Buradasınız

Anasayfa » Content

TAVUK ETLERİ VE TAVUKLARDAN İZOLE EDİLEN SALMONELLA ENTERİCASUBSP. ENTERİCA SEROVAR ENTERİTİDİS SUŞLARININ FAJ TİPLENDİRİLMESİVE PULSED-FİELD GEL ELECTROPHORESİS İLE ANALİZİ

Analysis of Salmonella entericasubsp. enterica Serovar Enteritidis Strains Isolated from Chickens and Chicken Meat by Pulsed-Field Gel Electrophoresis and Phage Typing

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2008

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The aim of this study was to analyse S.Enteritidis strains isolated from chickens and chicken meat by Pulsed-Field Gel Electrophoresis (PFGE) and phage typing. A total of 38 S.Enteritidis strains were analysed by PFGE after digestion of DNA with XbaI restriction enzyme. Seven different PFGE profiles were observed. A predominant type (X1) was found in 27 (71 %) of 38 S.Enteritidis strains. For all the strains, the genetic similarity was 85 %. The most common phage type was PT4, which was found in 15 (39.4 %) of the strains. Other phage types were PT7 (18.4 %), PT16 (13.1 %), PT1(10.5 %), PT6 (7.8 %) and PT35 (2.6 %). Three strains were RDNC (reacted but did notconform). Different phage types such as PT1, 4, 6 and 7 belonged to the same PFGE pattern (X1). The results of this study shows that S. Enteritidis strains originated from chickens in Turkey are genetically related. Further studies should be performed to determine genetic relationships among animal and human isolates.
Bookmark/Search this post with
Abstract (Original Language): 
Bu çalışma tavuk ve tavuk etlerinden izole edilen Salmonella entericasubsp. enterica serovar Enteritidis (S. Enteritidis) suşlarının Pulsed-Field Gel Electrophoresis (PFGE) ve Faj tiplendirme yöntemleriyle tiplendirilmesi amacıyla yapıldı. Toplam 38 S.Enteritidis suşu XbaI restriksiyon enzimi kullanılarak PFGE yöntemiyle tiplendirildi.Tiplendirme sonucunda 7 farklıbant profili elde edildi. Analiz edilen 38 S. Enteritidis suşunun 27 sinde (%71) aynıPFGE bant profili (XI) saptandı. Tüm suşlar arasındaki genetik benzerlik % 85 bulundu. Faj tiplendirme sonucunda 38 suşun 15’i (% 39.4) S. Enteritidis PT4 (Faj Tip 4) olarak tiplendirildi. Suşların % 18.4’i PT7, % 13.1’i PT16, % 10.5’i PT1, % 7.8’i PT6 ve % 2.6’sıPT35 faj tipinde bulundu. Suşların 3’ünün faj tipi belirlenemedi. Farklıfaj tipindeki (PT1, 4, 6 ve 7) suşlarda aynı PFGE profili (X1) saptandı. Bu çalışmanın sonuçlarıTürkiye’de tavuk orijinli S. Enteritidis suşlarıarasında bir genetik ilişkinin olduğunu göstermektedir. İnsan ve hayvan izolatlarıarasındaki genetik ilişkiyi ortaya koymak için ilave çalışmalara ihtiyaç duyulmaktadır.
FULL TEXT (PDF): 
PDF icon arastirmax-tavuk-etleri-tavuklardan-izole-edilen-salmonella-entericasubsp.enterica-serovar-enteritidis-suslarinin-faj-tiplendirilmesive-pulsed-field-gel-electrophoresis-ile-analizi.pdf
  • 1
15-24
  • Turkish

REFERENCES

References: 

1. Anğ-Küçüker, M., Tolun, V., Helmuth, R., Rabsch, W., Büyükbaba-Boral, Ö.,
Törümküney-Akbulut, D., Susever, S., Anğ, Ö.:Phage types, antibiotic susceptibilities
and plasmid profiles of Salmonella typhimuriumand Salmonella enteritidisstrains isolated in
Istanbul, Turkey. Clin. Microbiol. Infect., 2000; 6(11): 593-599.
2. Anonim: Salmonellosis control: The role of animal and product hygiene. WHO Expert
Committee, Technical Report Series. 774. 1988.
3. Bekar, M., Ayaz, Y., Akman, A., Yazıcıoğlu, N., Uysal, Y., Tekin, C., Korkut, N.,
Mirioğlu, M., Aslan, A., Ergün, A., İldeş, Z.:Tavuk mezbahalarının Salmonella yönünden
taranması. Etlik Vet. Mikrobiyol. Derg., 1993; 7(4):1-23.
4. Ben Aissa, R., Al-Gallas, N.:Molecular typing of Salmonella entericaserovars Enteritidis,
Corvallis, Anatum and Typhimurium from food and human stool samples in Tunisia, 2001-2004. Epidemiol. Infect., 2008; 136(4): 468-475.
5. Betancor, L., Schelottto, F., Martinez, A., Pereira, M., Algorta, G., Rodríguez, M.A.,
Vignoli, R., Chabalgoity, J.A.:Random amplified polymorphic DNA and phenotyping
analysis of Salmonella entericaserovar Enteritidis isolates collected from humans and
poultry in Uruguay from 1995 to 2002. J. Clin. Microbiol., 2004; 42(3):1155-1162.
6. Boonmar, S., Bangtrakulnonth, A., Pornrunangwong, S., Terajıma, J., Watanabe, H.,
Kaneko, K., Ogawa, M.:Epidemiological analysis of Salmonella enteritidis isolates from
humans and broiler chickens in Thailandby phage typing and pulsed-field gel
electrophoresis. J. Clin. Microbiol., 1998; 36(4): 971-974.
7. Cardinale, E., Perrier Gros-Claude, J.D., Rivoal, K., Rose, V., Tall, F., Mead, G.C.,
Salvat, G.:Epidemiological analysis of Salmonella entericassp. Enterica serovars Hadar,
Brancester and Enteritidis from humans and broiler chickens in Senegal using pulsed-field
gel electrophoresis and antbiotic susceptibility. J. Appl. Microbiol., 2005; 99(4):968-977.
8. Cheong, H.J., Lee, Y.J., Hwang, I.S., Kee, S.Y., Cheong, H.W., Song, J.Y., Kim, J.M.,
Park, Y.H., Jung, J.H., Kim, W.J:Characteristics of non-typhoidal Salmonella isolates
from human and broiler chickens in Southwestern Seoul, Korea. J. Korean Med. Sci., 2007;
22(5): 773-778.
9. Çarlı, K.T., Eyigör, A., Caner, V.: Prevalence of Salmonella serovars in chickens in
Turkey. J. Food Microbiol., 2001; 64(11):1832-1835.
10. De Jong, B., Ekdahl, K.:The comparative burden of salmonellosis in the European Union
member states, associated and candidate coutries. BMC Publich Health, 2006; 6:4.
11. Durmaz, B., Durmaz, R.: Pulsed-Field Gel Electrophoresis. Erişim 13.05.2008, İnönü
Üniversitesi, Tıp Fakültesi. http://web.inonu.edu.tr/~iozerol/rdurmaz/ UygMolMikr/161.pdf
12. Durmaz, R., Otlu, A., Çalışkan, A., Gürsoy, N.: Acinetobacter baumannii, Escherichia
colive Klebsiellatürleinin moleküler tiplendirilmesinde kullanılabilecek kısa süreli “pulsed
field gel” elektroforez (PFGE) protokolü. ANKEM Derg., 2007; 21(2):113-117.
13. Erdem, B., Ercis, S., Hasçelik, G., Gür, D., Gedikoğlu, S., Aysev, A.D., Sümerkan, B.,
Tatman-Otkun, M., Tuncer, İ.:Antimicrobial resistance patterns and serotype distribution
among Salmonella entericastrains in Turkey. 2000-2002. Eur. J. Clin. Microbiol. Infect.
Dis., 2005; 24(3):220-225.
14. Fernandez, J., Fica, A., Ebensperger, G., Calfullan, H., Prat, S., Fernandez, A.,
Alexandre, M., Heitmann, I.:Analysis of molecular epidemiology of Chilean Salmonella
entericaserotype Enteritidis isolates by pulsed-field gel electrophoresis and bacteriophage
typing. J. Clin. Microbiol., 2003; 41(4):1617-1622.
15. Hasenson, L.B., Kaftyreva, L.,Laszlo, V.G., Woitenkova, E., Nesterova, M.:
Epidemiological and microbiological data on Salmonella enteritidis. Acta Microbiol. Hung.,
1992; 39(1):31-39.
16. Hickman-Brenner, F.W., Stubbs, A.A., Farmer, J.J.:Phage typing of Salmonella
enteritidisin the United States. J.Clin.Microbiol., 1991; 29(12):2817-2823.
17. Kalender, H., Muz, A.:ElazığBölgesindeki tavuklardan izole edilen Salmonella türlerinin
tiplendirilmesi. Tr. J. Vet. Anim. Sci., 1999; 23(2):297-303.
18. Kılınç, Ü., Aydın, F.:Kayseri yöresindeki tavukçuluk işletmelerinden toplanan tavuklardan
izole edilen Salmonella türlerinin antibiyotiklere duyarlılıkları. Erciyes Üniv. Sağ.Bil.Derg.,
2006; 15(1): 35-40.
19. Laconcha, I, Baggesen, D.L., Rementeria, A., Garaizar, J.: Genotypic characterisation by
PFGE of Salmonella enterica serotype phage types 1, 4, 6 and 8 isolated from animal and
human sources in three European countries. Vet. Microbiol., 2000; 75(2): 155-165.
20. Liebana,, E., Garcia-Migura, L., Breslin, M.F., Davies, R.H., Woodward, M.J.:
Diversity of strains of Salmonella entericaserotype Enteritidis from English poultry farms
assessed by multiple genetic fingerprinting.J. Clin. Microbiol., 2001; 39(1):154-161.
21. Liebana, E., Garcia-Migura, L., Guard-Petter, J., McDowell, S.W.J., Rankin, S., Opitz,
H.M., Clifton-Hadley, F.A., Davies, R.H.: Salmonella entericaserotype Enteritidis phage
types 4, 7, 6, 8, 13a, 29 and 34: a comparative analysis of genomic fingerprints from
geographically distant isolates. J. Appl. Microbiol., 2002; 92(2):196-209.
22. Liebana, E., Clouting, C., Garcia-Migura, L., Clifton Hadley, F.A., Lindsay, E.,
Threlfall, E.J., Davies, R.H.:Multiple genetic typing of Salmonella enteritidis phage-types
4, 6, 7, 8 and 13 isolates from animals and humans in the UK. Vet. Microbiol., 2004; 100(3-4):189-195.
23. Ling, J.M., Koo, I.C., Kam, K.M., Cheng, A.F.:Antimicrobial susceptibilities and
molecular epidemiology of Salmonella enterica serotype Enteritidis strains isolated in Hong
Kong from 1986 to 1996. J. Clin. Microbiol., 1998; 36(6):1693-1699.
24. Lopes, V.C., Velayudhan, B.T., Halvorson, D.A., Lauer, D.C., Gast, R.K., Nagaraja,
K.V.:Comparison of methods for differantion of Salmonella entericaserovar Enteritidis
phage type 4 isolates. Am. J.Vet.Res., 2004; 65(5): 538-543.
25. Mutluer, B., Yargülü, B., Hartung, M., Erol, İ.:Incidence and serovar distribution of
Salmonella in market broilers in Turkey. 3 rd. World Congress Foodborne Infectious and
Intoxications. Proceedings. Vol. II. 16-19 June , Berlin, 1992, 1075-1079.
Hakan KALENDER- Selahattin ŞEN 24
26. Pang, J.C., Chiu, T.H., Chiou, C.S. Schroeter, A., Guerra, B., Helmuth, R., Tsen,
H.Y.:Pulsed-field gel electrophoresis, plasmid profiles and phage types for human isolates
of Salmonella enterica serovar Enteritidis obtained over 13 years in Taiwan. J. Appl.
Microbiol., 2005; 99(6):1472-1483.
27. Ribot, E.M., Wierzba, R.K., Angulo, F.J., Barrett, T.J.: Salmonella entericaserotype
Typhimurium DT104 isolated from humans, United States, 1985, 1990, and 1995. Emerg.
Infect. Dis., 2002; 8(4):387-91.
28. Ridley, A.M., Therelfall, E.J., Rowe, B.:Genotypic characterization of Salmonella
enteritidis phage types by plasmid analysis, ribotyping and pulsed-field gel electrophoresis.
J. Clin. Microbiol.,1998; 36(8):2314-2321.
29. Rodrigue, D.C., Tauxe, R.V., Rowe, B.:International increase in Salmonella enteritidis:a
new pandemic? Epidemiol. Infect., 1990; 105(1): 21-7.
30. Tsen, H.Y., Lin. J.S.:Analysis of Salmonella enteritidis strains isolated from food-poisoning cases in Taiwan by pulsed field gel electrophoresis, plasmid profile and phage
typing. J. Appl. Micobiol., 2001; 91(1):72-79.
31. Ward, L.R., De Sa, J. D., Rowe, B.:A phage-typing scheme for Salmonella enteritidis.
Epidemiol.Infect., 1987; 99(2):291-294.
32. Wiesner, M., Hidalgo, M., Castenade, E., Agudelo, C.I.:Malecular analysis of Salmonella
Enteritidis and Typhimurium clinical and food isolates by pulsed-field gel electrophoresis in
Bogota, Colombia. Microb. Drug Resist., 2006; 12(1): 68-73.
33. WHO :Global Salm- Surv, Laboratory protocols,Level 5 training course, pulsed field gel
electrophoresis for Salmonella. 2005.

Thank you for copying data from http://www.arastirmax.com