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FARELERDE MİKRONUKLEUS TESTİ KULLANILARAK GAMA RADYASYONU VE AĞIR METAL İYONLARI TARAFINDAN TEŞVİK EDİLEN GENOTOKSİSİTENİN BELİRLENMESİ: PATOLOJİK DEĞERLENDIRMELI

DETECTION OF GENOTOXICITY INDUCED BY HEAVY METAL IONS AND GAMMA RADIATION USING MICRONUCLEUS ASSAY IN MICE: PATHOLOGICAL EVALUATION

Journal Name:

  • Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi

Publication Year:

  • 2010

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Micronuclei (MN) test is used as markers of radiosensitivity or chemosensitivity. In present study, it was investigated the frequency of MN in erythrocytes and body weight gain in 80 Mus musculus var. albinos exposed to 10 Gy gamma (γ) radiation and heavy metal ions. For this aim, it was used MN assay as an indicator of genotoxicity induced by γ–radiation and heavy metal toxication. The animals were divided into four groups: control, radiation, Hg and Pb treatment groups. They were treated with three dose levels (10, 15 and 20 μg/mL) of Hg and Pb metal ions and 10 Gy γ–radiaton was applied twice during 14 days. The initial and final weights of all mice were determined by sensitive balance in order to investigate the effect of heavy metal ions and radiation on the weight gain of mice. As a result, the frequency of MN was higher in the Hg, Pb and γ–radiation treated animals than animals in control group. Besides, MN frequency was higher in mice exposed to γ–radiation than in Hg and Pb treated mice, and differences was statistically significant (p<0.05). Histopathologically, periaciner necroses, hydropic degenerations in the liver and villous atrophy in the intestine, gastric glandular mucosae necroses were observed in treatment groups. These results indicate that MN assay is very sensitive and a useful biomarker for the evaluation of the genotoxicity.
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Abstract (Original Language): 
Mikronukleus (MN) testi, radyasyona veya kimyasallara duyarlılığın belirteci olarak kullanılmaktadır. Bu çalışmada, ağır metal iyonları ve 10 Gy gama radyasyonuna maruz kalan 80 adet Mus musculus var. albinos’da, eritrositlerde MN sıklığı ve vucüt ağırlığı kazanımı araştırılmıştır. Bu amaçla, γ–radyasyonu ve ağır metal toksikasyonu tarafından uyarılan genotoksisitenin indikatörü olarak MN testi kullanılmıştır. Hayvanlar kontrol, radyasyon, Hg ve Pb uygulama grupları olarak dört gruba ayrılmıştır. Bu gruplar ayrı ayrı Hg ve Pb metal iyonlarının üç dozuna (10, 15 ve 20 μg/mL) ve 14 gün süresince iki kez 10 Gy γ–radyasyona maruz bırakılmıştır. Farelerin ağırlık kazanımları üzerine ağır metal iyonları ve radyasyonun etkilerini araştırmak için, tüm farelerin başlangıç ve son ağırlıkları hassas terazi yardımıyla belirlenmiştir. Sonuçta MN sıklığı Hg, Pb ve γ–radyasyonu ile muamele edilen hayvanlarda, kontrol grubundakilerden daha yüksek bulunmuştur. Ayrıca, MN sıklığının, γ– radyasyonuna maruz kalan farelerde, Hg ve Pb ile muamele edilen farelerden daha yüksek olduğu bulunmuştur ve farklar istatistiksel olarak önemlidir (p<0.05). Histopatolojik olarak ise, uygulama gruplarında karaciğer hepatositlerinde hidropik dejenerasyon, periasiner nekroz, barsakta villus atrofisi ve mide glandular mukoza nekrozları gözlenmiştir. Bu sonuçlar, MN testinin radyasyon ve ağır metal iyonlarının etkilerini değerlendirmek için çok hassas ve faydalı bir biyolojik belirteç olduğunu göstermiştir.
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