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DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND INDAPAMIDE IN THEIR COMBINED DOSAGE FORM

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Abstract (2. Language): 
A simple, precise and accurate high performance thin layer chromatographic method was developed and validated for the simultaneous estimation of amlodipine besylate and indapamide in combined tablet dosage form. Pre-coated silica gel 60F254 aluminium plate was selected as the stationary phase and Ethyl acetate: Toluene: Methanol: Ammonia 5:3.5: 1: 0.5 (v/v/v/v) was used as developing mobile phase. The detection of amlodipine and indapamide was carried out at 240nm. The method was validated for linearity, accuracy, precision, limit of detection and limit of quantitation parameters. The correlation coefficient of amlodipine and indapamide were found to be 0.998 and 0.997 respectively. The average percentage recovery of amlodipine and indapamide were 99.4-100.3 and 99.8¬100.2 respectively. The proposed HPTLC method has potential applications for determination of aml odipine besylate and indapamide in combined tablet dosage form.

REFERENCES

References: 

1. Sharma BK: Instrumental Methods of Chemical Analysis, 20th edition, GOEL Publishing House, 2005:68-80.
2. David H: Modern Analytical Chemistry, De pauw University, 1st edition: 2.
3. http://www.drugbank.ca/drugs/DB00381
4. http://www.drugbank.ca/drugs/DB00808
5.
Dru
g information: Amlodipine besylate and Indapamide: www.Rxlist.com/info/drug
6. Molecular weight of drugs: Amlodipine and Indapamide
7. www.sigmaldrich.com/mol.wt/drug%inf
8.
Mura
t Ozturk and Yucel Kadiogul: Development and Validation of UV
Spectroscopic Method for Determination of Amlodipine Beylate in Spiked Plasma. Fabad J. pharma sci. 2007;1: 1-6.
9. Mehul
P
, Ramesh V, Vinay K, Srinivas R and Diwan P: Simultaneous Spectroscopic Estimation of Amlodipine Besylate and Olmesarten Medoximil in Tablet Dosage Form", Asian Journal and Research Center-2, 2009: 2:13-19.
10.
Josh
i HV and Patel J: New Spectroscopic Method for the Determination of Amlodipine Besylate and Lisinopril in Tablet Dosage Form", Journal of Applied pharmaceutical chemistry 2011; 8: 223-238.
11.
Tallur
i C, Srinivas K, Vijay KB, Ramesh J and Kishore M: Simultaneous Determination Of Amlodipine Besylate And Atorvastatin Calcium In Tablet Dosage Forms By Spectrophotometric Methods", International Journal of Biopharmaceutics
2009; 4: 123-127
12. Vijaya V, Vrushali T and Vrushali D: Spectrophotometric Simultaneous Determination of Amlodipine Besylate and Hydrochlorothiazide in Combined Tablet Dosage Form By Simultaneous Equation, Absorption Ratio And First Order Derivative Spectroscopy Methods.
ISSN: 2277-8713 IJPRBS
International Journal of Chemistry Research 2011; 5: 25-32.
13.
India
n Pharmacopoeia 2007,Government of India ministry of Health & family welfare, published by The Indian Pharmacopoeia Commission, Ghaziabad, Volume 1 and 2, pp
252, 714-5
14.
Ranja
n B, Anwar IM, Maruf A, Mir W, Robiul I and Bytul MR: Simultaneous High-Performance Liquid Chromatographic Determination of Atenolol and Amlodipine in Pharmaceutical-Dosage Form. Pak. J. Pharm.
Sci. 2007: 274-279
15. Mustafa C, Mustafa SK, Sacide A and Selma S: Validated HPLC Method
Development: The Simultaneous Analysis Of Amlodipine And Valsartan In Samples For Liver Perfusion Studies Hacettepe University Journal of the Faculty of Pharmacy 2008;1:
15-30
16. Chitlange SS, Kiran B and Sakarkar DM: stability indicating rp- hplc method for simultaneous estimation of Valsartan and Amlodipine in capsule formulation." Asian J.
Research Chem. 2008;1:69-74
17. Anilkumar S, Bhavesh P and Rakshit P: Simultaneous Estimation Of Nebivolol Hydrochloride And S-amlodipine BesylateBy High Performance Thin Layer Chromatography", International Journal of
Pharma and Bio Sciences, 2010; 4: 339-347
18.
Hann
a MS, Alaa SA and Magda EM: New Spectrophotometric Methods for the Determination of Indapamide and Its
Formulations", 2001: 3; 85-89.
19. Paresh UP and Hetal PP: Two
Spectroscopic Methods for the Simultaneous Estimation of Indapamide and Nebivolol Hydrochloride in Combined Dosage Form", International Journal of Pharmaceutical
Research 2011: 55-57.
20. Patel Amit R and Chandrul Kaushal Kishor: Method Development, Validation And Stability Study For Simultaneous Estimation Of Telmisartan And Indapamide By Reverse Phase-High Performance Liquid
Chromatography In Pure And Marketed Formulation. International Journal on Pharmaceutical and Biomedical Research
2011: 4-16
21. Barot TG, Prajapati V, Patel PK, Shah N, Patel LD: A Validated Rp-HPLC Method
for Simultaneous Estimation of Indapamide Impurity (Methyl Nitrosoindoline) API Form. International Journal of Pharm Tech
Research CODEN (USA), 2008: 56-59
22. Savita SY and Janhavi RR: Simultaneous HPTLC Analysis of Atenolo and Indapamide in Tablet Formulation. Pharmacie Globale International Journal of Comprehensive Pharmacy. 2011; 1: 1-4.
23. ICH Harmonized Tripartite Guidelines, Validation of analytical procedures: Text and Methodology, Q2(R1), Geneva, 2005.

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