Buradasınız

Anasayfa » Content

PANDEMİK INFLUENZA A (H1N1) 2009 ENFEKSİYONLARININ LABORATUVAR TANISI

LABORATORY DIAGNOSIS OF PANDEMIC INFLUENZA A(H1N1) 2009 INFECTION

Journal Name:

  • İstanbul Tıp Fakültesi Dergisi

Publication Year:

  • 2010

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Fast and accurate diagnosis of influenza virus infections has become one of the most important components in clinical management and containment of current pandemic. Therefore, “real- time reverse transcription- polymerase chain reaction” (rRT-PCR)” has become the reference method for diagnosis of pandemic A (H1N1)2009 due to its ability of generating fast, and accurate results, in less than 2.5 hours. Virus isolation, direct immunofluorescence (DFA), in-direct immunofluorescence (IFA) and rapid tests are not recommended for diagnosis of pandemic A(H1N1) 2009 unless necessary. Rapid tests have been considered for use by some diagnostic laboratories due to their ease of use and generation of fast results. However, scientific reports indicate the low sensitivity as one of the drawbacks of this method and do not recommend rapid tests for use in diagnosis of pandemic A (H1N1) 2009. rRT-PCR method developed by Centers for Disease Control and Prevention and authorized by World Health Organization (WHO) has been used at Istanbul University, Istanbul Faculty of Medicine, National Influenza Reference Laboratory since the beginning of the pandemic.
Bookmark/Search this post with
Abstract (Original Language): 
Yüzyılın ilk pandemisini yaşadığımız 2009-2010 influenza mevsiminde tedavinin planlanması ve pandemi yayılımının mümkün olduğunca yavaşlatılabilmesi ancak hızlı ve doğru tanı ile mümkündür. Bu amaçla günümüzde en hızlı ve doğru tanı yöntemi olan “real- time reverse transcription- polymerase chain reaction” (rRT-PCR)” referans yöntem olarak tüm dünyada yaygın olarak kullanıma girmiştir. Öte yandan mevsimsel influenza tanısında kullanılan direkt immunofluoresan (DFA), indirekt-immunofluoresan (IFA) ve hızlı testler gibi duyarlıkları düşük testler zorunlu olmadıkça pandemik influenza enfeksiyonların tanısı için önerilmemektedir. Özellikle hızlı testler uygulamadaki kolaylıkları ve yarım saat gibi kısa bir sürede sonuç vermeleri dolayısıyla pandemi tanısında gündeme gelmiş, ancak yapılan bilimsel çalışmalar bu testlerin pandemi tanısında kullanılmasının sakıncalarını dile getirmişlerdir.İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Ulusal İnfluenza Referans Laboratuarında pandemik influenza tanısı CDC tarafından geliştirilen ve DSÖ’ nün onayladığı rRT-PCR referans yöntemiyle yapılmaktadır.
FULL TEXT (PDF): 
PDF icon arastirmax-pandemik-influenza-h1n1-2009-enfeksiyonlarinin-laboratuvar-tanisi.pdf
  • 1
27-33
  • Turkish

REFERENCES

References: 

1. Baden LR, Drazen JM, Kritek PA, Curfman GD, Morrissey S, Campion EW. H1N1
Influenza A Disease- Information for Health Professionals. N Engl J Med 2009; 360: 2666-
2667
2. Boggild AK, McGeer J. Laboratory Diagnosis of 2009 H1N1 influenza A virus. Crit Care
Med 2010; 38: S1-S5
3. Carr MJ, Gunson R, Maclean A, Coughlan S, Fitzgerald, Scully M, O’Herlihy B, Ryan J,
O’Flanagan D, Connell J, Carman WF, Hall WW. Development of a real time RT-PCR for
the detection of Swine-lineage Influenza A(H1N1) virus infections. J. Clin Virol 2009; 45:
196-199
CDC. Evaluation of rapid influenza diagnostic tests for detection of novel influenza A
(H1N1) virus. United States, 2009. MMWR 2009; 58: 826-829
4. CDC. Performance of rapid influenza diagnostic tests during two school outbreaks of 2009
pandemic influenza A (H1N1) virus infection-Connecticut, 2009. MMWR2009; 58: 1029-
1032
5. CDC. Swine Influenza A (H1N1) Infection in Two Children-Southern California, March-
April 2009. MMWR 2009; 58: 400-402
6. CDC. Outbreak of Swine-Origin Influenza A (H1N1) Virus Infection-Mexico, March-April
2009. MMWR 2009; 58: 467-470
7. CDC. Interim guidance of specimen collection, Processing and Testing for Patients with
Suspected Novel Influenza A (H1N1) Virus Infection. http://www.cdc.gov/
h1n1flu/specimencollection.htm. (Erişim, 17 Mart 2010)CDC. CDC Protocol of Real-time
RT-PCR for Influenza A (H1N1)
8. http://www.who.int/csr/resources/publications/swineflu/realtimeptpcr/en/....
(Erişim, 17 Mart, 2010)
9. Chan KH, Lai ST, Poon LLM, Guan Y, Yuen KY, Peiris JSM. Analytical sensitivity of rapid
32
influenza antigen detection tests for swine-origin influenza virus (H1N1). J Clin Virol 2009;
45: 205-207
10. Ciblak MA, Albayrak N, Odabaş Y, Altas AB, Kanturvardar M, Hasoksuz M, Sucaklı B,
Korukluoglu G, Bal E, Ertek M, Badur S. Cases of influenza A(H1N1)v reported in Turkey,
May-July 2009. Euro Surveill 2009; 14 (32): pii: 19304. [Erratum in: Euro Surveill
2009;14(33). pii: 19313]
11. Drexler JF, Hemler A, Kirberg H, Reber U, Panning MM, Hofling K, Matz B, Drosken C,
Eis-Hubinger AM. Poor clinical sensitivity of rapid antigen test for influenza A pandemic
(H1N1) 2009 virus. Emerg Infect Dis 2009;15: 1662-1664
12. Ellis J, Iturriza M, Allen R, Bermingham A, Brown K, Gray J, Brown D. Evaluation of Four
Real- Time PCR Assays For Detection of Influenza A(H1N1)v Viruses. Euro Surveill 2009:
14 (22). pii: 19230
13. Ginocchio CC, Zhang F, Manji R, Arora S, Bornfreund M, Falk L, Lotlikar M, Kowerska
M, Becker G, Korologos D, de Geronimo M, Crawford JM. Evaluation of multiple test
methods for the detection of the novel 2009 influenza A (H1N1) during the New York City
outbreak. J Clin Virol 2009; 45: 191-195
14. Hurt AC, Baas C, Deng Y-M, Roberts S, Kelso A, Barr IG. Performance of influenza rapid
point-of-care tests in the detection of swine lineage A(H1N1) influenza viruses. Influenza
Other Resp Viruses 2009; 3: 171- 174
15. Kanturvardar M, Ciblak MA, Asar S, Bozkaya E, Yenen OŞ, Badur S. Pandemik A/H1N1
Enfeksiyonlarını Tanısında Hızlı test Sorunu. Klimik Derg 2010; 22: 79-81
16. Ling LM, Chow AL, Lye DC, Tan AS, Krishnan P, Cui L, Win NN, Chan M, Lim PL, Lee
CC, Leo YS. Effects of early oseltamivir therapy on viral shedding in 2009 pandemic
influenza A (H1N1) virus infection. Clin Infect Dis 2010; 50: 963-969.
17. Mahony J, Hachette T, Orjik D, Drews SJ, Smieja M. Multiplex PCR tests sentinel the
appearance of pandemic influenza viruses including H1N1 swine influenza. J Clin Virol
2009; 45: 200-202
18. Monne I, Ormelli S, Salviato A, Battisti CD, Bettini F, Salomoni A, Drago A, Zecchin B,
Capua, I, Cattoli G. Development and Validation of a One-Step Real-Time RCR Assay for
Simultaneous Detection of Subtype H5, H7 and H9 Avian Influenza Viruses. J Clin
Microbiol 2008; 46: 1769-1773
19. NCBI, GenBank April 2009 http://www.ncbi.nlm.nih.gov/nuccore/
FJ966082.1?ordinalpos=13&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPa
nel.Sequence_RVDocSum
20. Singh K, Vasoo S, Stevens J, Schreckenberger P, Trenholme G. Pitfalls in the diagnosis of
Pandemic (Novel) A/H1N1 2009 Influenza. J Clin Microbiol 2010; 48: 1501-1503
21. Whiley MD, Bialasiewicz, Bletchly C, Faux CE, Harrower B, Gould A, Stephen L, Nimmo
GR, Nissen MD, Sloots TP. J Clin Virol 2009; 45: 203-204
22. WHO. World now at the start of 2009 influenza pandemic. http://www.
who.int/mediacentre/news/statements/2009/h1n1_pandemic_phase6_20090611/en/index.ht
ml (Eri_im: 29 Mart 2010)
WHO. WHO information for laboratory diagnosis of pandemic (H1N1) 2009 virus in
humans-revised.
http://www.who.int/csr/resources/publications/swineflu/WHO_Diagnostic_Re...
nsH1N1_20090521.pdf (Erişim: 17 Mart 2010)

Thank you for copying data from http://www.arastirmax.com