Cultivation of Trichomonas intestinalis in CPLM media
Journal Name:
- İnönü Üniversitesi Tıp Fakültesi Dergisi
Key Words:
Keywords (Original Language):
| Author Name | University of Author | Faculty of Author |
|---|---|---|
Abstract (2. Language):
Trichomonas intestinalis is a flagellated protozoon which is located especially in the ileocecal part of the human intestine and it is widespread throughout everywhere in the world. However it is said to cause rarely an abdominal pain, mucoid diarrhoea periods and some deficiencies in the intestine, some searchers declared as it is nonpathogen.
Diagnosis of T. intestinalis can be made by way of observing the motile trophozoids in the stool with direct
microscopic examination. And various kinds of methods are sugggested for cultivation of this protozoon.
in this study, the cultivation of T. intestinalis has been tried at CPLM media used widespreadly in the parasitology
laboratories.
T. intestinalis containing stool samples which were examined under light microscope in the inönü University Medical Faculty Department of Parasitology were cultivated in the CPLM media and reproduction was checked every other day. it was observed that the T. intestinalis can survive for 16 days in CPLM culture. As a result, CPLM medium is available for diagnosis of intestinal Trichomaniasis and trophozoids may be used in educational studies.
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Abstract (Original Language):
Trichomonas
intestinalis, dünyanin her tarafinda insan bagirsaginda özellikle barsagin ileo-çekal kesiminde bulunan bagirsak kamçilisidir. Çok nadiren karinda buruntulu agri, mukuslu diyare periyodlari ve bazi bagirsak bozukluklarina neden oldugu bildirilmisse de arastiricilar patojenligi üzerine görüs birliginde degildirler. T. intestinalis'in tanisi direkt mikroskopik diski bakisinda hareketli trofozoitlerin görülmesiyle konmaktadir. Ayrica bu protozoon için çesitli besiyerleri de tanimlanmistir.
Çalismada, T. intestinalis'in birçok parazitoloji laboratuvarinda yaygin olarak kullanilan cysteine-peptone-liver-maltose (CPLM) besiyerinde üretilmesi denenmistir. inönü Üniversitesi Tip Fakültesi Parazitoloji Anabilim Dali laboratuvarina parazit bakisi için gelen ve direkt bakida T. intestinalis saptanan diskilar CPLM besiyerine ekilmis ve iki günde bir kontrol edilerek üreme olup olmadigi degerlendirilmistir. CPLM besiyerinde T. intestinalis trofozoitlerinin 16 gün süre ile canliliklarinin devam ettigi gözlenmistir.
CPLM besiyerinin T. intestinalis 'in tanisinda kullanilabilecegi ve üretilen parazitlerin egitim amaçli degerlendirilebilecegi sonucuna varilmistir.
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