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Kaz (Anser anser) Özofagus Bezlerindeki Glikokonjugatların Histokimyasal Özellikleri

The Histochemical Features of Glycoconjugates in Esophageal Glands of Goose (Anser anser)

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Abstract (2. Language): 
The aim of this study to determine the glycoconjugates features of esophageal glands of goose (Anser anser) by conventional and lectin histochemical techniques. For this purpose, the samples taken from thoracal region of esophaguses of 5 adult geese were used. It was shown that there was strongly sulphated, sulphate estered, acidic and neutral glycoconjugates in epithelial cells of glands and excretory ducts. WGA, PNA, DBA, BSA I-B4, Con A and UEA I reactive sugar residues were detected in the glycoconjugates of glands epithelial cells. However there were no glycoconjugates showing specificity against the DBA, BSA I-B4, Con A and UEA I in epithelial cells of excretory ducts
Abstract (Original Language): 
Bu çalışmada kaz (Anser anser) özofagus bezlerine ait glikokonjugat özelliklerinin klasik ve lektin histokimyasal yöntemlerle belirlenmesi amaçlandı. Bu amaçla 5 adet erişkin kaz özofagusunun torakal bölgesinden örnekler alındı. Bez ve kanal epitel hücrelerinde güçlü sülfatlı, sülfat esterli, asidik ve nötral glikokonjugatların bulunduğu gözlendi. Bez epitel hücrelerindeki glikokonjugatlarda WGA, PNA, DBA, BSA I-B4, Con A ve UEA I reaktif şeker rezidüleri tespit edildi. Buna karşın kanal epitel hücrelerinde DBA, BSA I-B4, Con A ve UEA I’e karşı spesifite gösteren glikokonjugatlara rastlanmadı.
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REFERENCES

References: 

Abdulnour-Nakhoul, S., Nakhoul, N.L., Wheeler, S.A.,
Haque, S., Wang, P., Brown, K., Orlando, G.,
Orlando, R.C. 2007. Characterization of Esophageal
Submucosal Glands in Pig Tissue and Cultures. Dig.
Dis. Sci., 52, 3054-3065.
Arul, G.S., Moorghen, M., Myerscough, N., Alderson,
D.A., Spicer, R.D., Corfield, A.P. 2000. Mucin Gene
Expression in Barett’s Oesophagus: An in situ
Hybridization and Immunohistochemical Study.
Gut, 47, 753–761.
Brooks, S.A., Dwek, M.V., Schumacher, U. 2002.
Functional and Molecular Glycobiology. BIOS
Scientific Publishers Ltd., Oxford, UK, 256 p.
Danguy, A., Lenget, G., Kiss, Jr.R., De Launoit, Y., Na
Kango, M.L., Kiss, Sr.R. 1987. Distribution of
Fluorochrome-Coupled Lectins in The
Gastrointestinal Tract African Bats. Archives of
Biology, 143-148.
Dekker, J., Rossen, J. Büller, H., Einhard, A. 2002. The
MUC Family: An Obituary. Trends in Biochemical
Sciences, 27 (3): 126-31.
Dixon, J., Strugala, V., Griffin, S.M., Welfare, M.R.,
Dettmar, P.W., Allen, A., Pearson, J.P. 2001.
Esophageal Mucin: An Adherent Mucus Gel Barrier
is Absent in The Normal Esophagus But Present in
Columnarlined Barrett’s Esophagus. Am. J.
Gastroenterol., 96, 2575–2583.
Ferri, D., Liquori, G.E., Natale, L., Santarelli, G.,
Scillitani, G. 2001. Mucin Histochemistry of The
Digestive Tract of The Red-legged Frog Rana
aurora aurora. Acta Histochem., 103 (2): 225-37.
Gad, A. 1969. A Histochemical Study of Human
Alimentary Tract Mucosubstances in Health and
disease in Normal and Tumors. British Journal of
Cancer, 23 (1): 52-63.
Gheri, G., Gheri Bryk, S., Sgambati, E., Gulisano, M.
1993. Characterization of The Glycoconjugate Sugar
Residues in Developing Chick Esophageal
Epithelium. Histol. Histopathol., 8 (2): 351-8.
Goldstein, I.J., Hughes, R.C., Monsigny, M., Osawa, T.,
Sharon, I.J. 1980. What Should Be Called A Lectin?
Nature, 285, 66.
Hodges, R.D. 1974. The Histology of the Fowl. San
Francisco Academic Press, New York.
Kum, Ş. 2002. Broylerlerde Dil ve Özofagus-
Proventrikulus Arası Bölge Üzerinde Histolojik ve
Histokimyasal Çalışmalar. A.Ü. Vet. Fak. Derg., 49,
165–171.
Lev, R., Spicer, S.S. 1964. Specific Staining of Sulphate
Groups with Alcian Blue at Low pH. J. Histochem.
Cytochem., 12, 309.
Liquori, G.E., Scillitani, G., Mastrodonato, M., Ferri, D.
2002. Histochemical Investigations on The
Secretory Cells in The Oesophagogastric Tract of
The Eurasian Green Toad Bufo viridis. The
Histochemical Journal, 34, 517-524.
Mathews, C.K., Van Holde, K.H., Ahern, K.G. 2000.
Biochemistry. Benjamin Cummings, Virginia, 1186 p.
Mowry, R.W. 1956. Alcian Blue Techniques For The
Histochemical Study of Acidic Carbohydrates. J.
Histochem. Cytochem., 4, 407–408.
Nabipour, A., Raji, A.R., Basami, M.R., Babazade, M.
2009. A Comparative Study on The Histological And
Histochemical Features of Esophagus in Ostrich Chick.
Journal of Veterinary Research, 64 (4): 297-300.
Özer, A. 2008. Sindirim sistemi II: Sindirim Kanalı.
(Veteriner Özel Histoloji, Nobel Yayınevi, Ankara:
Ed. Yörük, M.) 161.
Pastor, L.M., Ballesta, J., Madrid, J.F., Perez-Tomas,
R., Hernandes, F. 1988. A Histochemical Study of
The Mucins in The Digestive Tract of The Chicken.
Acta Histochem., 83, 91-97.
Poorkhalkali, N., Jacobson, I., Helander, H.F. 1999.
Lectin Histochemistry of The Esophagus in Several
Mammalian Species. Anat. Embryol., 200, 541–549.
Sağsöz, H., Liman, N. 2009. Structure of The
Oesophagus And Morphometric, Histochemical-
Immunohistochemical Profiles of The Oesophageal
Gland During The Post-Hatching Period of Japanese
Quails (Coturnix coturnix japonica). Anat. Histol.
Embryol., 38, 330-340.
Shibata, T., Imai, M., Moroguchi, K., Takada, Y.,
Hayama, H. 1991. Actual Characteristics of The
Glands Distributed in The Lamina Propria Mucosae
of The Fowl Esophagus. Okajimas Folia Anat. Jpn.,
68, 45–50. Spicer, S.S., Mayer, D.R. 1960. Aldehyde
Fuchsin/Alcian Blue. (Cellular Pathology
Technique, Butterworths, London: Eds. Culling,
C.F.A., Allison, R.T., Barr, W.T.) 233.
Srisai, D., Juntaravimol, S., Pongkete, P., Koonjaenok,
S., Suprasert, A. 2002. Histological and
Histochemical Studies on Esophagus of The
Germain’s Swiftlet (Collocalia germani Oustalet,
1878). Kasetsart Vet., 12, 16–21.
Suprasert, A., Fujioka, T. 1987. Lectin Histochemistry of
Glycoconjugates in Esophageal Mucous Gland of The
Chicken. Nippon. Juigaku. Zasshi., 49 (3): 555-7.
Zatta, P., Zambenedetti, P. 2000. Lectins, Microglia
And Alzheimer’s Desease. (Lectins and Pathology,
Harwood Academic Publishers, Netherlands: Eds.
Caron, M., Seve, A.P.) p 216.
Zheng, T., Peelen, D., Smith L.M. 2005. Lectin Arrays
for Profiling Cell Surface Carbohydrate Expression.
Journal of the American Chemical Society, 127,
9982-9983.

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