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Candida bombicola ATTC 22214 MAYASI İLE GIDA SEKTÖRÜNE YÖNELİK BİYOSURFAKTANT ÜRETİMİ

PRODUCTION OF SOPHOROLIPIDS USING THE YEAST Candida bombicola ATTC 22214 FOR THE APPLICATIONS IN THE FOOD INDUSTRY

Journal Name:

  • Sigma Mühendislik ve Fen Bilimleri Dergisi

Publication Year:

  • 2006

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Lactonic sophorolipids were produced by the yeast Candida Bombicola ATTC 22214 (Torulopsis bombicola) in a 3L bioreactor using batch cultivation, with corn oil and glucose as carbon sources. The main compound of the lactonic mixture was separated from the mixture by column chromotography and was identified as 17 hydroxyoctadecanoic-1’,4’’-lactone 6’ 6’’ diacetate sophorolipid. Surface tension measurements showed that both the crude lactonic mixture and the diacetyl lactone lowered the surface tension of pure water from 72 din cm-1 to 43,5 din cm-1 and 38.9 din cm-1 respectively thus proving that both samples were surface active. The bio-product consists of only sophorose sugar and lipid in its structure and this can safely be used in food industry as a biosurfactant, either in its crude mixture form or in purified diacetylactonic form.
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Abstract (Original Language): 
Karbon kaynağı olarak mısır özü yağı ve glukoz içeren besi ortamında, 3L biyoreaktörde ve kesikli yöntemle Candida Bombicola ATTC 22214 (Torulopsis bombicola) mayası kullanarak laktonik soforozlipidleri üretilmiştir. Soforozlipidleri karışımındaki temel bileşik, kolon kromotografisi yöntemiyle karışımdan ayrıştırılmış ve bileşiğin 17 hidroksioktadekanoik-1’,4’’-lakton 6’ 6’’ diasetat soforozlipid olarak NMR tanısı yapılmıştır. Laktonik karışım ve ayrıştırılan diasetillenmiş lakton, saf suyun ortalama 72 din cm-1 olan yüzey gerilimini sırasıyla 43,5 din cm-1 ve 38.9 din cm-1 değerlerine düşürmüş ve böylece her iki ürünün de yüzey aktif özellikleri irdelenmiştir. Biyo-ürün, yapısında yalnızca soforoz şekeri ve yağ içerdiği için gerek saflaştırılıp temel bileşenine ayrılarak gerekse de saflaştırılmadan gıda sektörüne yönelik uygulamalarda biyosurfaktant olarak güvenle kullanılabilecektir.
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  • 2
109-116
  • Turkish

REFERENCES

References: 

[1] Vardar-Sukan F., Kozaric N., Biosurfactants in Encyclopedia of Microbiology ( Ed:
J.Lederberg) Vol.1, 2nd ed, Academic Press, San Diego 2000, 618-635.
[2] Deleu M.,Paquot M., “From renewable vegetable resources to micro organisims: New
trends in surfactants”, Chimie, 7, 641-646, 2004.
[3] Banat I.M., Makkar R.S., Cameotra S.S., “Potential commercial applications of microbial
surfactants”, Appl.Microbiol. Biotechnol., 53, 495-508, 2002.
[4] Asmer H.J., Lang S., Wagner F., Wray V., “Microbial production, structure elucidation
and bioconversition of sophorose lipids”, J. Am. Oil Chem.Soc., 65,1460-1466,1998.
[5] Tulloch A.P., Hill A., Spencer J.F.T., “Structure and reaction of lactonic and acidic
sophorosides of 17-dioxyoctadecanoic acid”, Can. J. Chem., 46, 3337-3351, 1968.
[6] Weber L., Stach J., Haufe G., Hommel R., Kleber H.P., “Elucidation of structure of an
unusual cyclic glycolipid from Torulopsis apicola”, Carbohyr. Res., 206 (1), 13-19,1990.
[7] Otto R.T., Daniel H.J., Pekin G., Müller-Decker K., Fürstenberger G., Reuss M., Syldatk
C., “Production of sophorolipids from whey II. Product composition,surface active
properties,cytotoxicity and stability against hydrolases by enzymatic treatent”, Appl.
Microbiol. Biotechnol., 52, 495-501, 1999.
[8] Davila A.M., Marchal R., Vanecasteele J.P., “Kinetics and balance of a fermentation free
from product inhibition: sophorose lipid production by Candida bombicola”, Appl.
Microbiol. Biotechnol., 38, 6-11, 1992.
[9] Tulloch A.P,, Spencer J.F.T., Gorin P.A.J., “The fermentation of long chain compounds
by Torulopsis magnoliae I. Structures of the hydroxyl fatty acids obtained by the
fermentation of fatty acids and hydrocarbons”, Can. J. Chem., 40, 1326-1338, 1962.
[10] Rau U., Hammen S., Heckman R., Wray V., Lang S., “Sophorolipids: A source of novel
compounds”, Ind. Crop. Prod., 13 (2), 85-92, 2001.
[11] Daniel H. J., Reuss M., Syldatk C., “Production of sophorolipids in high concentration
from deproteinized whey and rapeseed oil in two stage fed-batch process using Candida
Bombicola ATTC 22214 and Cryptococcus curvatus ATCC 20509”, Biotechnol. Lett.,
20, 1153-1156, 1998.
[12] Klekner V., Kosaric N., Zhou Q.H., “Sophorose lipids produced from sucrose”,
Biotechnol. Lett., 13, 345-348, 1991.
[13] Zhou Q.H., Klekner V., Kosaric N., “ Production of sophorose lipids by Torulopsis
bombicola from sunflower oil and glucose”, J. Oil Chem. Soc., 69, 89-91,1992.
[14] Jones D.F., Howe R., “Microbiological oxidation of long-chain aliphatic compounds, Part
I, Alkanes and alk-1-enes”, J. Chem. Soc., 2801-2808, 1968.
[15] Deshande M., Daniels L., “ Evaluation of sophorolipid biosurfactant production by
Candida Bombicola using animal fat”, Biores. Technol., 54, 143-150, 1995.
[16] Bednarski W., Adamcz M., Tomasik J., Plaszczyk M., “ Application of oil waste in the
biosynthesis of glycolipids by yeast”, Biores. Technol., 95, 15-18, 2004.
[17] Brakemeier A., Wullbrant D., Merschel L., Bennighoven A., Buschmann N., “Novel
sophorose lipids from microbial conversion of 2-alkanols”, Biotecho. Lett., 17, 1183-
1188, 1995.
[18] Rau U., Manzke C., Wagner F., “Influence of substrate supply on the production of
sophorose lipids by Candida Bombicola ATTC 22214”, Biotechnol. Lett., 18, 149-154,
1996.
[19] Daniel H.J., Otto R.T., Reuss M., Syldatk C., “ Production of sophorolipids from whey:
Development of two stage fed-batch process with Cryptococcus curvatus ATCC 20509
and Candida Bombicola ATTC 22214 using deproteinized whey concentrates as
substrates”, Appl. Microbiol. Biotechnol., 51, 40-45, 1999.
[20] Pekin G., Vardar-Sukan F., Kosaric N., “Production of sophorolipids from Candida
Bombicola ATTC 22214 using Turkish corn oil and honey”, Eng. Lif. Sci., 5, 4, 357-362,
2005.
[21] Davila A.M., Marchal R., Vandecasteele J.P., “Sophorose lipid production from lipidic
precursors: Predictive evaluation of industrial substrates ”, J. Ind. Mcrobiol., 13,249-257,
1994.
[22] Koster C.G., Heerma W., Peapermans H.A.M., Groenewegen A., Peters H., Haverkamp
J., “Tandem mass spectrometry and nuclear magnetic resonance spectroscopy studied of
Candida bombicola sophoroipids and product formed on hydrolysis by cutinase”, Anal.
Biochem., 230, 135-138, 1995.

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