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Sıçan testisinde sigara maruziyeti ile oluşan histolojik değişiklikler üzerine kafeik asit fenetil ester (CAPE)'in koruyucu etkisi

Protective effects of caffeic acîd phenetyf ester (CAPE) on exposure of cigarette smoke-lnduced histolo-gical changes in rat testes

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
The aim of this study was to investigate the histological changes in testis of rats exposed to cigarette smoke and effects of caffeic acid phenetyl ester (CAPE) on these changes. For this purpose, 21 male Wistar rats were divi-ded into three groups. Animals in Group I were used as controi. Rats in Group II were exposed to cigarette smo¬ke and rats in Group ili were exposed to cigarette smoke and injected daily with CAPE. At the end of the 60-days experimental period, ali rats were killed by decapitation. Serum testosterone analysis were perfonmed in the blo-od samples obtained from the animals. Follovving routine histological procedures, testicular tissue specimens u/e¬re examined under a light microscope. İn our study, serum testosterone levels and diameters of seminiferous tu-bules were found to be decreased in rats exposed to cigarette smoke and these were prevented by the administ-ration of CAPE. Light microscopic examination of testis specimens from rats exposed to cigarette smoke revealed that congestion of vessels and increase of connective tissue in the interstitial space. Additionally, there was vacuoiî-zation İn the seminiferous tubules anda decrease in spemnatogenetic celis. Whereas, exposure of cigarette smoke-induced histological changes were not seen in rats exposed to cigarette smoke and injected with CAPE. İn conc-lusion, it was observed that testicular functions of rats exposed to cigarette smoke were decreased and this dec¬rease was prevented by administration of CAPE
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Abstract (Original Language): 
Bu çalışmada, sigara dumanına maruz kalan sıçanlara ait teslislerde meydana gelen histolojik değişikliklerin ince¬lenmesi ve bu değişiklikler üzerine kafeik asit fenetil ester (CAPE)'in etkisinin araştırılması amaçlandı. Bu amaçla 21 adet Wistar cinsi erkek sıçan üç gruba ayrıldı. Grup l'deki hayvanlar kontrol grubu olarak kullanıldı. Grup ll'de-ki sıçanlar sigara dumanına maruz bırakıldı. Grup ill'deki sıçanlara ise sigara dumanına maruziyet ile birlikte gün¬lük olarak CAPE enjekte edildi. 60 günlük deney süresi sonunda tüm sıçanlar dekapitasyonla öldürüldü. Hayvan¬lardan alınan kanların serumlarında testosteron analizi yapıldı. Testis doku örnekleri rutin histolojik prosedürlerden geçirilerek ışık mikroskobunda incelendi. Çalışmamızda, sigara dumanına maruz kalan sıçanların serum testoste¬ron seviyeleri ve seminifer tubul çaplarında bir düşüşün olduğu ve bu düşüşün CAPE uygulaması ile engellendiği tespit edildi. Sigara dumanına maruz bırakılan sıçanlara ait testislehn ışık mikroskobik incelenmesinde ise, inters-tisyel alanlardaki damarlarda konjesyonun olduğu ve bağ dokusunun arttığı gözlendi. Ayrıca, seminifer tubullerde vakuoiizasyonun olduğu ve spermatogenik hücrelerin azaldığı görüldü. Sigara maruziyeti ile birlikte CAPE uygula¬nan sıçanlarda ise, sigara maruziyetinin neden olduğu histolojik değişikliklerin oluşmadığı tespit edildi. Sonuç ola¬rak, sigara dumanına maruz kalan sıçanların testis fonksiyonlarında azalmanın olduğu ve bu azalmanın CAPE uy¬gulaması ile Önlendiği görüldü.
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