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2,4-Diklorofenoksiasetik asit'i n ikinci çapraz yavruların böbreğinde bazı antioxidant enzimlerin aktiviteleri üzerine etkisi

The effect of 2,4-dichlorophenoxyacetic acid on some antioxidant enzymes in kidneys of the second cross offsprings mice

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
2,4-Dichlorophenoxyacetic acid (2,4-D) is a systemic herbicide used to control a wide variety of broad-leaved weeds in agriculture. But, several studies have been suggested that 2,4-D is a carcinogenic, mutagenic, clasto-genic and neurotoxic agent. The aim of the present study was to investigate the effect of 2,4-D on some kidney antioxidant enzymes activities such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) in the second cross offsprings (Mus musculus). Female mice were divided into two equal groups: 2,4-D group and ethanol control group. Two the chemicals used were applied intraperitoneally. 2,4-D was administrated to maternal mice as a 1/100 dilution of 338 mg/kg body weight (LD30) in 3 day intervals. In the control group it was administrated by similar way. Same processes were repeated over two generations. Spectrophotometric method was used to determine the activities of enzymes in the kidney of the second cross offsprings. The activity of CAT was found to be significantly lower in 2,4-D group compared to control ethanol group (p<0.001). There was no significant difference in the SOD and GSH-Px activities between the 2,4-D and control groups. The results obtained suggest that the CAT activity of kidney in the second cross offsprings was affected from 2,4-D toxicity.
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Abstract (Original Language): 
2,4-diklorofenoksiasetik asit (2,4-D) tarımda geniş yapraklı yabani otların kontrolünde kullanılan sistemik bir herbisittir. Fakat, bazı çalışmalar, 2,4-D'in karsinojenik, mutajenik, klastogenik ve nörotoksik bir ajan olduğunu belirtmektedirler. Bu çalışmanın amacı, 2,4-D'in ikinci çapraz yavruların (Mus musculus) böbreğinde antioksidan enzimlerden süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) enzimlerinin aktiviteleri üzerine etkilerini incelemektir. Dişi fareler, 2,4-D ve kontrol gruplarına ayrıldı. 2,4-D anne farelere LD30 dozu olan 338 mg/kg vücut ağırlığı dozundan 1/100 seyreltilerek üç günde bir intraperitoneal(i.p.) olarak verildi. Kontrol grubuna da etanol aynı yolla verildi. Bu işlemler iki nesil boyun¬ca yapıldı. Ikinci çapraz yavruların böbreğinde, enzim aktiviteleri spekrofotometrik olarak ölçüldü. CAT aktivitesi, 2,4-D grubunda, kontrol grubuna göre anlamlı olarak azaldı (p<0.001). SOD ve GSH-Px aktivitelerinde ise anlamlı bir fark gözlenmedi. Sonuç olarak, ikinci çapraz yavruların böbreğindeki CAT aktivitesinin 2,4-D'in toksisitesinden etkilendiği söylenebilir.
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REFERENCES

References: 

1. Turkula TE, Jalal SM. Induced clastogenicity in white rats by the herbicide 2,4-D. Cytologia 1987;52:275-81.
2. Kelley M, Vessey DA. Interaction of 2,4-dichlorophenoxy-acetate (2,4-D) and 2,4,5-trichlorophenoxyacetate (2,4,5-D) with the acyl-CoA: amino acid N-acetyltransferase enzymes of bovine liver mitochondria. Biochem Pharmacol 1986;35:289-95.
3. Ibrahim MA, Bond GG, Burke TA, Cole P, Dost FN, Enterline PE, et al. Weight of the evidence on the human carcinogenicity of 2,4-D. Environ Health Perspect 1991;96:213-22.
4. Rodriguez EM, Schuld M, Romano L. Chronic histopatho-logical effects of parathion and 2,4-D on female gonads of Chasmagnathus granulata (decapoda, brachyura). Fed Chem Toxicol 1994;32:811-8.
5. Palmeira CM, Moreno AJ, Madeira VMC. Interactions of herbicides 2,4-D and dinoseb with liver mitochondrial bioenergetics. Toxicol Appl Pharmacol 1994b;127:50-7.
6. Oakes DJ, Pollak JK. Effects of a herbicide formulation, Tordon 75 D, and its individual components on the oxida-tive functions of mitochondria. Toxicology 1999;136:41-52.
7. Palmeira CM, Moreno AJ, Madeira VMC. Metabolic alter¬ations in hepatocytes promoted by the herbicides paraquat,
dinoseb and 2,4-D. Arch Toxicol 1994a;68:24-31.
8. Pavlica M, Papes D, Nagy B. 2,4-Dichlorophenoxyacetic acidcauses chromatin and chromosome abnormalities in plant cells and mutation in cultured mammalian cells. Mutat
Res 1991;263:77-81
9. Dere E, Yanikoğlu A. The effect of endosulfan and 2,4-D on some enzyme activities on the livers and kidneys of Swiss-Albino (Mus musculus). Doğa-Tr Biology 1993;17:163-173
10. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988;34:479-500.
11. Paglia, D, Valentine, WN. Studies on the quantitive and qualitative charecterization of erythrocyte glutathione per-
8
S.D.Ü. Tıp
Fak
. Derg. 2004;11(2):6-9
Yılmaz,
Th
e effect of 2,4-dichlorophenoxyacetic acid on antioxidant enzymes.
9
oxidase. J Lab Clin Med 1967;70:158-63.
12. Aebi H: In Bergmeter HU(ed). Methods in Enzymatic Analysis, Weinheim, Verlag Chemie 1982;3:273-82.
13. Lowry OH, Rosenbrough NJ, Farr AL, Rondall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193,265-275.
14. Stevens JT, Summer DD. Herbicides. In:Hayes WJ, Laws ER (eds) Handbook of pesticide toxicology, vol 3,
Acedemic Press, New York, 1991;1317-91.
15. Palmeira CM, Moreno AJ, Madeira VMC. Thiols metab¬olism is altered by the herbicides paraquat, dinoseb and 2,4-D: A study in isolated hepatocytes. Toxicol Lett 1995;81:115-23.
16. Yamazaki S, Ozawa N, Hiratsuka A, Watabe T. Photogeneration of 36-Hydroxy-5a-cholest-6-ene-5-hydroperoxide in rat skin: evidence for occurrence of sin¬glet oxygen in vivo. Free Rad Biol Med 1999;27:3/4,301-8.
17. Winzer K, Becker W, Van Noorden CJF, Köhler A. Short-time induction of oxidative stress in hepatocytes of the European flounder (Platichthys flesus) Mar Environ Res
2000;50:495-501.
18. Akyol Ö, Arslanoğlu R, Durak İ. Activities of free radical and DNA turn-over enzymes in cancerous and non-cancer¬ous human brain tissues. Redox Report 1995;1:255-9.
19. Kawashima Y, Katoh H, Nakajima S, Kozuka H, Uchiyama M. Effects of 2,4-dichloropenoxyacetic acid and 2,4,5-trichlorophenoxy acetic acid on peroxisomal enzymes in rat liver. Biochem Parmacol 1984;33:241-5.
20. Bucher, N. Effects of 2,4-dichlorophenoxyacetic acid on experimental animals. Proc Soc Exp Biol 1946;63:204-5.
21. Oruc EO, Uner N. Combined effects of 2,4-D and azin-phosmethyl on antioxidant enzymes and lipid peroxidation in liver of Oreochromis niloticus. Comp Biochem Physiol C Toxicol Pharmacol 2000;127:291-6.
22. Oruc EO, Uner N. Marker enzyme assessment in the liver of cyprinus carpio (L.) exposed to 2,4-D and azinphos-methyl. J Biochem Mol Toxicol 2002;16:182-8.

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