Isolating Antifidan Compounds of Kepyar Castor Seeds (Ricinus Communis L) to the Beetle Epilachna Varivestis Mulsant, (Coleoptera: Coccinelidae)

As much as 2 kg kepyar castor beans (Ricinus communis L) that has been granulated, then it was macerated for 3 x 24 hours using 3.0 L of methanol solvent got crude extract obtained 387.2 grams. Methanol crude extract was partitioned and fractionated with ethyl acetate and n-hexane to obtain ethyl acetate fraction, the fraction of n-hexane and methanol extracts. Three fractions were tested antifidan activity against larvae Epilachna varivestis. Antifidan test results showed that the methanol extract gave the highest antifidan activity (65%) followed etyl acetate fraction (59%) and n-hexane fraction (58%). Methanol extracts were isolated and purified by chromatography column techniques and thin layer chromatography. The first column got three fractions as Fraction B1 = 1.23 grams, as Fraction B2 = 12.34 grams and a fraction B3as much = 1.42 grams). The results of antifidan activity test of three fractions obtained B2 fraction showed the highest inhibitory activity ( Fr. B2 = 70%), (Fr. B1 = 53%) and Fr. B3 = 52.72. The three fractions was TLC tested and B2 fractions showed crystals with two stains, so it must be columned again. In the second column used eluen n-hexane: ethyl acetate fraction obtained two B2.1 (1.31 grams) and Fraction B2.2 (6.019 grams). Fraction B2.2 was tested by TLC showed a single stain then tested the two-dimensional TLC showed a single stain. Fraction B2.2 antifidan activity assays provide meals inhibition (antifidan) higher (B2.2 79%) whereas only give a fraction B2.1 inhibition of eating (B2.1 = 58%). Analysis and identification of the IR and NMR note that there are two compounds of the seed that have the same core but different in substituen and compound classes of alkaloids and terpenoids indicated