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HEPARİN'İN İN VİTRO ORTAMDA DAMAR DÜZ KAS HÜCRE MİTOZUNA İNHİBİTÖR ETKİSİ

The inhibitory effect of heparine on mitosis of in vitro smooth muscle cell

Journal Name:

  • Selçuk Tıp Dergisi

Publication Year:

  • 2007

Key Words:

Keywords (Original Language):

Author NameUniversity of Author
Abstract (2. Language): 
Aim: Heparine is used during some clinical situations. We aimed to reveal heparin's effect on vessel smooth muscle mitosis under in vitro conditions. Material and Method: Smooth muscle cells we¬re obtained from the 4th passage of cryostored cell suspension prepared from rabbit femoral artery in cell culture laboratory. The 5th passage was studied as control and study group for four days by adding heparine to the medium. Final cell number was calculated and statistical comparison was done. Results: In the 5th passage smooth muscle cells, of control (G1) and heparin applied (G2) groups, heparin caused a statistically significant (p<0,05) inhibitory effect on cell mitosis. Conclusion: Heparin inhibits mitosis of vascular smooth muscle cells.
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Abstract (Original Language): 
Amaç: Heparin bazı klinik durumlarda kullanılmaktadır. Heparin'in in vitro ortamda damar düz kas mitozuna etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Hücre kültür laboratuarında tav¬şan femoral arterinden hazırlanan damar düz kaslarının cryobank'ından (4.pasaj) çözülen hücreler kültüre alındı. 5. pasajda heparin ve sham grubu olmak üzere 4 gün uygulandı ve süre sonunda top¬lam hücre sayıları belirlendi. istatistiksel karşılaştırma yapıldı. Bulgular: Kontrol (G1) ve heparin uygu-lanan (G2) düz kas hücrelerinin 5. pasaj sonunda elde edilen hücre sayılarında heparinin hücre mitozu üzerinde istatistiksel olarak anlamlı (p<0,05) inhibitör etkiye neden olduğu görüldü. Sonuç: Heparin damar düz kas hücrelerinde mitozu inhibe etmektedir..
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  • 1
7-10
  • Turkish

REFERENCES

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