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Melatonin'in intestinal iskemi-reperfüzyonda 6-fosfoglukonat dehidrogenaz aktivitesi üzerine etkisi

The effect of melatonin on 6-phosphogluconate dehydrogenase in intestinal ischemia / reperfusion

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
In this study, enzyme activity of 6-phosphogluconate dehydrogenase (6-PGD) which is one of the key enzyme in the intestinal tissue for intestinal ischemia/reperfusion (I/R) period in pentose phosphate metabolic path¬way was investigated and the effect of melatonin on enzyme activity before and after I/R was determined. A total of 32 young male Sprague Dawley rats were divided into 4 groups (n= 8). Group 1 was placebo and Group 2 was control I/R group. Melatonin (10 mg/kg) and I/R was applied for Group 3 and melatonin (10g/kg) and R was applied for Group 4. I/R was performed by occlusion of superior mezenteric artery for 45 minutes and following reperfusion for 120 minutes. por all groups enzyme specific activity was calculated by dividing volume activity value to total protein amount. The results showed that there is a decrease in the activ¬ity of pentose phosphate metabolic pathway key enzyme 6-phosphogluconate dehydrogenase (6-PGD) for control I/R group during intestinal ischemia- reperfusion period compare to sham group. But the activity val¬ues of the group which melatonin was applied before ischemia was close to the values observed for then sham group. There was no obvious increase in the enzyme activity of the group which melatonin was applied after ischemia as compare to group which melatonin was applied before the ischemia. The differences in all groups were not statistically significant.
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Abstract (Original Language): 
Bu çalışmada, intestinal iskemi-reperfüzyon (I/R) sürecinde, intestinal dokuda pentoz fosfat metabolik yolunun anahtar enzimlerinden biri olan 6-fosfoglukonat dehidrogenaz (6-PGD) enziminin aktivitesi ve melaton-in'in iskemi öncesi ve sonrası uygulanmasının enzim aktivitesi üzerine etkisi araştırıldı. 32 adet Sprague Dawley cinsi erkek sıçan, herbiri 8' er denekten oluşan 4 gruba ayrıldı (n: 8). Grup 1: plasebo, grup 2: I/R (Kontrol), grup 3: 10 mg/kg melatonin+I/R ve grup 4: I+10 mg/kg melatonin + R. I/R uygulaması, superior mezenterik arter (SMA)' in 45 dakika diseke edilmesi ve ardından 120 dakika reperfüzyonu ile gerçekleştiril¬di. Tüm gruplarda enzim spesifik aktiviteleri, volüm aktivite değerleri total protein miktarlarına bölünerek hesaplandı. Elde edilen veriler, intestinal iskemi-reperfüzyon sürecinde 6-PGD aktivitesinde kontrol I/R grubunda plasebo grubuna kıyasla bir azalmanın olduğunu göstermekte, buna karşılık iskemi öncesi mela-tonin uygulanan grupta aktivite değerinin yükselerek plasebo grubuna yakın bir değer aldığı görülmektedir. İskemiyi takiben melatonin verilen grupta ise enzim aktivitesi iskemi öncesi melatonin uygulanan gruptaki kadar bir artış göstermemiştir. Tüm gruplardaki farklılıklar istatistiksel olarak anlamlı değildir.
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  • 3
8-11
  • Turkish

REFERENCES

References: 

1. Cabeza J, Motilva V, Martin MJ, De la Lastra CA. Mechanisms involved in gastric protection of melatonin against oxidant stress by ischemia-reperfusion in rats. Life Sci 2001;68:1405-1415.
2. Slavikova H, Lojek A, Hamar J, Duskova M, Kubala L. Total antioxidant capacity of serum increased in early but not late period after intestinal ischemia in rats. Free Radic Biol Med 1998; 25: 9-18.
3. İşlekel H, İşlekel S, Güner G. Biochemical mechanism and tissue injury of cerabral ischemia and reperfusion. J Neurol Sci 2000; 7:2:1984-2000.
4. Liu TZ, Stern A. Assessment of the role oxidative stress in human diseases. J Biomed Lab Sci 1998;10:12-28.
5. Gözükara EM. Biyokimya Cilt-2, Ankara, Nobel Tıp Kitabevleri. 2000;923-941.
6. Benot S, Goberna R, Reiter RJ, Carcia MS, Osuna C, Guerrero JM. Physiological levels of melatonin contribute to the antioxidant capacity of human serum. J Pineal Res 1999;27:59-64.
7. Rudack D, Gozukara EM, Chisholm ME, Holten, D. The effect of dietary carbohydrate and fat on the synthesis
S.D.Ü. Tıp pak.
Derg
. 2004;11(3):8-11
Ateş,
Melatoni
n 6-fosfoglukonat dehidrogenaz etkisi
11
of rat liver 6-phosphogluconate dehydrogenase. Biochim
Biophys Acta 1971;252:305-313.
8. Lowry O, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurements with the folin phenol reagent. J Biol Chem 1951;193:265-275.
9. Schoenberg MH. Beger HG. Reperfusion injury after intes¬tinal ischemia. Crit Care Med 1993; 21:1376-1386.
10. Augustin AJ, Goldstein RK, Milz JL. Influence of antiinflammatory drugs and free radical scavengers on intestinal ischemia induced oxidative tissue damage. Advances In Experiment Med Biol 1992;316:239-251.
11. Schmeling DJ, Caty MG, Oldham KT, Guice KS. Cytoprotection by diclofenac sodium after intestinal ischemia/reperfusion injury. J Pediat Surg 1994;29:1044-
1048.
12. Koltuksuz U, Ozen S, Uz E, Gültek A. Caffeic acid phenethyl ester prevents intestinal ischemia reperfusion injury in rats. J Ped Surg 1999;34:1458-1462.
13. Hammerman C, Goldschmidt D, Caplan MS, Kaplan M. Amelioration of ischemia-reperfusion injury in rat intestine by pentoxifylline-mediated inhibition of xanthine oxidase. J Pediatr Gasroenterol Nutr 1999;29:69-74.
14. Kazez A, Demirbağ M, Üstündağ B, Özercan İH, Sağlam M. The role of melatonin in prevention of intestinal ischemia-reperfusion injury in rats. J Pediatr Surg 2000;35:1444-1448.
15. Reiter RJ. The pineal and its hormones in the control of reproduction in mammals. Endocr Rev 1980; 1:109-131.
16. Reiter RJ. Melatonin: The chemical expression of dark¬ness. Mol Cell Endocrinol 1991;79:153-158.
17. Schoenberg MH, Beger HG. Reperfusion injury after intestinal ischemia. Crit Care Med 1993;(21):1376-1386.
18. Tan DX, Manchester LC, Reiter RJ, Wen-Bo Q, Malgorzata K, Juan RC. Significance of melatonin in antioxidative defense system: Reactions and products. Biol Signals and Reseptors 2000;(9):137-159.
19. Reiter Russel, J. Oxidative damage in the central nervous system:protective by melatonin. Prog Neurobiol
1998;(56):359-384.

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