Journal Name:
- The International Journal of Pharmaceutical Research and Bio-Science
Key Words:
Author Name |
---|
Abstract (2. Language):
The study was conducted to determine the phytoconstituents and to determine antibacterial
potential including the minimum inhibitory concentration (MIC) of the leaf extracts of Withania somnifera and
Eucalyptus melliodora against pathogenic strains of bacteria viz. Nocardia asteroids and Streptococcus pyogenes.
Aqueous and methanolic plant extracts were screened for their antibacterial activity by in vitro agar well diffusion
and disc diffusion methods against the microbial strains collected from MTCC, Chandigarh. MIC was determined
by dilution series test. The zones of inhibition against N. asteroids by methanolic extracts of E. melliodora and W.
somnifera by agar well diffusion were 16 and 20 mm whereas in case of disc diffusion method they were 10 and
15 mm respectively. Zones of inhibition noted against S. pyogenes were 22 mm and 23 mm via agar well diffusion
method and 11 mm and 13 mm by disc diffusion method with extracts of E. melliodora and W. somnifera
respectively. The aqueous extracts were not showed any reliable results against both the pathogens using disc
diffusion method. Minimum Inhibitory Concentration (MIC) obtained were 31μg/mL and 62μg/mL against both
the pathogens viz. N asteroids and S. pyogenes with E. melliodora and W. somnifera respectively. The
phytochemicals viz. alkaloids, flavanoids, saponins, tannins, carbohydrates and proteins were found to be present
in extracts of both the plants.
Bookmark/Search this post with
- 5