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DNA, PROTEİNLER VE LİPİTLERDE OKSİDATİF DEĞİŞİMLER ÜZERİNE YAŞLANMANIN ETKİSİ: LENFOSİTLER, ERİTROSİTLER, PLAZMA VE DÜŞÜK DANSİTELİ LİPOPROTEİNLERDE İNCELEMELER

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2003

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Abstract (2. Language): 
The effect ofaging on oxidative modificalions in DNA, proteins and lipids: The investigations in lymphocytes, erythrocytes, plasma and low âensity iipoproteins. Free radicals play an im-portant role in the proccss of aging. In this study, we wanted to investigale the rclatioııship between the oxidative stress and aging in humans by determhıing lipİd and protein oxidatİon in plasma as well as DNA damage in lymphocytes of 55 healthy subjects who werc divided into two subgroups of age; young (21-40 years) and clderly (61-85 years). In addition, we dc-termined endogenous and stimulated Üpid peroxide levels in low density İipoproteins (LDL) and erythrocytes of young and eiderly subjects. According to our resuits, Üpid peroxide and protein carbonyl levels in plasma as well as DNA damage in lymphocytes is found to inerease in clderly subjects, lipİd peroxide levels also inereased in LDL and erythrocytes of eiderly subjects. Our resuits, clearly show the presence of oxîdative stress in eiderly subjects.
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Abstract (Original Language): 
Serbest radikaller yaşlanmada önemli rol oynamaktadır. Çalışmamızda, genç (21- 40 yaş) vc yaşlı (61-85 yaş) olarak iki alt gruba ayrıları 55 sağlıklı kişinin plazmalarında lipit ve protein oksidasyonu, lenfositlerinde DNA hasarı tayin edilerek oksidatif stres ile yaşlanma arasındaki ilişki araştırılmak istendi. Ayrıca, genç ve yaşlı kişilerin düşük dansiteli Iipoproteinlerİnde (LDL) ve eritrositlerinde endojen ve uyarmalı lipit peroksit düzeyleri Ölçüldü. Sonuçlarımıza göre, yaşlı kişilerin plazmasında lipit peroksit ve protein karbonil düzeylerinin, lenfositlerden elde edilen DNA'da oksidafif hasarın arttığı bulundu. Ayrıca, yaşlı kişilerde LDL ve eritrositlerde lipit peroksit düzeylerinin arttığı saptandı. Bu sonuçlara göre, yaşlı kişilerde oksidatif stresin arttığı açıkça görülmektedir.
FULL TEXT (PDF): 
PDF icon arastirmax-dna-proteinler-lipitlerde-oksidatif-degisimler-uzerine-yaslanmanin-etkisi-lenfositler-eritrositler-plazma-dusuk-dansiteli-lipoproteinlerde-incelemeler.pdf
  • 2
85-88
  • Turkish

REFERENCES

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