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Boynuzlu Bir Keçide Freemartinizmin Hormonal ve Tekli Multipleks PCR Analiz Uygulamaları ile Tespiti

Application of Hormonal and Single Multiplex PCR Assays for Detection of Freemartinism in a Horned Goat

Journal Name:

  • İstanbul Üniversitesi Veteriner Fakültesi Dergisi

Publication Year:

  • 2012

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The present study describes a rare case of freemartinism of the 60XX/60XY chimera type in an adult 7-year-old horned, mixed Lori breed phenotypically doe. Signs of masculinisation were evident in both the behavioral patterns and body conformation, but there was no structure similar to penis and hypospadias was observed in this goat. External genital organs were similar to that of a female one. In this study, a single multiplex PCR assay was used to determine the freemartinism status (XX-XY blood chimerism) based on semi-quantitatively co amplify a sex-based polymorphism in the amelogenin locus (AMX and AMY). In addition, serum concentrations of anti-Müllerian hormone (AMH), testosterone and progesterone hormones of this horned intersex goat were evaluated. Serum AMH levels in the present case (0.2 ng/ml) were similar to male (0.2 ng/ml) and lower than female (0.6 ng/ml) goat. The serum concentrations of testosterone (0.2 ng/ml) were remarkably high in comparison to normal control male and female ones (0.02 ng/ml). Progesterone level in the intersex goat (0.4 ng/ml) was close to control buck (0.7 ng/ml) and remarkably lower than control doe (10 ng/ml). The results of the present study showed that the use of hormonal pattern, especially AMH level, and single multiplex PCR technique provide an easy and alternative approaches for indication of XX/XY chimerism or mosaicism in intersex freemartin goats
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Abstract (Original Language): 
Bu çalışmada, nadir bir olgu olan freemartinizmin dişi fenotipine sahip Lori ırkı melezi 7 yaşındaki boynuzlu ergin bir keçide 60XX/XY kimera tipi bildirilmektedir. Davranış biçimlerinde ve vücut yapısında maskülinizasyon belirtileri bulunur iken, penis veya hypospadias gözlenmemiştir. Dış genital organlar dişi keçi ile benzerlik göstermektedir. Bu çalışmada amelogonin lokusundaki (AMX ve AMY) cinsiyete bağlı polimorfizmin semi-kantitatif olarak çoğaltılması ile freemartinizm durumunun (XX-XY kan kimerizmi) belirlenmesinde tekli multipleks PCR yöntemi kullanılmıştır. Ayrıca anti-Müllerian hormon (AMH), testesteron ve progesteron hormonlarının serum konsantrasyonları da değerlendirmeye alınmıştır. Mevcut vakadaki serum AMH düzeyleri (0,2 ng/ml) tekeye benzer (0,2 ng/ml) olarak, dişi keçiden (0,6 ng/ml) ise düşük olarak saptanmıştır. Serum testesteron konsantrasyonu (0,2 ng/ml) kontrol tekeye ve dişi keçiye (0,02 ng/ml) göre önemli düzeyde yüksek bulunmuştur. İnterseks keçide progesteron düzeyi (0,4 ng/ml) kontrol tekedeki düzeye (0,7 ng/ml) yakın, kontrol dişi keçideki düzeyden (10 ng/ml) ise belirgin olarak düşüktür. Mevcut çalışmanın sonuçları interseks freemartin keçilerde XX/XY kimerizmi veya mozaizmini belirlemede AMY düzeyi ve tekli multipleks PCR tekniğinin kolay ve alternatif bir yöntem olduğunu ortaya koymaktadır.
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  • 2
175-181
  • English

REFERENCES

References: 

Basrur, P.K., Kochhar, H.S., 2007. Inherited Sex
Abnormalities in Goats. In: Youngquist, R.S.,
Threlfall, W.R. (Eds.), Current Therapy in
Large Animal Theriogenology, 2
nd
Ed.
Saunders, USA, pp. 590-594.
Batista, M., Gonzalez, F., Cabrera, F., Palomino, E.,
Castellano, E., Calero, P., Gracia, A., 2000.
True hermaphroditism in a horned goat with
6OXX/6OXY chimerism. Canadian Veterinary
Journal 41, 562-564.
Behringer, R.R., 1994. The in vivo roles of müllerian-inhibiting substance. Current Topics in
Developmental Biology 29, 171-187.
Bongso, T.A., Thavalingam, M., Murkherjee, T.K.,
1982. Intersexuality associated with XX/XY
mosaicism in horned goat. Cytogenetics and
Cell Genetics 34, 315-319.
Bosu, W.T.K., Basrur, P.K., 1984. Morphological and
hormonal features of an ovine and a caprine
intersex. Canadian Journal of Comparative
Medicine 48, 402-409.
Cate, R.L., Mattaliano, R.J., Hession, C., Tizard, R.,
Farber, N.M., Cheung, A., Ninfa, E.G., Frey,
A.Z., Gash, D.J., Chow, E.P., 1986. Isolation
of the bovine and human genes for Mullerian
inhibiting substance and expression of the
human gene in animal cells. Cell 45, 685-698.
Christensen, B.W., Drost, M., Troedsson, M.T.,
2009. Diseases of the Reproductive System. In:
Smith, B.P. (Ed.), Large Animal Internal
Medicine, 4
th
Ed. Elsevier, pp. 1427-1470.
Cole, J., Broadwell, M., Rogers, G., 1997.
Intersexuality in a Charolais heifer. Veterinary
Record 141, 656-657.
Cribiu, E.P., Chaffaux, S., 1990. Intersexuality in
domestic mammals. Reproduction Nutrition
Development Supplement 1, 51-61.
Dunn, H.O., Johnson, R.H., 1981. Sample size for
detection of Y chromosomes in lymphocytes of
possible freemartins. Cornell Veterinary
Medicine 71, 297-304.
Ennis, S., Gallagher, T.F., 1994. A PCR-based sex-determination assay in cattle based on the
bovine amelogenin locus. Animal Genetics 25,
425-427.
Ennis, S., Vaughan, L., Gallagher, T.F., 1999. The
diagnosis of freemartinism in cattle using sex-specific DNA sequences. Research in
Veterinary Science 67, 111-112.
Fujishiro, A., Kawakura, K., Miyake, Y.I., Kaneda,
Y., 1995. A fast, convenient diagnosis of the
bovine freemartin syndrome using polymerase
chain reaction. Theriogenology 43, 883-891.
Hafez, S.A., Huckle, W.R., Caceci, T., 2005.
Anatomical, histological and genetic
investigations of a sexually anomalous goat.
Veterinary Record 157, 513-516.
Kevenaar, M.E., Meerasahib, M.F., Kramer, P., van
de Lang-Born, B.M., de Jong, F.H., Groome,
N.P., Themmen, A.P., Visser, J.A., 2006.
Serum anti-mullerian hormone levels reflect the
size of the primordial follicle pool in mice.
Endocrinology 147, 3228-3234.
McNiel, E.A., Madrill, N.J., Treeful, A.E., Buoen,
L.C., Weber, A.F., 2006. Comparison of
cytogenetics and polymerase chain reaction
based detection of the amelogenin gene
polymorphism for the diagnosis of
freemartinism in cattle. Journal of Veterinary
Diagnostic Investigation 18, 469-472.
Monniaux, D., Baril, G.R., Laine, A.L., Jarrier, P.,
Poulin, N., Cognié, J., Fabre, S., 2011. Anti-Müllerian hormone as a predictive endocrine
marker for embryo production in the goat.
Reproduction 142, 845-854.
Padula, A.M., 2005. The freemartin syndrome: an
update. Animal Reproduction Science 87, 93-109.
Rico, C., Fabre, S., Médigue, C., di Clemente, N.,
Clément, F., Bontoux, M., Touzé, J.L.,
Dupont, M., Briant, E., Rémy, B., Beckers,
J.F., Monniaux, D., 2009. Anti-mullerian
hormone is an endocrine marker of ovarian
gonadotropin-responsive follicles and can help
to predict superovulatory responses in the cow.
Biology of Reproduction 80, 50-59.
Ricordeau, G., 1981. Genetics: Breeding Plans. In:
Gall, C. (Ed.), Goat Production, New York,
Academic Press.
Rota, A., Ballarin, C., Vigier, B., Cozzi, B., Rey, R.,
2002. Age dependent changes in plasma anti-Müllerian hormone concentrations in the bovine
male, female, and freemartin from birth to
puberty. General and Comparative
Endocrinology 129, 39-44.
Saba, N., Cunningham, N.F., Millar, P.G., 1975.
Plasma progesterone, androstenedione and
testosterone concentrations in freemartin
heifers. Journal of Reproduction and Fertility
45, 37-45.
Seguin, B.E., Zhang, T., Buoen, L.C., Weber, A.F.,
Ruth, G.R., 2000. Cytogenetic survey of
Holstein bulls at a commercial artificial
insemination company to determine the
prevalence of bulls with centric fusion and
chimeric anomalies. Journal of the American
Veterinary Medical Association 216, 65-67.
Smith, M.C., Dunn, H.O., 1981. Freemartin condition
in a goat. Journal of the American Veterinary
Medical Association 178, 735-737.
Szatkowska, I., Zych, S., Udala, J., Dybus, A.,
Aszczyk, P.B., Sysa, P., Dabrowski, T., 2004.
Freemartinism: Three cases in goats. Acta
Veterinaria Brno 73, 375-378.
van Rooij, I.A., Broekmans, F.J., te Velde, E.R.,
Fauser, B.C., Bancsi, L.F., de Jong, F.H.,
Themmen, A.P., 2002. Serum anti-Mullerian
hormone levels: a novel measure of ovarian
reserve. Human Reproduction 17, 3065-3071.
Visser, J.A., de Jong, F.H., Laven, J.S., Themmen,
A.P., 2006. Anti-Mullerian hormone: a new
marker for ovarian function. Reproduction 131,
1-9.
Yadav, B.R., 1988. Freemartinism in goat. Indian
Journal of Animal Science 58, 123-126.

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