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Deneysel akut kolesistitte inflamasyon ve fibrozise seks hormonlarının etkisi

The effects of sex hormones at inflammation and fibrosis of experimental acut cholecystitis

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2006

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
Aim: To identify the effects of sex hormones on inflammation and fibrosis on and around gall bladder. Material and method: In this study 48 rabbit [24 male, 24 female] was used. Acute cholecystitis was achived by cystic canal ligature [SCL) except control groups. Female (DI,DI!,D!II,DIV) and male [LI,LII,LIII,L:IVj groups had 4 sub¬groups, each consist of 6 members. Macroscopic findings of acute cholecystitis were evoluated. Adhesive bands around gall bladder and biopsies from gall bladder were protected in deep freezer. Fibrotic bands and gall bladder were analyzed for hidroxyprolin (HP) and collagen. Results: Acute cholecystitis was developed in all subjects macroscópica I ly. On male subjects adhesions around gall bladder were seen more intensively. Result of subjects with ooferectomy were close to male subjects. On castrated male subjects adhesions were similar to female subjects. The adhesşons were less at female subjects with ooferectomy with external estrogen use. The lesions were more at castrated male subjects with external testosteron use. These findings were relevant with quantitative values of HP. Collagen were found more in male subjects compored to female subjects. Conclusion: The results is castrated males were similar to those in females and with usage of external testosteron male type results were achieved. And the similarity of results in female subjects with ooferectomy to males achivement of female type results with usage of external estrogens clearly shows us inflammation and fibrosis increasing effect of male sex hormones in acute cholecystitis.
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Abstract (Original Language): 
Amaç: Deneysel akut kolesistitte safra kesesi çevresinde ve duvarında inflamasyon ve fibrozise seks hormonlarının etkisini araştırmak. Gereç ve yöntem: Çalışmada 48 tavşan [24 erkek ve 24 dişi) kullanıldı. Akut kolesistit kontrol grupları dışındaki gruplarda sistik kanal ligatürü (SKL) ile oluşturuldu. Dişi (DI,DII,DIII,DIV) ve erkek (EI,EII,EIII,EIV) denekler 6'şar denekten oluşan dörder alt grubu ayrıldı. Akut kolesistitin makroskopik bulguları değerlendirildi. Safra kesesi çevresindeki adeziv bandlar ve ardından çıkarılan ssfra kesesi duvarından alınan örnekler derin don¬durucuda korundu. Fibrotik bandlar ve safra kesesi kollagen ve hidroksiprolin (HP) için değerlendirildi. Bulgular: Makroskopik olarak tüm deneklerde akut kolesistit gelişti. Erkeklerde safra kesesi çevresinde daha yoğun yapışıklık görüldü. Ovariektomili deneklerde erkeklere yakın derecede kastre erkeklerde ise dişilere benzer yapışıklık izlen¬di. Ovariektomili dişi deneklere dıştan östrodiol verildiğinde yapışıklıkların kontrol grubuna göre daha az olduğu saptandı. Yine kastre erkeklere dıştan testosteron verildiği zaman yapışıklıkların kontrol grubuna göre daha şiddetli olduğu görüldü. Bu bulgular HP'nin kantitatif değerleriyle de uyumluydu. Safra kesesi duvarında kollajen yoğunluğu erkek deneklerde dişilerden daha yüksek bulundu. Sonuç: Kastre erkek deneklerdeki akut kolesistit sonuçlarının dişilere benzer olması ve dıştan verilen testosteronla tekrar erkeklerdeki bulguların oluşması veya ovariektomili denek sonuçlarının erkeklere benzerliği ve dışarıdan verilen östrojen (östradiol) ile benzerliğin kay¬bolması akut kolesistitlerdeki inflamasyon ve fibrozisi artırıcı yönde erkek seks hormonlarının etkisini açıkça göster¬mektedir.
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  • Turkish

REFERENCES

References: 

1. Capizzi FD, Fogii L, Brufatti M, Boschi S. Di Domenico M, Papa V, Patrizi P. Conversion rate in laparoscopic chole-cystectomy: evolution from 1993 and current state. J Laparoendosc Adv Surg Tech A 2003; 13: 89-91.
2. Rosen M, Brody F, PonskyJ. Predictive factors for conver¬sion of laparoscopic cholecystectomy. Am J Surg 2002; ¡84: 254-8.
3. Wiebke EA, Pruitt AL. Howard TJ, Jacobson IE, Broadie TA, Goulet RJ if. Canal "DF. Conversion of laparoscopic to open cholecystectomy. An analysis of risk factors. Surg Endosc 1996; 10: 742-5.
4. Cox MR, Wilson TG, LuckAJ, Jeans PL. Padbury RT, Toouli J. Laparoscopic cholecystectomy for acute inflammation of the gallbladder. Ann Surg 1 993; 2 i 8: 630-4.
5. Shea JA, Healey MJ, Berlin JA, Clarke JR, Malet PF, Staroscik RN, Schwartz J S, Williams SV. Mortality and com¬plications associated with laparoscopic cholecystectomy. A meta-analysis. Ann Surg 1996;224:609-20.
6. Nair RG, Dunn DC, Fowler S, McCloy PF. Progress with cholecystectomy: improving results in England and Wales. Br J Surg 1997; 84: 1396-8.
7. Sanabria JR, Gallinger S, Croxford R, Strasberg SM. Risk fac¬tors in elective laparoscopic cholecystectomy for conver¬sion to open cholecystectomy. J Am Coll Surg 1 994; 1 79: 696-704.
8. Peters JH, krailadsiri W, Incarbone R, Bremner CG, Froes E, Ireland AP. Crookes P. Ortega AE, Anthone GA, Stain SA. Reasons for conversion from laparoscopic to open cholecystectomy in an urban teaching hospital. Am J Surg 1994; 168: 555-9.
9. Kanaan SA Murayama KM, Merriam LT, Dawes LG, Prys-towsky JB, Rege RV, Joehl RJ. Risk factors for laparos-copic to open cholecystectomy. J Surg Res 2002; 106: 20-4.
10. Lein HH, Huang CS. Male gender: risk factor for severe symptomatic cholelithiasis. World J Surg 2002; 26: 598-601.
1 1. Russeil JC, Walsh SJ, Reed-Fourquet L, Mattie A, Lynch J, Symptomatic cholelithiasis: a different disease in men? Connecticut Laparoscopic Cholecystectomy Registry. Ann Surg 1998; 227: 195-200.
1 2. Liu CL, Fan ST, Lai EC, Lo CM. Chu KM. Factors affecting conversion of laparoscopic cholecystectomy to open sur¬gery. Arch Surg 1996; 131:98-101.
13.Alponat A, Kum CK. Koh BC Rajnakova A, Goh PM. Predictive factors for conversion of laparosopic choleys-tectomy. World J Surg 1997; 21: 629-33.
14. Trondsen E, Rosseland AR, Clausen AR, Bakka A, Engh E, Rodland EA, Aasen AO. An experimental study on effects of occlusion of the cystic duct in mice with or without emptying of the gallbladder. Eur Surg. Res. 1997; 29(2[: 150-8
15. Granat M, Tur-Kaspa I, Zylber-Katz E, Schenker JG. Reduction of peritoneal adhesion formation by colchicine: a comparative study in the rat. Fertil Steril 1 983; 40: 369¬72.
1 6. Graham MF, Willey A Adams J, Yager D. Diegelmann RF. Role of ascorbic acid in procollagen expression and secre¬tion by human intestinal smooth muscle cells. J Cell Physiol 1995; 162: 225-33.
1 7. Kama IMA Kologlu M, Doganay M, Reis E, Atli M, Dolapçı M. A risk score for conversion from laparoscopic to open cholecystectomy. Am J Surg 2001; 181: 520-5.
1 8. Woess JF Jr. The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch Biochem Biophys 1 961 May; 93: 440-7
19. Zisman A, Gold-Deutch R, Zisman E, Negri M, Halpern Z, Lin G, Halevy A.. is male a risk factor for conversion of laparoscopic into open cholecystectomy? Surg Endosc 1996; 10: 892-4.
20. Frazier-Jessen MR, Mott FJ, Witte PL, Kovacs EJ. Estrogen suppression of connective tissue deposition in a murine model of peritoneal adhesion formation. J Immunol 1996; 156: 3036-42.
21. Kartal A, Aksoy F, Vatansev C, Şahin M, Yilmaz O, Belvlranli M, Karahan O. Does estrogen cause low conversion rates in laparoscopic cholecystectomies for acute and chronic cholecystitis in women? JSLS 2001; 5: 309-12.
22. Shamberger RC, Thistlethwaite PA, Thibault LE, Talbot TL, Brennan MF. The effect of testosterone propionate on wound healing in normal and castrate rats. J Surg Res 1982; 33: 58-68.
23. Wichmann MW, Ayala A, Chaudry IH. Male sex steroids are responsible for depressing macrophage immune func¬tion after trauma-hemorrhage. Am J Physiol 1997; 273: 1335-40.
24. Frazier-Jessen MR, Kovacs EJ. Abdominal wall thickness as a means of assessing peritoneal fibrosis in mice. J Immunol Methods 1993; 162: 115-21.
25. Kartal A, Vatansev C, Aksoy F. Boz S, Aktan M, Gölcük M. Inflammation and fibrosis of cholecystitis in males is more than in females. 5th European Congress of the IHPBA May 28-31, 2003 Istanbul, Turkey, HPB 2003;5 supplement 1; 54.

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