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SOMATİK HÜCRE NUKLEUS AKTARIMI-II: UYGULAMA ALANLARI

SOMATIC CELL NUCLEAR TRANSFER-II: APPLICATION AREAS

Journal Name:

  • İstanbul Üniversitesi Diş Hekimliği Fakültesi Dergisi

Publication Year:

  • 2007

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Reproductive cloning as one of the cloning studies performed by somatic cell nuclear transfer (SCNT) method, is used in production of transgenics and transgenic livestock. It is also used in protection of species which are about to extinct. Therapeutic cloning studies is rather performed to obtain patient-specific pluripotent stem cells and it aims to use these cells in cell-replacement therapy. Organ generation in human for transplantation, genetic and cpigenelic alterations during development or disease, basic research about gene expression and functions, functional male or female garnet production are some of the future goals of cloning. Producing transgenic clones, substances which can be used in treatments of various diseases is also aimed. Through cloning method, embryos of species which are extinct but their genetic structure is somehow available for us to reach, can be produced and using SCNT method development of mammal species can be observed. There are countless advantages of cloning studies, during treatment and research processes, however, this technology comes with some risks.
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Abstract (Original Language): 
Somatik hücre nukleus aklarımı yöntemiyle yapılan klonlama çalışmalarından üreme amaçlı klonlama üstün verim alınan çiftlik hayvanlarının üretimi ve araştırmalarında, transgenik canlıların üretimi, transgenic çiftlik hayvanlannın üretimi ve soyu tükenmekte olan türlerin korunmasında kullanılmaktadır. Tedavi amaçlı klonlama çalışmaları ise daha çok kişiye özgü pluripotent kök hücre elde etmeyi ve bunu hücre replasmanı tedavisinde kullanmayı amaçlar. İnsanlarda transplantasyon için organ üretilmesi, gelişim ve hastalıkta genetik vc epigenetik değişiklikler, gen ekspresyon ve işlevlerinin temci araştırmaları ve fonksiyonel erkek veya dişi gamet üretilmesi klonlamamn ileriye dönük hedefleri arasındadır. Transgenik klonlar üretilerek çeşitli hastalıkların tedavisinde kullanılabilecek bazı maddeleri elde etmek de amaçlanmaktadır. Klonlama yöntemiyle soyu tükenmiş ancak genetik yapılarına ulaşılması mümkün olan canlıların embriyoları oluşturulabilecek ve somatik hücre nukleus aktarımı yöntemiyle memelilerde bir soyun gelişimi izlenebilecektir. Klonlama çalışmalarının tedavi ve araştırma sürecinde sayılamayacak kadar çok yararlan vardır. Ancak bu teknoloji beraberinde bazı riskler de taşır.
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REFERENCES

References: 

İ. Trounson
AO
. Future and applications of cloning. Methods Mol Biol. 2006:348:319-32.
2. Kues WA. Carnwath JW. Niemann H. From fibroblasts and stem cells: implications for cell therapies and somatic cloning. Reprod Fertil Dev. 2005: 17: 125-34.
3. Paterson L, DeSousa P. Ritchie W, King T, Wilmut I. Application of reproductive biotechnology in animals: implications and potentials. Applications of reproductive cloning. Anim Reprod Sci, 2003: 79: 137-43.
4. Meissner A. Jaenisch R. Mammalian nuclear transfer. Dev Dyn, 2006: 235: 2460-9.
5. Pellestor F. Anahory T. Hamamah S. The chromosomal analysis of human oocytes. An overview of established procedures. Hum Reprod Update. 2005: 11: 15-32.
6. Berr A. Schubert I. Direct labelling of BAC-DNA by rolling-circle amplification. Plant J. 2006: 45: 857-62.
7. Anahory T. Andreo Ei. Regnier-Vigouroux Ci. Soulie JP, Baudouin M. Demaille J, Pellestor F. Sequential multiple probe fluorescence in-situ hybridization analysis of human oocytes and polar bodies by combining centromeric labelling and whole chromosome painting. Mol Hum Reprod. 2003: 9: 577-85.
8. Traut W. Eickhof U, Schorch JC. Identification and analysis of sex chromosomes by comparative genomic hybridization (CGH). Methods Cell Sci. 2001: 23: 155-61.
9. Gadji M. Krabchi K, Drouin R. Simultaneous identification of chromosomes 18, X and Y in uncultured amniocytes by using multi-primed in situ labelling technique. Clin Genet, 2005: 68: 15-22.
10. Marko JF. Siggia ED. Polymer models of meiotie and mitotic chromosomes. Mol Biol Cell, 1997: 8: 2217-31.
11. Fernandez-Capetillo O. Mahadevaiah SK. Celeste A, Romanienko PJ. Camerini-Otero RD. Bonner WM. Manova K. Burgoyne P. Nussenzweig A. H2AX is required for chromatin remodeling and inactivation of sex chromosomes in male mouse meiosis. Dev Cell. 2003: 4: 497-508.
12. Rhee K. Wolgetnuth DJ. The NIMA-related kinase 2, Nek2. is expressed in specific stages of the meiotie cell cycle and associates with meiotie chromosomes. Development. 1997: 124: 2167-77.
13. Kishimoto T. Cell cycle arrest and release in starfish oocytes and eggs. Semin Cell Dev Biol. 1998: 9: 549-57.
14. Reynaud K. Fontbonne A. Marseloo N. Thoumirc S. Chebrout M. de Lesegno CV, Chastant-Maillard S. In vivo meiotie resumption, fertilization and early embryonic development in the bitch. Reproduction. 2005: 130: 193-201.
15. Hausmann M. I.iebe B. Perner B, Jerratsch M, Greulich KO, Scherthan H. Imaging of human meiotie chromosomes by scanning near-field optical microscopy (SNOM). Micron. 2003: 34: 441-7.
16. Nagy ZP. Chang CC. Artificial gametes. Theriogenology. 2007: 67: 99-104.
17. Sakai N. Transmeiotic differentiation of zebrafish germ cells into functional sperm m culture. Development. 2002: 129: 3359-65.
Somatik Hücre Nukleus Aktarımı-II: Uygulama Alanları
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18. McLaren A. Germ cells and germ cell sex. Philos Trans R Soc Lond B Biol Sci. 1995: 350: 229-33.
19. Esa A. Edelmann P. Kreth G, Trakhtenbrot L. Amariglio N. Rechavi G. Hausmann M. Cremer C. Three-dimensional spectral precision distance microscopy of chromatin nanostructures after triple-colour DNA labelling: a study of the BCR region on chromosome 22 and the Philadelphia chromosome. J Microsc, 2000: 199: 96-105.
20. Ghosh S, Paweletz N. Detection of intranucleolar chromatin using an ultrastructural immunolabelling technique. Cell Biol Int. 1998: 22: 609-14.
21. Plusa B. Grabarek .IB. Karasiewiez J, Modlinski JA. Meiotie maternal chromosomes introduced to the late mouse zygote are recruited to later embryonic divisions. Mol Reprod Dev. 2005: 70: 429-37.
22. Bondioli K. Ramsoondar .1. Williams B, Costa C, Fodor W. Cloned pigs generated from cultured skin fibroblasts derived from a H-
transferase transgenic boar. Mol Reprod Dev, 2001: 60: 189-95.
23. Ehrenfeld D. Transgenics and vertebrate cloning as tools for species conservation. Conserv Biol, 2006: 20: 723-32.
24. Moore KA, Ema H. Lemischka IR. In vitro maintenance of highly purified, transplantable hematopoietic stem cells. Blood, 1997: 89: 4337-47.
25. Fisehbach GD, Fischbach RL. Stem cells: science, policy, and ethics. J Clin Invest, 2004: I 14: 1364-70.
26. Pittenger MF. Mackay AM, Beck SC. .laiswal RK, Douglas R. Mosca JD, Moorman MA, Simonetti DW. Craig S. Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science. 1999: 284: 143-7.
27. Reyes M. Lund T. Lenvik T. Aguiar D, Koodie L, Verfaillie CM. Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells. Blood, 2001: 98: 2615-25.

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