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POLİKİSTİK BÖBREK HASTALIĞINDA APOPTOZ

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2001

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Apoptosis in polycystic kidney disease. Increased apoptosis is reported in different models of polycystic kidney disease. However, the role of apoptosis in cystogenesis and in fhe develop-ment of renal failure is not known. The Han:Sprague-Dawley (SPRD) rat model phenotypi-cally resembles human autosomal dominant polycystic kidney disease. Therefore, it presents a model to investigate the pathogenesis of this disease. Han:SPRD rats were studied during the early stages (1 day old and 2 weeks old) of their disease. Homozygous (Cy/Cy) rats had massive cystic development and renal failure at 2 weeks of age. Apoptotic cells were detected in kidney tissues by the TÜNEL (Tdt-mediated dUTP nick end-labeling) assay. TÜNEL (+) cells were significantly increased in 2-week-old heterozygous (Cy/+) and Cy/Cy rat kidneys compared to normal (+/+) littermate controls. TÜNEL (+) cells were observed both in cystic and non-cystic tubules. In conclusion, increased apoptosis may play a role in the development of renal failure in polycystic kidney disease.
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Abstract (Original Language): 
Apoptoz artışı, çeşitli polikistik böbrek hastalığı modellerinde bildirilmiştir. Buna karşılık, apoptozun kist patogenezinde ve böbrek yetersizliği gelişimindeki rolü bilinmemektedir. Han:Sprague-Dawley (SPRD) sıçan modeli, insandaki otozomal dominant polikistik böbrek hastalığına fenotipik benzerlikler gösterir. Bu nedenle, bu hastalığın patogenezinin araştırılması için iyi bir modeldir. Han:SPRD sıçanları, hastalığın erken döneminde (1. gün ve 2. hafta) çalışmaya alındı. Homo-zigot (Cy/Cy) olan sıçanlarda 2. haftada yaygın kist oluşumu ile birlikte böbrek yetersizliği gelişimi gözlendi. Böbrek dokularındaki apoptotik hücreler, TÜNEL (Tdt-mediated dUTP nick end-labeling) yöntemi ile araştırıldı. İki haftalık heterozigot (Cy/+) ve Cy/Cy sıçanların böbrek dokularında, normal (+/+) sıçanlara göre TÜNEL (+) hücrelerde anlamlı derecede artma saptandı. TÜNEL (+) hücrelerin hem kistik, hem de kistik olmayan tubuluslarda görüldüğü dikkati çekti. Sonuç olarak, polikistik böbrek hastalığındaki apoptoz artışının böbrek yetersizliğinin gelişiminde rolü olabilir.
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  • 4
168-173
  • Turkish

REFERENCES

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