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C6 SIÇAN GLİOMA HÜCRELERİ ÜZERİNE ALOE EMODİN VE CİSPLATININ ETKİLERİNİN İKİ VE ÜÇ BOYUTLU HÜCRE KÜLTÜR MODELLERİNDE İNCELENMESİ

THE EFFECTS OF ALOE EMODIN AND CISPLATIN ON RAT GLIOMA CELL LINE IN TWO AND THREE-DIMENSIONAL CELL CULTURE MODELS

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2006

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: The aim of this study was to examine the effects of aloe emodin, a natural anthraqinone derivate, and cisplatin, a chemotherapeutic drug from organoplatins group, on C6 glioma cell line with the use of two and threedimensional tumour cell culture models. Materials and methods: For this purpose, the drugs were administered both alone and in combination for 12, 24, 48, 72 hours and the effects on cell proliferation and the S-phase fraction were determined in two-dimensional cell culture. In three-dimensional cell culture, the effects of two drugs used alone and in combination were studied at 72 h by determining the S-phase fraction and evaluating the ultrastructure of the cells. Results: Aloe emodin has reduced cell proliferation and S-phase fraction in two-dimensional cell culture (p<0.0125). Aloe emodin has also reduced S-phase labeling in three-dimensional cell culture (p<0.0125). No significant disruption has been detected in spheroid morphology. Cisplatin has reduced cell proliferation and S-phase fraction in two-dimensional cell culture to a greater extent when compared to aloe emodin. Cisplatin also decreased S-phase labeling index in three-dimensional cell culture significantly when compared to other groups. The overall morphology of the spheroids was observed to be more strongly destroyed compared to aloe emodin and control groups. The findings obtained by administrating the combination of both drugs in two and three-dimensional cultures were similar to those of the cisplatin group.Conclusion: In conclusion, the combination of aloe emodin and cisplatin decreases cell proliferation and S-phase fraction and causes disruption of the morphology of C6 glioma cells in two- and three-dimensional cell culture models, but no aggravation or alleviation of the effects of cisplatin can be observed by aloe emodin treatment.
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Abstract (Original Language): 
Amaç: Bu çal›flman›n amac›, do¤al bir antrakinon türevi olan aloe emodin ve organoplatinler grubundan kemoteropatik bir ilaç olan cisplatinin ayr› ayr› ve birarada kullan›ld›klar›nda C6 glioma hücre soyu üzerine etkilerini iki ve üç boyutlu tümör hücre kültür modelleri kullan›larak incelemekti. Gereç ve yöntem: ‹ki boyutlu kültürlerde iki ilac›n ayr› ayr› ve birlikte kullan›mlar› 12, 24, 48, 72. saatlerde hem hücre ço¤almas›, hem de hücre siklusu sentez faz› aç›s›ndan incelendi. Üç boyutlu hücre kültürü modelinde ise her iki ilac›n ayr› ayr› ve kombine kullan›mlar› 72. saatte hücre siklusu sentez faz› ve hücre ince yap›s› aç›s›ndan de- ¤erlendirildi. Bulgular: Aloe emodin, iki boyutlu kültürde hücre ço¤almas›n› azalt›c› etki gösterdi ve hücre siklusu sentez faz› iflaretlenmesinde azalma meydana getirdi (p<0,0125). Üç boyutlu kültürde de sentez faz›nda azalmaya yol açt›¤› görüldü (p<0,0125). Sferoid morfolojisinde belirgin bir bozukluk tespit edilmedi. Cisplatin de iki boyutlu kültürde hücre ço¤almas›n› azaltt› ve hücre siklusu sentez faz› iflaretlenmesinde daha belirgin azalma meydana getirdi (p<0,0125). Üç boyutlu kültürde de sentez faz›nda daha etkin azalmaya yol açt›¤› görüldü (p<0,0125). Sferoidlerin genel morfolojileri kontrol ve aloe emodin grubuna göre bozuktu. Kombinasyon grubunda iki ve üç boyutlu kültürlerde elde edilen bulgular cisplatin grubunda elde edilen bulgularla benzerdi. Sonuç: Aloe emodin ve cisplatin kombine uyguland›klar›nda C6 glioma hücreleri üzerine etkileri iki ve üç boyutlu kültür modellerinde incelendi¤inde hücre siklusunda sentez faz›ndaki hücrelerin say›s›nda azalmaya yol açarak hücre proliferasyonunu azaltt›¤› ve hücre yap›s›nda bozulmaya yol açt›¤› gözlendi. Ancak aloe emodinin cisplatinin etkisini artt›r›c› ya da azalt›c› yönde bir etkisinin bulunmad›¤› da tespit edildi.
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  • 4
110-116
  • Turkish

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