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SAĞLIKLI İNSANLARDA ERİTROSİT MEMBRANI Na+-K+ATPaz VE Ca2+ ATPaz AKTİVİTELERİNE VE SİALİK ASİD DÜZEYLERİNE YAŞLANMANIN ETKİSİ

Effect of aging on Erythrocyte Membane Na+-K+ATPase and Ca2+ ATPase Activities and Sialic Acid Levels in Healthy Subjects

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 1999

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
This study was planned to investigate the changes on erythrocyte membrane Na+-K+ATPase ve Ca3+ ATPase activities and sialic acid levels that may be related to aging. An insignificant decrease was obtained in the crythracyte membrane Na+-K+ATPase and Ca2+ ATPase activities of 14 old subjects wİth a mean age of 64 accor-ding to the enzyme activities of the 14 young subjects whose mean age was 28. The İntracellu-lar and extracellular Na+ and K+ concentrations and the plasma and erythrocyte membrane sİ-alİc levels were found with a tendeney to inerease in the elder subjects but only the plasma Na+ inerease was significant. According to these findings, it is thougt these two enzyme activities were not affected with inereasing age and (he aging process do not make some negative changes on celi volume and homeostasis mechanisms and on membrane stmeture.
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Abstract (Original Language): 
Bu çalışma, eritrosit membram Na+-K+ATPaz ve Ca2+ ATPaz aktiviteleri ile sialik asid düzeyleri üzerine yaşa bağımlı olabilecek değişiklikleri araştırmak için planlanmıştır. Yaş ortalaması 64 olan 14 yaşlı olgunun eritrosit membram Na+-K+ATPaz ve Ca2+ ATPaz aktivitelerin-de, yaş ortalaması 28 olan 14 gencin enzim aktivitelerine göre anlamlı olmayan azalma saptandı. Hücre içi ve dışı Na+ ve K+ konsantrasyonları ile plazma ve eritrosit membram sialik asid düzeyleri yaşlılarda artmaya eğilimli bulundu, ancak yalnız plazma Na+ artışı anlamlı idi. Bu bulgulara göre; her iki enzim aktivitelerinin ilerleyen yaş ile etkilenmediği ve yaşlanma olayının hücre hacmi ve homeostazisi mekanizmalarında ve membran yapısında organizma aleyhine olabilecek değişikliklere yol açmadığı düşünüldü.
FULL TEXT (PDF): 
PDF icon arastirmax-saglikli-insanlarda-eritrosit-membrani-na-katpaz-ca2-atpaz-aktivitelerine-sialik-asid-duzeylerine-yaslanmanin-etkisi.pdf
  • 3
307-310
  • Turkish

REFERENCES

References: 

1. Allalouf D, Komlos L, Nolman J, Halbrecht I, Levinsky H: Sialic acid content and sialyitransferase acüvity in humarı lymphocytes with advancing age. Mech Ageing Dev 44:45 (1988).
2. Amano M, hnata K, Suzuki K, Nakaoka H, Fuji J: Age-TCİaiedreduction in the nıımber of rabbit erythrocyte Na, K-ATPase. Tohoku J Exp Med 159:131 (1989)
3. Bozzo C, Goria M, Marena S, Velgia F, Pagano G: Lymphocyte Na-K-ATPase is rcduced in aged people. Mctab. 8:808 (1990).
4. Cumberbatch M, Morgan DB: Relations between sodium transport and sodium eoncentration in human erythrocy-tes in health and disease. Cliıı Sci. 60:555 (1981).
5. Davis PJ, Blas SD: İn vİtro stimulation of human red blo-od celi Ca2+ ATPase by thyroid hormone. Biochem Bi-ophys Res Commun 99:1073 (1981).
6. Denyy PC, Dcnny PA, Allerton SE: Determînatİon of sialic acid using 2-thiobarbituric acid in the absence of ha-zardous sodium arsenile. Clin Chİm Açta 131:333 (1983).
7. Est M, Erjavec E, Krzan M: Age-related clıanges chan-
ges of eıythrocytc Na+-K+ATPase activity. lugoslav Physiol Pharmacol Açta 25:35 (1989).
8. Hcnnesy J F: Vascular tissue Na+-K+ATPase activity and aging in the P344 rat. Mech Ageing Dev 43:153 (1988).
9. Lowry OH, Rosebrough NJ, Farr A.L, Randall R.J.: Protein measurement vvith the Folinphenol reagent. J Biol Chem 193:265(1951).
10. Naylor GJ, Dick DAT, Worall EP, Dick EG, Dick P, Bo-ardman L: Clıanges in the erytrocytc sodium pump wilh age. Gcrontology 23:256 (İ977).
11. Paci A, Cocci F, Cristofani R, Balzan S, Mezzasalma L, Piras F, Giachetti M, Ghione S: Comparison of inlra erythrocyte and intralcucocyte sodium content and erythrocyte fragility in normolensive subjects. J Nuclear Med Allicd Sd 32:101 (1988).
12. Rahman M, Koh H, Primera MI, Del Grmcco F, Quinta-nilla AP: Na-K-Adenosinc triphosphatasc and cation content in the erythrocyte in essentia! hypertension. J LabClin Med İ07:337 (1986).
13. Samaja M, Rubinacci A, Motterlini R, De Ponti A, Posti-naro N: Red celi ageing and aetive caleium transporl. Exp Gerontol 25:279 (1990).
14. Schmalzing G, Pfaff E, Brcyer-Pfaff U: Red celi oııbain binding sites, Na+-K+ATPasc and intracellular Na+ as individual characleristics. Life Sci 29:371 (1981).
15. Schulte BA, Sclımiedt RA: Lateral Wall Na+-K+ATPase and endocochlear potcnlials decline vvilb age in quietrca-ted gcrbils. Hearing Res 61:35 (1992).
16. Simat BM, Morley JE, From AHL, et al.: Variables af¬feeting measurement- of-human-red eell-Na+-K+ATPase
activity tcchnical factors fecding, aging. Am J Clin Nutr 40:339 (1984).
17. Stefenelli N, Klotz H, Engel A, Bauer P: Serum sialic asid in malignanl lumors, bacterial infeciions and chıonic liver diseases. J Cancer Res Clin Oncol 109:55 (1985).
18. Stiblcr H, Sydow O: The siaüc acid and galactose con-centrations in erythrocyte membranes in palients with mytonic dystrophy, limp-girdlc and facioscapulo hume-rai dystrophy. J Nctırologicai Sci 59:389 (1983).
19. Sydow G: A simplifıed quick metod for delerminatiort ot sialic acid in serum. Biomed Biochim Açta 44:1721 (1985).
20. Taussky HH, Shorr E: A miero colorimetric method for determination of inorganic phosphorus. J Biol Chem 202:675 (1953).
21. Tozzi-Ciancarclli MG, Fedeîe F, Tozzi E, et al: Age-de-pendent clıanges in human erythrocyle properties. Clin Ilemorheology 9:999 (1989).
22. Wood L, BeulJer E: Tempcralure dependence of sodium potassiunı aelivated erythrocyte adenosine triphosphata-se. J Lab Clin Med 70:288 (1967).

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