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Melatoninin rat akciğer hücreleri ve eritrositlerinde 27.17 mhz radyofrekans radyasyonu ile oluşturulan lipid peroksidasyonu üzerine önleyici etkisi

Melatonin protects the formation of lipid peroxidation induced by 27.17 mhz radiofrequency radiation in rat lung cells and erythrocytes

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
Introduction: A decrease in melatonin secretion has been observed in small mammals under exposure to radio-frequency radiation. As there is some concern about possible health effects of the increasing use of 27.17 MHz citizen band radios emitting radiofrequency electromagnetic fields, we examined whether such fields would alter the level of malondialdehyde (MDA) that is extensively used as a marker of lipid peroxidation. Objective: The purpose of this experiment was to determine whether the exposure of rats to pulsed direct current mag¬netic fields (MFs) would cause a lipid peroxidation in the rat lung cells. Also, the experiment was carried out to investigate whether treatment with melatonin could block the effect of magnetic fields on lung tissue. Material and methods:Thirty rats were included in the study. The animals were divided into three groups (MF and MF+melatonin, controls) equally. The animals in the MF+melatonin group were injected with melatonin (4mg/kg, intraperitoneal) and exposed to a 27.17 MHz MFs, dummy-load was 50 Ohms for matched antennas impedance. Duration of exposure was 2.5 hours/day, 6days/week for two weeks. MF group were exposed to same magnetic fields at the same times. Control rats were injected with only physiologic saline solution (1mg/kg, intraperitoneal). After the experiment, the rats were sacrificed and thoracic cavity was explored; blood samples were obtained from the cardiac region and lungs were extracted and homogenised. MDA lev¬els were studied in all groups. Results: MDA levels were significantly higher in the MF group than the MF+melatonin group, for erythrocyte hemolysate 3.53 0.64 nmol/ml vs 1.72 0.64 nmol/ml (p=0.000), and for lung tissue 2.72 0.51 nmol/gr vs 1.44 0.28 nmol/gr (p=0.000), respectively. Conclusion: Melatonin provides significant protection for lung cells and erythrocytes from oxidative damage induced by MFs exposure.
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Abstract (Original Language): 
Giriş: Radyofrekans radyasyonuna maruz kalan küçük memelilerde melatonin sekresyonunda bir azalma gözlemlenmiştir. Radyofrekans elektromanyetik alanlar yayan 27.17 MHz band radyoların artan kullanımının sağlık üzerine olası etkileri hakkında endişeler olması sebebiyle bu alanların lipid peroksidasyonunun bir belirteci olarak yaygın kullanılan malondialdehid (MDA) düzeyinde değişiklik yapıp yapmadığını araştırdık. Amaç: Bu deneyde direkt manyetik alan (MA) akımına maruz kalan ratların akciğer hücrelerinde lipid per-oksidasyonun oluşup oluşmadığını ve manyetik alanların akciğer dokusu üzerindeki etkilerinin melatonin tedavisiyle engellenip engellenmediği belirlemek. Gereç ve yöntem: Çalışmaya otuz rat alındı ve hayvanlar üç gruba ( MA, MA+melatonin, kontrol) eşit olarak dağıtıldı. MA+Melatonin grubuna günlük 4mg/kg intraperitoneal melatonin enjekte edildi ve uyarlanmış anten impedansının kör-yükü 50 Ohm olan 27.17 MHz manyetik alana iki hafta boyunca, haftada altı gün, günde 2.5 saat maruz bırakıldı. MA grubu da aynı manyetik alana aynı süre maruz bırakıldı. Kontrol grubuna ise sadece 1 mg/kg fizyolojik salin solüsyonu intraperitoneal uygulandı. Deney sonunda tüm ratların toraks boşlukları açıldı. Kardiyak ponksiyon ile kan örnekleri alındı, akciğerler çıkarılarak homojenize edildi ve her üç grupta MDA düzeyleri çalışıldı. Bulgular: MDA düzeyleri eritrosit hemolizatında 3.53 0.64 nmol/ml, 1.72 0.64 nmol/ml (p=0.000) ve akciğer dokusunda 2.72 0.51 nmol/gr, 1.44 0.28 nmol/gr (p=0.000) olmak üzere MA grubunda MA+melatonin grubuna göre anlamlı olarak yüksek bulundu. Sonuç: Melatonin ratlarda MA maruziyetiyle oluşturulan oksidatif hasara karşı eritrositler ve akciğer hücreleri için anlamlı bir koruma sağlamaktadır.
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