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ERİTİLMİŞ KOÇ SPERMASINDA FARKLI GLİSEROL KATMA TEKNİKLERİNİN VE SOĞUTMA HIZININ SPERMATOLOJİK ÖZELLİKLERE ETKİSİ

Effect of Different Glycerol Addition Techniques and Cooling Rates on Spermatological Characteristics in Thawed Ram Semen

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2007

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In the present study, effects of glycerol addition to semen at various temperatures and cooling rates of semen on the post-thaw semen characteristics on ram semen was investigated. Semen of 10 Kivircik rams of 3-5 years old were collected, pooledand frozen in straws. Six groups were established according to the extension of semen in a single step (30°C) or two steps (30°C and 5°C) and to the cooling technique to 5°C [slow (<0.2°C/min) or two (from 30°C to 16°C <2°C/min and then from 16°C to 5°C <0.2/min) stages]. Semen was extended with extender A without glycerol at 30°C and cooled to 5°C slowly (group 1) or in two-stages (group 2), at this temperature extender B was added.Again semen was extended with 2% glycerol bearing extender A at 30°C and cooledslowly (group 3) and in two-stages (group 4). The other groups were extended at 30°C with extender A and B (4% glycerol) and cooled slowly (group 5) or in two-stages (group 6). Samples were frozen in 0.25 ml straws after the equilibration. Spermatological examinations (motility, acrosomal and total morphological defect rates) were done after extension (level 1), after cooling to 5°C (level 2), after equilibration (level 3) and after thawing (level 4). In the present study, it was concluded that (1) cooling from 30°C to 5°C in the presence of glycerol at 1:1/2 dilution rate, deteriorated the post-thaw motility, (2) this deterioration was compensated by two-staged cooling technique.
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Abstract (Original Language): 
Sunulan çalışmada spermaya gliserolün farklı ısılarda eklenmesi ve spermanın farklıhızlarda soğutulmasının, koçlarda donma sonrasıspermatolojik özelliklere nasıl etkilediği araştırıldı. Üç-beşyaşlarında 10 başKıvırcık ırkıkoçun spermalarıalınarak pooling yapıldıve sulandırılarak payetlerde donduruldu. Spermanın tek aşamada (30°C) veya iki aşamada (30°C’de ve 5°C’de) sulandırılmasıve 5°C’ye yavaş (<0.2°C/dakika) ve iki-kademeli (30°C’den 16°C’ye <2°C/dakika ve 16°C’den 5°C’ye <0.2/dakika) soğutma tekniklerine göre 6 grup oluşturuldu: Sperma 30°C’de gliserol içermeyen sulandırıcıA ile sulandırıldıve 5°C’ye yavaş(1. grup) ve iki-kademeli (2. grup) soğutuldu ve B sulandırıcısıilave edildi. Yine sperma 30°C’de %2 gliserol içeren sulandırıcıA ile sulandırıldıve 5°C’ye yavaş(3. grup) ve iki-kademeli (4. grup) soğutuldu. Diğer gruplarda, sulandırma işlemi sulandırıcıA ve B ile 30°C’de tamamlandıve 5°C’ye yavaş(5. grup) ve iki-kademeli (6. grup) soğutuldu. Örnekler, ekilibrasyon sonrasıpayetlerde donduruldu. Spermatolojik incelemeler (motilite, akrozomal ve toplam morfolojik bozukluklar), sulandırma sonrası(1. aşama), 5°C’ ye soğutma sonrası(2. aşama), ekilibrasyon sonrası(3. aşama) ve eritme sonrası(4. aşama) gerçekleştirildi. Sunulan çalışmada (1) 1:1/2 sulandırma oranında 30°C’den 5°C’ye soğuma aşamasında ortamda gliserol varlığının, eritme sonrasımotiliteye zarar verdiği, (2)bu zararın iki-kademeli soğutma tekniği ile azaldığısonuçlarına varılmıştır.
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  • Turkish

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