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AKNE VULGARİSTE ENDOTELİN-1/NİTRİK OKSİT DENGESİ

THE EQUILIBRIUM BETWEEN ENDOTHELIN-1/ NITRIC OXIDE IN ACNE VULGARIS

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: To investigate plasma ET-1 and NOx (nitrate/ nitrite- two end products of nitric oxide metabolism) in acne vulgaris and to evaluate if there is any relathionship between these parameters and disease severity. Materials and methods: Plasma samples of 30 patients with acne vulgaris and 20 healthy controls were investigated by means ET-1 and NOx values. Results: Plasma ET-1 and NOx in patients with acne vulgaris were significantly increased (p<0.01) in comparison with controls. There was a weak, but significant negative correlation between ET-1 and NOx in control subjects (r= -0.456, p< 0.05). There was no correlation between ET-1 and NOx in the study group (r= -0.195, p= 0.302). Among the study group there was a significant correlation between ET-1, NOx and duration of disease (r= 0.412, p< 0.05 and r= 0.570, p< 0.01, respectively). There was no correlation between degree of acne lesions and ET-1 or NOx levels in study group. Conclusion: Increased plasma ET-1 and NOx concentrations in acne vulgaris are probably result of and/or reason for the accentuated ductal hypercornification, hyperkeratinization and sebum accumulation. The increased production of ET-1 and NO by keratinocytes may function as growth and cytotoxic factors and potential mitogens. The lack of correlation between ET-1 and NOx in patients with acne vulgaris suggests that the ET-1/NOx equilibrium may be disturbed, leading subsequently to formation of acne lesions
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Abstract (Original Language): 
Amaç: Akne vulgaris’te plazma ET-1 ve NOx (nitrik oksidin metabolitleri olan nitrat/nitrit) konsantrasyon düzeylerini tayin etmek, ayr›ca bu iki parametre ve hastal›¤›n derecesi aras›nda bir iliflki olup olmad›¤›n› incelemek. Gereç ve yöntem: Akne vulgaris tan›s› konmufl 30 hasta ile 20 sa¤l›kl› kiflinin plazma örneklerinde ET-1 ve NOx ölçümlendi. Bulgular: Akne vulgarisli hastalarda kontrol grubuna göre plazma ET-1 ve NOx düzeyleri anlaml› (p< 0,01) bir art› fl gösterdi. Kontrol grubunda ET-1 ve NOx aras›nda zay›f, fakat anlaml› bir negatif korelasyon (r= -0,456, p< 0,05) bulundu. Çal›flma grubunda ise ET-1 ve NOx düzeyleri aras›nda bir korelasyon bulunmad› (r= -0,195, p= 0,302). Ayr›ca, çal›flma grubunda ET-1, NOx düzeyleri ve hastal›k süresi aras›nda anlaml› korelasyonlar (r= 0,412, p< 0,05 ve r= 0,570, p< 0,01) oldu¤u gözlendi. Akne lezyonlar›n›n derecesi ve ET-1, NOx düzeyleri aras›nda bir korelasyon bulunmad›. Sonuç: Akne vulgariste görülen plazma ET-1 ve NOx düzeylerindeki yükselifl muhtemelen artm›fl duktal hiperkornifikasyon, hiperkeratinizasyon ve sebum birikiminin sebebi ve/veya sonucu olabilir. Artm›fl olan ET-1 ve NOx üretimi keratinositlerde büyük olas›l›kla büyüme, sitotoksik ve mitojenik factor gibi davran›yor olabilir. Çal›flma grubunda ET-1 ve NOx aras›nda korelasyonun bulunmamas›, akne lezyonlar›n›n oluflmas›na yol açan bu iki parametre aras›ndaki dengenin bozuldu¤unu yans›tmaktad›r.
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  • 4
101-105
  • English

REFERENCES

References: 

1. Anggard E. Nitric oxide: mediator, murderer and medicine.
Lancet 1994; 343: 1199-1206.
2. Basal E, Jain A, Kaushal GP. Antibody response to crude cell
lysate of propionibacterium acnes and induction of pro-inflammatory
cytokines in patients with acne and normal healthy
subjects. J Microbiol 2004; 42: 117-25.
3. Bialecka A, Mak M, Biedron R, Bobek M, Kasprowicz A,
Marcinkiewicz J. Different pro-inflammatory and immunogenic
potentials of Propionibacterium acnes and Stapylococcus
epidermidis: Implications for chronic inflammatory acne.
Arch Immunol Ther Exp 2005; 53: 79-85.
4. Bonifati C, Mussi A, Carducci M, Pittarello A, D’Auria L,
Venuti A, Bagnato A, Salani D, Fazio M, Ameglio F. Endothelin-
1 levels are increased in sera and lesional skin extracts
of psoriatic patients and correlate with disease severity. Acta
Derm Venereol (Stockh) 1998; 78: 22-26.
5. Bruch-Gerharz D, Ruzicka T, Kolb- Bachofen V. Nitric oxide
and its implications in skin homeostasis and disease. Arch
Dermatol Res 1998; 290: 643-651.
6. Bull HA, Hottershall J, Chowdhurry N, Cohen J, Dowd PM.
Neuropeptides induce release of nitric oxide from human
dermal microvascular endothelial cells. J Invest Dermatol
1996;106: 655-660.
7. Bull HA, Terenghi G, Bunker CB, Properzi G, Facer P, Polak
JM, Dowd PM. Receptor binding by human cutaneus
microvascular endothelium. J Invest Dermatol 1991;96:
999-103.
8. Gandhi CR, Berkowitz DE, Watkins D. Endothelins: Biochemistry
and pathophysiologic actions. Anestesiology 1994;
80: 892- 905.
9. Graham GM, Farrar MD, Cruse- Sawyer JE, Holland KT,
Ingham E. Proinflammatory cytokine production by human
keratinocytes stimulated with Propionibacterium acnes and
P. Acnes GroEL. Br J Dermatol 2004; 150: 421-428.
10. Grisham MB, Johnson GG, Lancaster JR. Quantitation of
nitrate and nitrite in extracelluler fluids. Methods Enzymol
1996; 268: 237-246.
11. Ignarro, J.L. Signal transduction mechanisms involving nitric
oxide. Biochem Pharmacol 1991; 15; 41: 485-90.
12. Imokawa G, Yada Y, Kimura M, Morisaki N. Granulocyte/
macrophage colony stimulating factor is an intrinsic keratinocyte-
derived growth factor for human melanocytes in
UVA- induced melanosis. Biochem J 1996; 313: 625-631.
13. Imokawa G, Yada Y, Kimura M. Signaling mechanism of endothelin-
induced mitogenesis and melanogenesisin human
melanocytes. Biochem J 1996; 314: 305-312.
14. Imokawa G, Yada Y, Miyagishi M. Endothelins secreted
from human keratinocytes are intrinsic mitogens for human
melanocytes. J Biol Chem 1992; 267: 24675-24680.
15. Jeremy AH, Holland DB, Roberts SG, Thomson KF, Cunliffe
WJ: ‹nflammatory events are involved in acne lesion initiation.
J Invest Dermatol 2003; 121:20-27.
16. Holland DB, Jeremy AH: The role of inflammation in the
pathogenesis of acne and acne scarring. Semin Cutan Med
Surg 2005; 24: 79-83.
17. Kim J, Ochoa MT, Krutzik SR, Takeuchi O, Uematsu S, Legaspi
AJ, Brightbill HD, Holland D, Cunliffe WJ, Akira S,
Sieling PA, Godowski PJ, Modlin RL. Activation of toll-like
receptor 2 in Acne Triggers inflammatory cytokine responses.
J Immunol 2002; 169: 1535-1541.
18. Mills OH, Kligman AM. Ultraviolet phototheraphy and photochemoteraphy
of acne vulgaris. Arch Dermatol 1978; 114:
221-223.
19. Morhenn VB. Langerhans cells may trigger the psoriatic disease
process via production of nitric oxide. Immunol Today
1997; 18: 433-436.
20. Notas G, Xidakis C, Valatas V, Kouroumalis A, Kouroumalis
E. Levels of circulating endothelin-1 and nitrates/nitrites
in patients with virus-related hepatocellular carcinoma. J Viral
Hepatitis 2001; 8: 63-69.
21. Orem A, Aliyazicioglu R, Kiran E, Vanizor B, Cimnocodeit
G, Deger O. The relationship between nitric oxide production
and activity of the disease in patients with psoriasis. Arch
Dermatol 1997;133:1606-1607.
22. Qureshi AA, Lerner LH, Lerner EA. From bedside to the
benchand back. Nitric oxide and the cutis. Arch Dermatol
1996; 132:889-893.
23. Schaller M, Loewenstein M, Borelli C, Jacob K, Vogeser M,
Endothelin-1 and Nitric Oxide in Acne Vulgaris
‹stanbul T›p Fakültesi Dergisi Cilt / Volume: 71 • Say› / Number: 4 • Y›l/Year: 2008
- 104 -
Burgdorf WH, Plewig G. Induction of a chemoattractive proinflammatory
cytokine response after stimulation of keratinocytes
with Propionibacterium acnes and coproporphyrin
III. Br J Dermatol 2005; 153: 66-71.
24. Sirsjö A, Karlsson M, Gidlöf A, Rollman O, Torma H. Increased
expression of inducible nitric oxide synthase in psoriatic
skin and cytokine-stimulated cultured keratinocytes. Br
J Dermatol 1996; 134: 643-648.
25. Suh DH, Kwon TE, Youn JI. Changes of comedonal cytokines
and sebum secretion after UV irradiation in acne patients.
Eur J Dermatol 2002; 12: 139-144.
26. Takuwa N, Takuwa Y, Yanagisawa M, Yamashita K, Masaki
T. A novel vasoactive peptide endothelin stimulates mitogenesis
through lipid turnover in Swiss 3T3 fibroblasts. J Biol
Chem 1989; 264: 7856- 7861.
27. Tsuboi R, Sato C, Shi C. Endothelin-1 acts as an autocrine
growht factor for normal keratinocytes. J Cell Physiol 1994;
159: 213-220.
28. Ural AU, Yalcin A, Beyan C, Isimer A, Bayhan H. Plasma
Endothelin –1 concentrations in patients with Behçet’s disease.
Scand J Rheumatol 1994; 23: 322-325.
29. Vural P, Erzengin D, Canbaz M, Selcuki D. Nitric oxide and
endothelin –1,2 in actinic keratosis and basal cell carcinoma:
changes in nitric oxide/ endothelin ratio. Int J Dermatol
2001; 40: 704-708.
30. Wang R, Chahary A, Shen YJ, Scott PG, Tregdet EE. Human
dermal fibroblasts produce nitric oxide synthase isoforms. J
Invest Dermatol 1996; 106: 419-427.
31. Weller R. Nitric oxide- a newly discovered chemical transmitter
in human skin. Br J Dermatol 1997; 137: 665-672.
32. Yada Y, Higuchi K, Imokawa G. Effects of endothelins in
signal transduction and proliferation in human melanocytes.
J Biol Chem 1991; 266:18352- 18357.
33. Zamora M, Morelli JG, Norris DA, Yohn JJ. Cultured human
keratinocytes syntheise and secrete endothelin-1. J Invest
Dermatol 1992; 98: 646-56.

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