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UV SPECTROPHOTOMETRIC METHOD FOR IDENTIFICATION AND ESTIMATION OF CLONAZEPAM IN TABLET DOSAGE FORM

Journal Name:

  • The International Journal of Pharmaceutical Research and Bio-Science

Publication Year:

  • 2012

Key Words:

Author NameUniversity of Author
Abstract (2. Language): 
The present research work describes the spectrophotometric method for the identification and estimation of Clonazepam in bulk and pharmaceutical dosage form. Identification test is proposed based on distinctive UV spectra of Clonazepam under acidic and alkaline condition. The drug exhibited UV absorpti on maximum at 271 nm in 0.01 N HCl which underwent Hyper chromic and Blue shift to 263 nm when sample was heated in boiling water bath for 2 hours. The drug exhibited UV absorption maximum at 364 nm in 0.01 N NaOH which underwent Hypo chromic and Red shift to 387 nm when sample was heated in boili ng water bath for 2 hours. For the quantitative estimation, the stock solution was prepared in methanol and the drug was determined at absorption maximum of 309 nm. Beers law was obeyed in the concentration range of 4-28 |ig/ml having regression line equation y = 0.0377x + 0.0005 with correlation coefficient of 0.9998. Results of the analysis were validated statistically and recovery was found between 98.81-1 01.03%. The method can be used for routine quality control analysis of Clonazepam formulation
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REFERENCES

References: 

1.
Parker WA
Epilepsy
. In: Herfindal ET, Gourley DR, Hart LL (Eds) Clinical pharmacy and therapeutics. Williams and Wilkins, Maryland 1988: 585-586
2. Martindale: The Complete Drug Reference, Pharmaceutical press, 36th
Edition 2009: 478-479.
3. The Indian Pharmacopoeia, The Indian Pharmacopoeia commission, Ghaziabad, 6th edition 2010, Vol.-II: 1111-1114.
4. British Pharmacopoeia, British Pharmacopoeia Commission 2007; Vol I: 546. Vol III: 2444-2445.
5.
Unite
d state pharmacopoeia, Rockville, USP convention, Inc, 30th edition 2007: 1795-1797.
6. European Pharmacopoeia monograph: Clonazepam available online at http://www.sinoapi.com/pharmacopoeia/p harmacopoeia-ep6.asp?cas=1622-61-3
(Accessed on 24/07/2011)
7.
Japanes
e Pharmacopoeia monograph: Clonazepam available online at http://www.sinoapi.com/pharmacopoeia/p harmacopoeia-jp15. asp?cas=1622-61 -3
(Accessed on 24/07/2011)
ISSN: 2277-8713 IJPRBS
8. Christopher J and James TS: Analysis of Clonazepam in a tablet dosage form using small bore HPLC, Journal of Pharmaceutical and Biomedical Analysis 1998, 18: 453-460.
9.Isabelle Forfar Bares, Fabienne Pehourcq and Christian Jarry: Development of a rapid RP-HPLC method for the determination of clonazepam in human plasma, Journal of Pharmaceutical and Biomedical Analysis 2004, 36: 865-869.
10. Mistsuhrio Nakamura, Kana Fukava, Tadashi Sugiyama and Yoshihiro Katagiri: High-Performance Liquid Chromatographic Assay of Clonazepam in Human Plasma Using a Non-porous Silica Column, Biol. Pharm. Bull 2004; 27: 893¬895.
11. BH Min and WA Garland: Determination of Clonazepam and its 7-Amino Metabolite in Plasma and Blood by Gas Chromatography- Chemical Ionization Mass Spectrometry, Journal of
Chromatography 1977; 139:121-133.
12. Wang Zengshou, Zhu Guanghui and Li Guangqian: Determination of Phenobarbital, phenytoin, Carbamazepine and Clonazepam in serum by HPLC, TheChinese Journal of Modern Applied Pharmacy 2002; 2: 155-156.
13. J. Arthur FS, Carl
VP
, and Nancy M: Determination of Clonazepam and flunitrazepam in blood and urine by electron-capture GLC, Journal of Pharmaceutical Sciences1974; 63: 520¬527.
14.
Internationa
l Conference on Harmonization ICH harmonized tripartite guideline, Validation of analytical procedures: text and methodology Q2 (R1). ICH, Geneva 2005.

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