin mast cell. Clin Exp Allergy 1989; 19: 375-87.
7. Nichols JR, Maldonado HS. Comparison of the inhibitory effect of ouabain and dihydroouabain on the Na+-K+ ATPase from frog skin. Gen Pharmacol 1993; 24: 349-52.
8. Amellal M, Binck M, Frossard N, et al. Sodium-potassium ATPase inhibition potentiates compound 48/80-induced histamine release from mast cells. Br J Pharmacol 1984; 82:
423-30.
9. Okazaki T, Ilea VS, Okazaki A, et al. Inhibition of antigen-induced histamine release by ouabain. J Allergy Clin
Immunol 1976; 57: 454-62.
10. Frossard N, Amellal M, Landry Y. Sodium-potassium ATPase, calcium, and immunological histamine release.
Biochem Pharmacol 1983; 32: 3259-52.
11. Stump RF, Oliver JM, Cragoe EJ, Deanin GG. The control of mediator release from RBL-2H3 cells: Roles for Ca2+,
Na+, and protein kinase C. J Immunol 1987; 139: 881-6.
12. Rumpel E, Pilatus E, Mayer A, Pecht I. Na+ and Ca2+
gradients across the membrane modulate the secretory response of mast cells. Int Arch Allergy Appl Immunol
1995; 107: 351-3.
13. Erjavec F, Ferjan I. Changes in histamine secretion from mast cells caused by digitalis glycosides. Agents Actions
1990; 30: 125-7.
14. Fewtrell C, Mohr C, Ryan TA, Millard PJ. Calcium: an
important second messenger in mast cells. Ciba Foundation
Symposium 1989: 114-32.
15. Diamant P, Patkar SA. Stimulation and inhibition of histamine release from isolated rat mast cells. Int Arch
Allergy Appl Immunol 1975; 49: 183-207.
16. Knudsen T, Ferjan I, Johansen T. Activation of the Na+/K+ pump in rat peritoneal mast cells following histamine release: a possible role in cell recovery. Br J Pharmacol
1993; 108: 120-5.
17. Fewtrell CMS, Gompert BD. Effect of flavone inhibitors of transport ATPase on histamine secretion from rat mast cells. Nature 1977; 265: 635-6.
18. Magro AM. Ethacrynic acid inhibitable Ca2+ and Mg2+ activated membrane adenosine triphosphatase in rat mast cells. Clin Exp Immunol 1977; 30: 160-7.
19. Magro AM. Blocking of histamine release from human basophils in vitro by ATPase inhibitor, ethacrynic acid. Clin
Exp Immunol 29: 436-41, 1977.
20. Smith TF, Legrand FS, McKean LP, et al. Role of sodium in mediator release from human basophils. J Allergy Clin
Immunol 1992; 89: 978-86.
21. Knudsen T, Bertelsen H, Johansen T. Ouabain enhancement of compound 48/80-induced histamine secretion from rat peritoneal mast cells: dependence on extracellular sodium.
Pharmacol Toxicol 1992; 70: 412-8.
22. Knudsen T, Ferjan I, Johansen T. Effect of ouabain, digoxin, and digitoxigenin on potassium uptake and histamine release from rat peritoneal mast cells. FEBS Letters 1993;
321: 127-31.
23. Amellal M, Bronner C, Landry Y. Transmembrane sodium and potassium gradients modulate histamine secretion induced by ionophore A23187. Br J Pharmacol 1985; 85:
819-26.
24. Gentile DA, Skoner DP. A role for Na+-K+ ATPase in
degranulation of RBL-2H3 cells. Clin Exp Allergy 1996: (In press).
25. Gentile DA, Braun E, Skoner DP. The effect of Na+-K+
ATPase inhibition on leukocyte histamine release Annal Allergy Asthma Immunol 1996: (In press).
26. Robinson C, Benyon RC, Holgate S, Church MK. The IgE-and calcium-dependent release of eicosanoids and histamine from human purified cutaneous mast cells. J Invest
Dermatol 1989; 93: 397-404.
27. Lawrence ID, Warner JA, Cohan VL, et al. Purification and characterization of human skin mast cells. J Immunol 1987;
139: 3062-9.
28. Benyon RC, Lowman MA, Church MK. Human skin mast cells: their dispersion, purification, and secretory characterization. J Immunol 1987; 138: 861-7.
29. Tharp MD, Suvunrungsi RT, Sullivan TJ. IgE-mediated release of histamine from human cutaneous mast cells. J Immunol 1983; 130: 1896-901.
30. Siraganian RP. Histamine secretion from mast cells and
basophils. TIPS 1983; 4: 432-7.
31. Morel AM, Delaage MA. Immunoanalysis of histamine through a novel chemical derivatization. J Allergy Clin
Immunol 1988; 82: 646-54.
32. Coligan JE, Kruisbeek AM, Margulies DH. Trypan blue exclusion test of cell viability. Current Protocols in Immunology. New York: John Wiley & Sons, 1991: A.3.3.
33. Thompson HL, Burbelo PD, Yamada Y, et al. Mast cells chemotax to laminin with enhancement after IgE-mediated activation. J Immunol 1989; 143: 4188-92.
34. Chakravarty N, Yu WJ. Regulatory role of calcium on histamine secretion. Agent Actions 1986; 18: 57-60.
35. Pearce FL, Ennis M, Truneh A, White JR. Role of intra- and extracellular calcium in histamine release from rat peritoneal mast cells. Agent Actions 1981; 11: 51-4.
36. Knudsen T, Johansen T. The mode of inhibition of Na+-K+ pump activity in mast cells by calcium. J Pharmacol 1989;
98: 1119-26.
37. Knudsen T, Johansen T. Na+-K+ pump activity in rat peritoneal mast cells: inhibition by extracellular calcium. Br
J 1989; 96: 773-8.
38. Bertelsen H, Johansen T. Extracellular magnesium decreases the secretory response of rat peritoneal mast cells to
compound 48/80 in vitro. Life Sciences 1991; 49: 1689-97.

Thank you for copying data from http://www.arastirmax.com