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PREVENTION OF RADIATION - INDUCED BIOCHEMICAL CHANGES IN MOUSE BRAIN BY PUNICA GRANATUM

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Abstract (2. Language): 
Ionizing radiations cause damage to living tissues and biomolecules. Radiation exposure damages the nervous system also. In the present study Punica granatum fruit rind extract (PGFRE) was tested to protect radiation induced changes in brain biochemistry. For experimental purpose, healthy adult male Swiss albino mouse (25 ±2g) were selected and divided into four groups. Group-I was kept without any treatment (normal). Group-II was irradiated with 8Gy Co60 gamma radiation only (control). Group-III was fed PGFRE (acetone) (10 mg/kg body weight) one hour before irradiation (experimental) and Group IV was given with PGFRE only. Mice were sacrificed at different intervals and brain was removed, weighed and analysed for estimation of its glutathione (GSH), lipid peroxidation (LPO), protein, DNA and RNA content. Brain weight of the control and experimental groups decreased till 3rd day post irradiation but remained higher than the control. Protein and RNA contents increased up till the 3rd post irradiation day and decreased till 28th day in control but were at higher level in the experimental group. GSH and DNA content decreased in control and experimental group but found to be higher than the corresponding control group at all the intervals. Thus, results suggest that pretreatment of PGFRE protects mouse brain against the radiation induced biochemical changes.

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