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İNTRAPERİTONEALMELATONİN ENJEKSİYONU VE PİNEALEKTOMİNİN DENEYSEL AKUT PANKREATİTE ETKİSİ

THE EFFECTS OF INTRAPERITONEAL MELATONIN INJECTION AND PINEALECTOMY IN EXPERIMENTALACUTE PANCREATITIS

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2009

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objectives: In this study the aim was to evaluate the effects of on biochemical markers and histopathological findings of pancreas tissue of decreeasing and increasing serum melatonin levels in rats with had experimental acute pancreatitis. Materials and methods: This study was conducted three groups of Winstar Albino rats (ten animals in every group) that have experimental acute pancreatitis. First group was evaluated as control group. Surgical pinealectomy was applied in the second group and the source of endogen melatonin was (low melatonin levels group). Third group of rats injected with 0.1 ml daily intraperitoneal injection of 20 mg/ml melatonin solution for one week was high melatonin levels group. The effects of melatonin levels on acute pancreatitis were evaluated by comparing these group according to histopathologial results of polimorphonuclear leukocytes. infiltration, oedema, haemorrhagie, apoptosis, aciner cellular degeneration in pancreatic tissue and the levels of malonyledialdeyde (MDA), glutathione reductase (GSH), superoxyde dismutase (SOD), aspartate transaminase (AST), alanine transpherase (ALT), amylase, lactate dehydrogenase (LDH) and bilirubine. Results:We investigated that high melatonin levels has an decreasing effect on of polimorphonuclear leukocyte infiltration, oedema, haemorrhage, aciner cellular degeneration, apoptosis in pancreatic tissue, AST, LDH, bilirubine and MDA levels. Conclusion: As a conclusion exogenous melatonin may have a preventive effect on inflammatory process by reducing oxidative damage in pancreatic tissue.
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Abstract (Original Language): 
Amaç: Bu çal›flmada deneysel olarak akut pankreatit oluflturulan ratlarda, kandaki melatonin düzeyinin azalt›lmas› veya artt›r›lmas›n›n pankreas dokusunda neden oldu¤u histopatolojik de¤ifliklikler ve baz› biyokimyasal belirteçlere etkisinin de¤erlendirilmesini amaçlad›k. Gereç ve yöntem: Bu çal›flma deneysel akut pankreatit oluflturulan ve her grupta 10 hayvan bulunan 3 grup Winstar Albino cinsi s›çan üzerinde yürütüldü. Birinci grup denekler kontrol grubu olarak de¤erlendirildi. ‹kinci grup deneklerde pinealektomi yap›larak temel endojen melatonin kayna¤› ortadan kald›r›ld› (düflük melatonin düzeyli grup). Bir hafta boyunca günde 20 mg/ml melatonin solusyonundan 0,1 ml intraperitoneal enjeksiyon yap›lan üçüncü gruptaki ratlar ise yüksek melatonin düzeyli grubu oluflturmufltur. Bu gruplar akut pankreatitin biyokimyasal parametreleri ve histopatolojik bulgular› aç›s›ndan karfl›laflt›r›larak melatonin düzeyinin akut pankreatite etkisi araflt›- r›lm›flt›r. Gruplar pankreas dokusunda polimorf nükleer lökosit infiltrasyonu, ödem, hemoraji, asiner hücre dejenerasyonu ve apoptozis gibi histopatolojik de¤ifliklikler, malonildialdehid (MDA), glutatyon (GSH) ve superoksid dismutaz (SOD) düzeyleri, biyokimyasal olarak serum aspartat transaminaz (AST), alanin transferaz (ALT), amilaz, laktat dehidrogenaz (LDH) ve bilirubin de¤erlerindeki de¤ifliklikler aç›s›ndan karfl›laflt›r›ld›. Bulgular: Yüksek melatonin düzeyinin pankreas dokusunda polimorf nükleer hücre infiltrasyonunu, ödem, hemoraji, asiner hücre dejenerasyonu, apoptozi, AST, LDH, amilaz, bilirubin ve doku MDA düzeyini azalt›c› etkisi oldu¤ u tespit edilmifltir. Sonuç: Sonuç olarak ekzojen melatonin verilmesinin, pankreasta oksidatif hasar› azaltmak suretiyle dokudaki inflamatuar reaksiyonlar› k›smen önleyici bir etkiye sahip oldu¤u kanaatine var›lm›flt›r.
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  • 3
84-92
  • Turkish

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