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Various Culture Media Effect on T4 Phage Lysis and Production

Journal Name:

  • International Journal of Innovation and Applied Studies

Publication Year:

  • 2013

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Abstract (Original Language): 
Studies on bacteriophage growth and its development played a vital role in the history of molecular biology which in turn helped in clarification of many points. Most of the previous studies on bacteriophage development and growth have been performed under optimal conditions for the host cell. While On the other hand these conditions may not be optimal for the T4 bacteriophage. As a matter of fact in nature E. coli faces many unfavorable growth conditions, good example are those conditions prevailing in the human gut in which E.coli manages to survive well. This study characterizes the effects and influences of well-defined physiological conditions on T4 bacteriophage growth and development. In addition to this, T4 bacteriophage interactions with its bacterial host have also been demonstrated. In our present study we observed that the maximum growth and lysis of T4 bacteriophage was on luria-bertani (LB) and nutrient media (NM). Moreover the T4 bacteriophage production and lysis was also good in luria-bertani plus glucose (LB+G) media but when compared with its production in luria-bertani (LB) and nutrient media it was found to be less than these medium. Our study results also showed that in minimal media (MM) rate of growth and lysis activity of T4 bacteriophage was lowest as compared to other mentioned medium.
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REFERENCES

References: 

[1] J.D. Karam, Molecular Biology of Bacteriophage T4, Washington, DC: American Society for Microbiology, 1994.
[2] J. Cairns, G.S. Stent, and J.D. Watson, “Phage and the Origins of Molecular Biology,” Cold Spring Harbor, NY: Cold Spring
Harbor Laboratory, 1966.
[3] E.L. Ellis and M. Delbruck, “The growth of bacteriophage,” J Gen Physiol, vol. 22, no. 3, pp. 365-384, January 1939.
[4] H. Doermann, “The intracellular growth of bacteriophage,” Carnegie Inst Wash Year Book, vol. 47, pp. 176-182, 1948.
[5] E. Goldberg, L. Grinius, and L. Letellier, Recognition, attachment, and injection, In Molecular Biology of Bacteriophage
T4, edited by J. D. Karam. Washington, DC: American Society for Microbiology. pp. 347-356, 1994.
[6] H. Hadas, M. Einav, I. Fishov, and A. Zaritsky, “Bacteriophage T4 development depends on the physiology of its host
Escherichia coli,” Microbiology, vol. 143, pp. 179–185, 1997.
[7] I.N. Wang, D.L. Smith, and R. Young, “HOLINS: the protein clocks of bacteriophage infections,”Annu Rev Microbiol, vol.
54, pp. 799–825, August 2000.
Various Culture Media Effect on T4 Phage Lysis and Production
ISSN : 2028-9324 Vol. 4 No. 3, Nov. 2013 546
[8] L. Sula and J. Sulova, “A comparative study of special agar medium (Redmond) and simple agar medium for the phage
typing of mycobacteria,” Bull Wld Hlth Org, vol. 41, no. 1, pp. 17-19, 1969.
[9] W.H. Price, “Phage formation in staphylococcus muscae cultures”, J Gen Physiol, vol. 35, no. 5, pp. 741–759, May 1952.
[10] J.H. Northrop,” Increase in bacteriophage and gelatinase concentration in cultures of bacillus megatherium,” J Gen
Physiol, vol. 23, no. 1, pp. 59-79, September 1939.
[11] J. Sambrook and D.W. Russell, “Molecular Cloning, a Laboratory Manual,” Cold Spring Harbor Laboratory Press, Cold
Spring Harbor, NY, 2001.
[12] R.G.E. Murray, R.N. Doetsch, and C.F. Robinow, Determinative and cytological light microscopy, In: Methods for General
and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P, PRGE Murray, WA Wood and NR Krieg. Washington, DC.
American Society for Microbiology, 1994.
[13] M.H Adams, Bacteriophage, Inter science Publishers, New York, pp. 450– 456, 1959.
[14] W.R. Redmond, “Ward Media and methods for phage typing Mycobateria,” Bull Wld Hlth Org, vol. 35, pp. 563-568,
1966.
[15] H. Bremer and P.P. Dennis, Modulation of chemical composition and other parameters of the cell by growth rate, In:
Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, edited by F. C. Neidhardt, J. L. Ingraham, K.
Brooks Low, B. Magasanik, M. Schaechter & H. E. Umbarger. Washington, DC: American Society forMicrobiology, pp.
1527-1542, 1987.
[16] J.L. Ingraham, O. Maaløe, and F.C. Neidhardt, “Growth rate as a variable,” In Growth of the Bacterial Cell, Sunderland,
MA: Sinauer Associates, pp. 267-315, 1983.
[17] A.L. Koch, “The adaptive responses of Escherichia coli to a feast and famine existence,” Adv Microb Physiol, vol. 6, 147-
217, 1971.
[18] N.O. Kjeldgaard, O. Maaloe, and M. Schaechter, “The transition between different physiological states during balanced
growth of Salmonella typhimurium,” J Gen Microbiol, vol. 19, pp. 607-616, 1958.

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