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Tavşanlarda topikal penisilin uygulaması ile oluşturulan deneysel epilepsi sonucu beyin eser element değişiklikleri

The brain trace elements Changes in experimental epilepsy induced by topical penicillin application in rabbits

Journal Name:

  • Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi

Publication Year:

  • 2004

Key Words:

Keywords (Original Language):

Author NameUniversity of AuthorFaculty of Author
Abstract (2. Language): 
The aim of this study was to investigate the role of zinc (Zn) and copper (Cu) on physiopathology of epilepsy. For this purpose, experimental epilepsy model was formed by penicillin application in rabbits. Forty rabbits were used and divided into equal two groups as experimental epilepsy group and control group. Screw electrodes were applied in four different cortical regions in rabbits. Electrophysiological and clinical seizures were observed after 60000 IU penicillin dropped in posterior sensory motor area, and their electrocorti-gograms were recorded. The animals were decapitated two hours later and their brains were evaluated. Brains were homogenized in nitric acid solution and the levels of Zn and Cu were measured using atomic absorption device. We found that Zn levels in rabbits induced epilepsy were lower and Cu levels were higher than control groups . But these differences were not statistically significant. In conclusion, topical penicillin application had no significant effect Zn and Cu levels in brain of rabbits.
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Abstract (Original Language): 
Bu çalışmanın amacı çinko (Zn) ve bakırın (Cu ) epilepsi fizyopatolojisindeki rollerini araştırmaktır. Bunun için, tavşanlarda topikal penisilin uygulaması ile deneysel epilepsi oluşturuldu. Çalışma 20'si deneysel epilepsi grubu ve 20'si kontrol grubu olmak üzere 40 adet tavşan üzerinde gerçekleştirildi. Tavşanların dört farklı korteks alanına vida elektrotlar yerleştirildi. Posterior sensorimotor alana 60 bin ünite potasyum penisilin G damlatılması sonrasında elektrofizyolojik ve klinik nöbet gözlendi ve elektrokortigogramları kaydedildi. Nöbet gözlendikten iki saat sonra tavşanlar dekapite edilerek beyinleri çıkarıldı. Beyinler nitrik asitle homoj-enize edildikten sonra Zn ve Cu düzeyleri atomik absorbsiyon aleti ile ölçüldü. Epilepsi oluşturulan gruptaki Zn miktarlarında düşüklük, Cu miktarlarında ise yükseklik tespit edildi. Fakat bu değişiklikler istatistiksel olarak anlamlı değildi. Sonuç olarak tavşanlarda topikal penisilin uygulamasının beyin Zn ve Cu düzeylerini anlamlı düzeyde etkilemediği kanaatine varıldı.
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  • 4
10-13
  • Turkish

REFERENCES

References: 

1. Long VG. Electroencephalographic atlas for pharmacological research. Rabbit Brain Research. Volume II. Newyork, Elsevier 1962;1-13
2. Curtis DR, Game CJA, Mc Culloch MR, Maclachalan RM. Convulsive action of penicillin. Brain Research 1972;43:242-5
3. Harris GL, Harris AB, Wick C. Penicillin effects on cortex synaptic vesicle uptake of horseradish peroxidase. Brain Res 1979;161:361-6.
4. Kuzuya T, Hasegawa T, Shimizu K. Nabeshima T. Effect of antiepileptic drugs on serum zinc and copper concentra¬tions in epileptic patients. International Journal of Clinical Pharmacology. Therapy and Toxicology 1993;31(2):61-5
5. Dawidson DLW, Ward NI. Abnormal aluminium, cobalt, manganese, selenium and zinc concentrations in untreated epilepsy. Epilepsy Res 1988;2:323-30
6. Hiyashi A, Ikeda T, Matsukara M, Matudo I. Serum Zinc and vitamin E concentrations in handicapped children treat¬ed with anticonvulsants. Dev Pharmacol Ther 1982;5:109-
13
7.
İlha
n A, Özerol E, Güleç M, Işık B, İlhan N, İlhan N et al. The comparison of nail and serum trace elements in patients with epilepsy and healthy subjects. Progression Neuro-Psychopharmacology and Biologica Psychiatry 2004;28:
99-104
8. Barbean A, Donaldson J. Zinc, taurin and epilepsy. Arch
Neurol 1974; 30:52-8
9. Verrotti A, Basciani F, Trotta D, Pomilio MP, Morgese G, Chiarelli F. Serum copper, zinc, selenium, glutathione per-oxidase and superoxide dismutase levels in epileptic chil¬dren before and after 1 year of sodium valproate and carba-mazepine therapy. Epilepsy Res. 2002;48(1-2) :71-5.
10. Burhanoglu M, Tutuncuoglu S, Coker C, Tekgul H, Ozgur T. Hypozincaemia in febrile convulsion. Eur J Pediatr. 1996;155(6):498-501
11 .Kaji M, Ito M, Okuno T, Momoi T, Sasaki H, Yamanaka C et all. Serum copper and zinc levels in epileptic children with valproate treatment. Epilepsia 1992;33(3):555-7
12. Sozuer DT, Barutcu UB, Karakoc Y, Yalcin E, Onen S. The effects of antiepileptic drugs on serum zinc and copper levels in children. J Basic Clin Physiol Pharmacol.
1995;6(3-4):265-9
13. Chung SO, Johnson MS. Divalent transition metal ions in brains of epileptogenic and normal mice. Brain Research
1983;280: 323-34
14. Pei Y, Zhao D, Huang J, Cao L. Zinc induced seizures: A new experimental model of epilepsy. Epilepsia. 1983;24(2):169-76.
15. Le Quesne PM. Toxic substances and the nervous system: the role of clinical observation. J Neurol Neurosurg Psychiatry. 1981;44(1):1-8.
16. Monier M, Gangloff H. Atlas for Stereotaxic brain research. Rabbit Brain Research Volume I. Newyork
Elsevier Publishing 1961:8-26
17. Martin HJ. The collective electrical behaviour of cortical neurons: the electroencephalogram and the mechanism of epilepsy. In: Kandel ER, Schwartz JH, and Jessell TM. Eds. Principles of Neural Science. 3rd Ed., New York, Amsterdam, Elvesier Science Publishing 1991:77-791.
18. Sullivan HC, Osorio I. Aggravation of penicillin-induced epilepsy in rats with locus ceruleus lesions. Epilepsia 1991;32:591-6.
19. Walden J, Straub H, Speckmann EJ. Epileptogenesis: Contributions of calcium ions and antiepileptic calcium antagonists. Acta Neurologica Scandinavica (Suppl.) 1992;150:41-6.
20. Strerman MB, Shouse MN, Fairchild MD, Belsito O.
Kindled seizure induction alters and is altered by zinc absorption. Brain Research 1986,1:382-6
21. Marangoz C, Ağar E, Ayyıldız M, Taşcı N. Levels of zinc in the cerebral hemispheres following parenteral penicilin in rats. Journal of Islamic Academy of Science 1989;2:62-4

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