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DEVELOPMENT AND VALIDATION OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF AMBROXOL HYDROCHLORIDE AND CEFPODOXIME PROXETILE IN THEIR COMBINED TABLET DOSAGE FORM

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2012

Key Words:

Author Name
Abstract (2. Language): 
The present manuscript describes new, simple, accurate, and precise high performance thin layer chromatography method for the simultaneous determination of Ambroxol Hydrochloride and Cefpodoxime Proxetile in combined tablet dosage form. Chromatographic separation of the drugs was performed on aluminium plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of Chloroform: Methanol: Hexane: Glacial acetic acid (6: 2: 4: 0.2 v/v/v/v). Densitometric evaluation of the separated zones was performed at 245 nm. The two drugs were satisfactorily resolved with Rf values 0.22 and 0.64 for Ambroxol Hydrochloride and Cefpodoxime Proxetile, respectively. The linear regression data for the calibration plots showed good relationship with r2 = 0.99925 from 120-720 ng/spot for Ambroxol Hydrochloride and r2 = 0.99897 from 200-1200 ng/spot for Cefpodoxime Proxetile. The methods were validated for precision, accuracy, and recovery. The percentage recovery for Ambroxol Hydrochloride was found to be 99.75 - 100.19 % and 99.86 - 100.02% for Cefpodoxime Proxetile. The limits of detection and quantification were 21.16 and 64.11 ng/spot per spot for Ambroxol Hydrochloride and 41.16 and 124.72 ng/spot per spot for Cefpodoxime Proxetile, respectively.
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