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Mesanenin değişici epitel hücreli karsinomlannda AgNOR ve mitotik indeks yöntemlerinin tümör grade'i ile ilişkisi

To compare of AgNOR and MI methods in transiüonal celi carcinoma of urinary bladder and to study correiation between tumor grade

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
This study was designed to compare AgNOR and MI methods which are known as proliferation indexes in transitionai celi carcinoma of urinary bladder (TCCJ and to study correiation and regression between grade and AgNOR and MI methods. At the same time we aimed to identify any threshold valve which could help determıne grade and prog-nosis. İn this study, 102 cases ofTCC were involved which were diagnosed between 2000 and 2003 in the Pathology Department of Meram Medical Faculty, Selçuk University. As control group, thirthy eystitis cases vvhose epitheiia were normal in the surface apperance were chosen. Microscopic features of the cases were retrospectively re-evaluated in the iight of latest literatüre. To grade TCC cases we chose the system proposed by Ash. Cases were re-evaluated ınde-pendently of their previous diagnoses. Stainıng and counting systems proposed for AgNOR by Crocker were used vvhen counting AgNOR spots, spots stained black with si/ver in the nucleolus (AgNOR 1} and those both in nucleus and nucleolus (AgNOR llj vvere counted, for each case, in 100 celis vvithout noticing size and distribution. When per-forming Mi method proposed by Simpson. we calculated possible celi and mitosis counts in ten different microscop¬ic areas. The number obtained by a ratio of total mitosis count to possible celi count was accepted as Mi and denom-inator was expressed as 1000. We have found thatAgNOR and Mi values inereased associating grade of the tumor. We could demonstrate a correiation between MI and grade though there was not such a correiation.
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Abstract (Original Language): 
Bu çalışma mesanenin Değişici Epitel Hücreli Karsinom (DEHKj tanısı alan olgularında proliferatif belirleyiciler olan AgNOR ve Mitotik İndeks (MI) yöntemlerini karşılaştırmayı, grade ile korelasyon ve regresyonunu incelemeyi, grade'in tespitinde ve prognoz hakkında kesin yorumda bulunmayı mümkün kılacak bir eşik değerin bulunup bulunamaya¬cağını araştırmak amacı ile planlandı. Bu amaçla Selçuk Üniversitesi Meram Tıp Fakültesi Patoloji Anabilim Dalı'nda 01 Ocak 2000 ve 31 Ocak 2003 tarihleri arasında mesanenin DEHK tanısı alan 102 olgu çalışmada kullanıldı. Ayrıca kont¬rol grubu amacıyla, yüzey epiteli normal görünümdeki, sistit tanısı almış olan 30 olgu çalışmaya dahil edildi. DEHK tanısı almış olguların yeniden grade'lenmesî için Ash'ın önerdiği sistem esas alındı ve eski tanılarından bağımsız olarak yeniden değerlendirildi. AgNOR yöntemi için Crocker'ın önerdiği boyama ve sayım sistemi kullanıldı. AgNOR benek¬lerini sayarken 100 hücrede gümüş ile siyah boyanmış nükleolus içindeki AgNOR benekleri (AgNOR ij ile nükleolus içi + nükleus içi AgNOR benekleri (AgNOR 11} sayıldı. Simpson'un önerdiği MI yöntemini uygularken MI: mitoz/ tahmini hücre sayısı sonucu elde edilen değer payda 1000 olacak şekilde ifade edildi. Mesanenin DEHK'larında grade arttıkça ortalama AgNOR ve MI değerlerinin arttığı, fakat Mi ile arasında anlamlı bir ilişki bulunmadığı görüldü.
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