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Sıçanlarda ovarektominin ve ovarektomi sonrası uygulanan östrojenin pineal bez üzerine etkisi: Işık mikroskobik çalışma

Effect of ovariectomy and ovarîectomy followed by administration of estrogen on the pineal gland: A light microscopic study.

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2002

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Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
This study was aimed to examine the effects of ovariectomy and ovariectomy followed by estrogen administration on the pineal gland by light microscopy. For this purpose 15 female Wistar rats were used. Animals were divided İnto three groups. Group I and II were designated as sham-ovariectomised (Control) and ovariectomised, respectively. They received sesame oil (0.1 mi subcutaneously) alone. The rats in Group III were ovariectomised and daily injected with Estradiol Benzoate (0.5 mg/0.1 ml sesame oil per day s.c) for 1 months. At the end, ali animals were killed by vascular perfusion. The pineal glands of rats were removed, then processed for light microscopy. Ovariectomy caused hypertrophy in pinealocytes and an İncrease of lipid droplets in the structure of pineal gland. İt was observed that Estradiol administration followİng ovariectomy inhibited ovariectomy induced hypertrophy and increase of lipid droplets. Additionally, the connective tissue betvveen parenchymal ceils was increased in this group. İn conclusion, increased of celi activity was seen in the pinealocytes after ovariectomy and this increase was suppressed following the administration of Estradiol benzoate.
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Abstract (Original Language): 
Bu çalışma, ovarektominin ve ovarektomi sonrası uygulanan östrojenin pineal bez üzerine etkisinin ışık mikroskop düzeyde araştırılması amacıyla yapıldı. Bu amaçla 15 adet Wistar-Albino cinsi dişi sıçan kullanıldı. Hayvanlar üç gruba ayrıldı. Grup I ve Grup II sırasıyla kontrol (Sham-ovarektomİ) ve ovarektomili sıçanlar olarak düzenlendi. Bu hayvanlara günlük olarak ve derialtı yolla 0.1 mi susamyağı enjekte edildi. Grup III deki sıçanlara da ovarektomi sonrası günlük olarak ve derialtı yolla 0.1 ml susam yağı içerisinde 0.5 mg Estradiol Beonzoate enjekte edildi. Bir aylık deney süresi sonunda tüm hayvanlar vasküler perfüzyonla öldürüldü. Sıçanların pineal bezleri çıkartılarak rutin histolojik yöntemlerle ışık mikroskobik preparatları hazırlandı. Çalışmamızda, ovarektomi sonrası pinealositierde hipertrofinin oluştuğu ve bez yapısında lipid damlacıklarında artış meydana geldiği gözlendi. Ovarektomi sonrası östrojen enjeksiyonu sonucunda ise, ovarektomi sonrası gözlenen hipertrofinin ve lipid damlacıklarındaki artışın kaybolduğu tespit edildi. Ayrıca, parankima! hücreler arasındaki bağ dokuda artış gözlendi. Sonuç olarak, ovarektomi sonrası pinealosit hücre aktivasyonunda artış meydana geldiği ve bu artışın östrojen enjeksiyonu ile baskılandığı görüldü.
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