Buradasınız

“HORSERADISH” PEROKSİDAZ-DEKSTRAN KONJUGATININ “NAPHTOL BLUE BLACK” ÜZERİNE ETKİSİ

HE EFFECT OF HORSERADISH PEROXIDASE (HRP)-DEXTRAN CONJUGATE ON NAPHTOL BLUE BLACK

Journal Name:

Publication Year:

Abstract (2. Language): 
The removal of azo dye Naphtol Blue Black in aqueous media with the catalysis of HRP-dextran conjugate was studied. HRP was purified by affinity chromatography using immobilized Con A. Enzyme-dextran conjugate with 1/10 molar ratio was synthesized using dextran aldehyde derivative (Dextran Mw 75.000 Da) and purified enzyme. The reaction was carried out at pH 3, 4, 5, 6, 7 and 8 in a 25, 30, 35, 40, 45, 50, 60, 70 o C temperatured crystal cuvette of UV-Vis spectrophotometer. The bleaching of the dye depending on the time was observed with the absorption spectra recorded regularly for one hour. The best results were obtained at pH 4 and 5 for all temperatures studied.
Abstract (Original Language): 
Bu çalışmada azo boyar madde “Naphtol Blue Black” ’in sulu ortamda HRP-Dekstran konjugatı katalizi ile giderilmesi incelendi. HRP immobilize Con A kullanılarak affinite kromatografi yöntemi ile saflaştırıldı. Dekstran aldehid türevi (Dekstran Mw 75.000 Da) ve saf enzim kullanılarak 1/10 oranında enzim-dekstran konjugatı sentezlendi. Reaksiyon pH 3, 4, 5, 6, 7 ve 8’de UV-Vis. spektrofotometrenin 25, 30, 35, 40, 45, 50, 60, 70 o C sıcaklığa ayarlanmış kristal küvetinde gerçekleştirildi. Zamana bağlı olarak boyada renk giderme düzenli bir şekilde alınan absorpsiyon spektrumları ile bir saat süreyle izlendi. En iyi sonuçlar çalışılan bütün sıcaklıklar için pH 4 ve 5’de elde edildi.
FULL TEXT (PDF): 
18-25

REFERENCES

References: 

[1] Klibanov, A. M., Stabilization of enzymes against thermal inactivation, Adv. Appl.
Microbiol., 29, 1-28, 1983.
[2] Dec J, Bollag J-M., Use of plant material for the decontamination of water polluted with
phenols, Biotechnol Bioeng 44:1132–1139, 1994.
[3] Bhunia, A., Durani, S., Wangikar, P.P., Horseradish Peroxidase Catalyzed Degradation of
Industrially Important Dyes, Bıotechnology And Bıoengıneerıng, 72:5, 562-567, 2001.
[4] Liu, J.Z., Wang, T.L., Ji, T.L., Enhanced dye decolorization efficiency by citraconic
anhydride-modified horseradish peroxidase, Journal of Molecular Catalysis B: Enzymatic,
41, 81–86, 2006.
[5] Franco Fragus, L., Batista-Viera F., Carlsson J., Preparation of high-density Concavalin A
adsorbent and its use for rapid high-yield purification of peroxidase from horseradish
roots, J. Chromatogr. B., 803, 237-241, 2004.
[6] Leon, J.C., Alpeeva, I.S., Chubar, T.A., v.d., Purification and Substrate Specificity of
Peroxidase from Sweet Potato Tubers, Plant Science, 163, 1011-1019, 2002.
[7] Desphande, S.S., Enzyme Immunoassays, chapter 5, Chapman & Hall, New York, 1996.
[8] Carvalho, A.S.L., Neves-Petersen, M.T., Peterson, S.B., v.d., Formation of a misfolded
conformation during refolding of HRPA1 in the presence of calcium, Biochim. Et Biophy.
Acta, 1747, 99-107, 2005.
[9] Marshall, J.J., Manipulation of the Properties of Enzymes by Covalent Attachment of
Carbohydrate, TIBS, 3, 79-83, 1978.
[10] Betancor, L., Gallego, F., L., Hidalgo, A., v.d., Prevention of interfacial inactivation of
enzymes by coating the enzyme surface with dextran-aldehyde, Journal of Biotechnology,
110, 201-207, 2004.
[11] Abian, O., Wilson, C., Fernandez-Lorente, G., v.d., Preparation of Artifical Hyperhydrophilic Micro-Environments (Polymeric salts) Surrounding Enzyme Molecules New
Enzyme Derivatives to be Used in any Reaction Medium, Journal of Molecular Catalysis
B: Enzymatic, 19-20, 295-303, 2002.
[12] Al-Momani, F., Touraud, E., Degorce-Dumas, J.R., v.d., Biodegradability enhancement of
textile dyes and textile wastewater by VUV photolysis, Journal of Photochemistry and
Photobiology, 153, 191-197, 2002.
[13] Stolz, A., Basic and applied aspects in the microbial degradation of azo dyes, Applied
Microbiology and Biotechnology, 56, 69-80, 2001.
[14] Borker, P., Salker, A. V., Solar assisted photocatalytic degradation of Naphthol Blue
Black dye using Ce1−x
Mnx
O2, Materials chemistry and physics, 103, 336-370, 2007.
[15] Troupis, A., Gkika, E., Triantis, T., vd., Photocatalytic reductive destruction of azo dyes
by polyoxometallates: Naphthol blue black, Journal of photochemistry and photobiology,
188, 272-278, 2007.
[16] Chunjie, J., Yihang, G., Changwen, H., vd., Photocatalytic degradation of dye naphthol
blue black in the presence of zirconia-supported Ti-substituted Keggin-type
polyoxometalates, Materials Research Bulletin, 39, 251-261, 2004.
[17] Jin, L., Maria, H., Photoelectrochemical degradation of naphthol blue black diazo dye on
WO3
film electrode, Electrochimica Acta, 46, 2913-2922, 2001.
[18] Gültekin, I., Ince, N.H., Degradation of aryl-azo-naphthol dyes by ultrasound, ozone and
their combination: Effect of α-substituents, Ultrasonics Sonochemistry, 13, 208-214,
2006.
[19] Bring, H. B., Dekker, H. L., Schoemaker, H. E., v.d., Oxidation reactions catalyzed by
vanadium chloroperoxidase from Curvularia inaequalis, Journal of Inorganic
Biochemistry, 80, 91–98, 2000.
[20] Altıkatoğlu, M., HRP-Dextran Konjugatları, Doktora tezi, Fen-Bilimleri Enstitüsü, Y.T.Ü,
2007.
[21] Sacco, D., Bonneaux, F., Dellacherie, E., Interaction of haemoglobin with dextran
sulphates and the oxygen-binding properties of the covalent conjugates, J. Biol.
Macromol., 10, 305-310, 1988.

Thank you for copying data from http://www.arastirmax.com