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Stafilokokların fosfataz aktivitesinin bir sentetik hidroksiapatit biomateryalin çözünmesine etkisinin incelenmesi

Investigation of the influence of phosphatase activity of staphylococcus on the dissolution of a synthetic hydroxyapatite biomaterial

Journal Name:

  • Süleyman Demirel Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi

Publication Year:

  • 2010

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Objective: Hydroxyapatite (HA) is widely used as bone graft and as coating for orthopaedic and dental implants. Staphylococci are accepted as the main pathogens of biomaterial-related infections. The aim of this study was to investigate whether the phosphatase activity of various strains of S. epidermidis had an effect on the dissolution of a synthetic hydroxyapatite biomaterial. Method: Ceramic discs were prepared by compressing pure hydroxyapatite powder and sintering at 1200C. The ability of various strains of staphylococci to produce the phosphatase enzyme was evaluated with two different methods. HA discs were incubated with staphylococcal strains of known phosphatase activity and the calcium concentrations of the medium were measured. HA disc surfaces were examined by means of SEM. Results: The results of the Ca concentration measurements of the medium in which HA discs were incubated showed that in the presence of four S. epidermidis with known phosphatase activity, no dissolution occurred from the HA discs. Scanning electron microscope (SEM) analysis revealed that the strains of S. epidermidis tested may adhere to HA discs however no visual signs of dissolution appeared on the surfaces. No correlation was observed between the phosphatase activity of S. epidermidis strains and their adherence to HA surface. Discussion: Phosphatase activity of the strains of S. epidirmidis did not have any influence on the dissolution of hydroxyapatite material tested. For future work, it would be beneficial to do similar experiments on HA plasma-sprayed coating samples.
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Abstract (Original Language): 
Amaç: Hidroksiapatit (HA) kemik grefti olarak ve ortopedik ile dental implantlarda yüzey kaplaması şeklinde yaygın olarak kullanılan bir materyaldir. Stafilokoklar biomateryallere bağlı enfeksiyonlarda esas patojen olarak kabul edilirler. Bu çalışmanın amacı S. epidermidis’in farklı suşlarının fosfataz aktivitelerinin, bir sentetik hidroksiapatit biomateryalin çözünmesinde etkisi olup olmadığını bulmaktır. Yöntem: Seramik diskler saf hidroksiapatit tozunun preslenmesi ve 1200C’de sinterlenmesi ile hazırlandı. Stafilokokların farklı suşlarının fosfataz enzimi üretebilme yetisi iki farklı metotla değerlendirildi. HA diskler fosfataz aktiviteleri bilinen stafilokok suşları ile inkübe edildi ve besiyerindeki kalsiyum konsantrasyonları ölçüldü. HA disk yüzeyleri scanning elektron mikroskobu (SEM) yardımıyla incelendi. Sonuçlar: HA disklerin inkübe edildiği besiyerlerindeki Ca konsantrasyonlarının ölçülmesi, S. epidermidis’in fosfataz aktivitesi bilinen dört farklı suşunun varlığında, HA disklerde çözünme olmadığını gösterdi. SEM ile yapılan analizler, S. epidermidis’in incelenen suşlarının HA disklere tutunabileceğini gösterdi, ancak yüzeylerde gözle fark edilebilir bir çözünmeye rastlanmadı. S. epidermidis suşlarının fosfataz aktivitesi ile HA disklere tutunması arasında bir ilişki gözlenmedi. Tartışma: S. epidermidis suşlarının fosfataz aktivitesinin, incelenen HA materyalin çözünmesi üzerine bir etkisi bulunmamaktadır. İlerideki çalışmalarda HA plazma sprey kaplı örneklerde benzer deneylerin yapılması faydalı olabilir.
FULL TEXT (PDF): 
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  • 2
30-38
  • English

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