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Sepsiste indüklenebilen nitrik oksit sentaz inhibitörü (İNOS) ve antioksidanların barsak hasarına etkileri

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
Objective: Nitric oxide (NO) and free oxygen radicals have been implicated in the pathogenesis of intestinal damage and intestinal circulatory dysfunction İn sepsis. The aim of this study is to investigate the effects of pentoxyfıllİn, L-arginin and Aminoguanİdine on plasma malondialdehide (MDA) and nitric oxide (NO) levels, and to determine the relations betvveen MDA and NO levels on intestine pathology. Methods: 60 Wistar Albino rats vvere used in this study. The rats were divided into 6 groups, each containing 10 subjects. Sepsis was induced by cecai ligation and puncture (ÇLP) method. Group I: Sham group, Group II: CLP (sepsis), Group III: CLP + 10 mg/kg L-arginin administration, Group İV: CLP + 15 mg/kg Aminoguanİdine administration, Group V: CLP + L-arginin + Aminoguanİdine (as groups İli and IV) Group VI: CLP+15mg/kg/day pentoxyfillin. Blood samples vvere taken for the determination of NO, MDA leukocyte counts, a segment of ileum tissue sample was obtained for determination oftissue damage. Results: Leukocyte count increased in CLP induced groups signifıcantly. While NO levels vvere significantly higher in sepsis and L-arginin groups, the levels in Aminoguanİdine and Aminoguanİdine + L-arginin groups vvere similar to Sham group. İntestine tissue damage in sepsis and L-arginin groups vvere more severe than the other groups. MDA levels in CLP induced groups were found to be higher than the sham group. Conclusion: Aminoguanİdine and Pentoxifıllin may be added to treat-ment protocols in sepsis in order to prevent intestinal tissue damage and dysfunction both of vvhich (at least par-tially) caused by elevated NO levels.
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Abstract (Original Language): 
Amaç:Deneysel sepsis modelinde pentoksifılin, L-arginin ve aminoguanidin'in plazma nitrik oksid (NO) ve malondi-aldehit (MDAj düzeylerine etkisini belirlemek NO ve MDA düzeyleri ile barsak hasarı arasındaki ilişkiyi ve nitrik oksit inhibitörü aminoguanidin ile tedavinin etkilerini incelemektir. Sepsiste barsakiarda görülen doku hasarının nedeni bak¬teriye! transiokasyon, İntestinal iskemidir. Bu hasar ile NO ve serbest oksijen radikallerinin ilişkisi ve aminoguanidin ile tedavinin etkileri araştırılmıştır. Yöntem: Çalışmada Wistar albino cinsi 60 rat kullanıldı. Ratlar 10'arlı 6 gruba ayrıldı. Ratlarda çekum ligasyon-perforasyon (ÇLP) yöntemi ite sepsis geliştirildi. Ratlar, Grup I: Sham işlemi. Grup II: ÇLP (sep¬sis). Grup III: ÇLP + 10 mg/kg L-arginin, Grup IV: ÇLP + 15 mg/kg Aminoguanidin , Grup V: ÇLP + L-argınin 4 Aminoguanidin (aynı dozlar). Grup VI: ÇLP+ ISmg/kg Pentoksifılin şeklinde gruplara ayrıldı. İşlemden 24 saat sonra ratlardan NO, MDA lökosit tayini için kan örnekleri ve doku hasarını belirlemek için ileum örnekleri alındı. Bulgular: Lökosit sayısı ÇLP uygulanan grup/arda anlamlı olarak arttı. NO düzeyleri sepsis grubu ve L-arginin grubunda anlamlı olarak yükselirken, Aminoguanidin ve Aminoguanidin+L-arginin gruplarında sham grubuna benzer bulundu. ÇLP uygulanan gruplarda barsak doku hasarı, sham grubuna göre anlamlı olarak yüksekti, ancak Pentoksifılin, Aminoguanidin ve Aminoguanidin+L-arginin gruplarının değerleri sepsis ve L-arginin gruplarına göre anlamlı olarak düzelme gösterdiler. Sepsis ve L-arginin gruplarındaki MDA düzeyleri sham grubuna göre anlamlı olarak yüksek bulun¬du. Sonuç: Sepsiste oluşan barsak doku hasarına artan MDA ve NO düzeylerinin neden olduğunu ve bu hasan önle¬mek amacıyla pentoksifılin ve aminoguanidin tedavi protokollerine eklenebileceğini düşünmekteyiz.
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  • 2
74-79
  • Turkish

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